A study on the refolding of lysozyme using fed-batch and step-addition strategies

Journal of the Chinese Institute of Chemical Engineers

Volumn 35, Issue 4, 2004, Pages 389-398

  Wang, Steven S S ^a   Chang, Che Kuei ^a   Liu, Hwai Shen ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Fed batch; Lysozyme; Refolding; Step addition

Indexed keywords

AGGLOMERATION; BIOTECHNOLOGY; CHEMICAL ENGINEERING; OPTIMIZATION; PRODUCTIVITY;

FED-BATCH SCHEME; LYSOZYMES; PROTEIN AGGREGATES; RENATURATION;

ENZYME KINETICS;

BIOREACTOR;

EID: 4844220049     PISSN: 03681653     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Batas, D. and J. B. Chaudhuri, "Protein Refolding at High Concentration Using Size-Exclusion Chromatography," Biotechnol. Bioeng., 50(1), 16 (1996).
2. Buchner, J., I. Pastan, and U. Brinkmann, "A Method for Increasing the Yield of Properly Folded Recombinant Fusion Proteins: Single-Chain Immunotoxins from Renaturation of Bacterial Inclusion Bodies," Anal. Biochem., 205(2), 263 (1992).
3. Clark, E. D, "Protein Refolding for Industrial Processes," Current Opinion in Biotechnology, 12(2), 202 (2001).
4. Dobson, C. M., P. A. Evans, and S. E. Radford, "Understanding How Proteins Fold-The Lysozyme Story so Far," Trends Biochem. Sci., 19(1), 31 (1994).
5. Fahey, E. M., P. Binding, and J. B. Chaudhuri, "Size Exclusion Chromatography as a Tool for Refolding Recombinant Proteins," Abstracts of Papers of the American Chemical Society, 217, U871 (1999).
6. Goldberg, M. E., R. Rudolph, and R. Jaenicke, "A Kinetic-Study of the Competition between Renaturation and Aggregation during the Refolding of Denatured Reduced Egg-White Lysozyme," Biochemistry, 30(11), 2790 (1991).
7. Hamaker, K. H., J. Y. Liu, R. J. Seely, C. M. Ladisch, and M. R. Ladisch, "Chromatography for Rapid Buffer Exchange and Refolding of Secretory Leukocyte Protease Inhibitor," Biotechnol. Prog., 12(2), 184 (1996).
8. Kim, S., J. Baum, and S. Anderson, "Production of Correctly Folded Recombinant C-13, N-15-Enriched Guinea Pig Val90-Alpha-Lactalbumin," Protein Eng., 10(4), 455 (1997).
9. Liu, H. S. and C. K. Chang, "Chaperon Solvent Plug to Enhance Protein Refolding in Size Exclusion Chromatography," Enzyme Microb. Technol., 33, 424 (2003).
10. Matsubara, M., D. Nohara, E. Kurimoto, Y. Kuroda, and T. Sakai, "'Loose Folding' and 'Delayed Oxidation' Procedures Successfully Applied for Refolding of Fully Reduced Hen Egg White Lysozyme," Chem. Pharm. Bull. (Tokyo), 41(7), 1207 (1993).
11. Negro, A., M. Onisto, L. Grassato, C. Caenazzo, and S. Garbisa, "Recombinant Human TIMP-3 from Escherichia Coli - Synthesis, Refolding, Physico-Chemical and Functional Insights," Protein Eng., 10(5), 593 (1997).
12. West, S. M., J. B. Chaudhuri, and J. A. Howell, "Improved Protein Refolding Using Hollow-Fibre Membrane Dialysis," Biotechnol. Bioeng., 57(5), 590 (1998).