Purification and wash performance analysis of thermostable extracellular alkaline protease produced by soil bacterium Bacillus sp. GOS-2

Asian Journal of Microbiology, Biotechnology and Environmental Sciences

Volumn 10, Issue 1, 2008, Pages 29-35

  Selvakumar, R ^a   Sathish Kumar, R ^a   Swaminathan, K ^a  

^a BHARATHIAR UNIVERSITY   (India)

Author keywords

Alkaline protease; Detergent; Gel filtration chromatography

Indexed keywords

ACETONE; ALKALINE PROTEINASE; DETERGENT; OXIDIZING AGENT; STABILIZING AGENT; SURFACTANT;

ARTICLE; BACILLUS; BACTERIAL STRAIN; BACTERIUM ISOLATION; BLOOD STAIN; CONCENTRATION RESPONSE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; GEL FILTRATION CHROMATOGRAPHY; INDIA; INDUSTRY; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; SOIL MICROFLORA; TEMPERATURE; THERMOSTABILITY;

BACILLUS SP.; BACTERIA (MICROORGANISMS);

EID: 48349126076     PISSN: 09723005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Anwar, A. and Saleemuddin, M. 1997. Alkaline-pH acting digestive enzymes of the polyphagous insect pest Spi/osoma obliqua: stability and potential as detergent additives. Biotechnol. Appl. Biochem. 25(1): 43-46.
2. Banerjee, C., Sani, R.K., Azmi, W. and Sani R. 1991. Thermostable alkaline protease trom Bacillus brevis and its characterization as a laundry detergent additive. Process Biochem. 35: 213-219.
3. Belma Aslim., Necdet Saglam and Yavuz Beyatli. 2002. Determination of some properties of Bacillus isolated trom soil. Turk J. Biology. 26: 41-48.
4. Berla Thangam, E and Suseela Rajkumar, G. 2002. Purification and characterization of alkaline protease trom Alcaligenes faecalis. Biotechnol Appl Biochem. 35: 149-154.
5. Debette, J. 1991. Isolation and characterizaion of an extracellular protease produced by a soil strain of Xanthomonas maltophila. Curro Microbiol. 22: 85-90.
6. Dhandapani, Rand Vijayaraghavan, R. 1994. Production of thennophilic, extra cellular alkaline protease by B.Stearothermophilus AP-4. W. J.Microbiol. Biotechnol. 10: 33-35.
7. Fujiwara, N. and Yamamoto, K. 1987. Production of alkaline protease in low- cost medium by alkalophilic Bacillus sp and properties of the enzyme. J. Ferm. Technol. 65: 345-348.
8. Gupta, R., Beg, Q., Khan S. and Chauhan B., 2002. An overview on fennentation, downstream processing and properties of microbial alkaline protease. Appl. Microbiol. Biotechnol. 60: 381-395.
9. Hagihara, B., Matsubara, H., Nakai, M. and Okumuki, K. 1958. Crystalline bacterial protease I. Preperation of crystalline proteinase of Bacillus substilis. J. Biochem. 45: 185-194.
10. Joo H.S., Kumar, C.G., Park G.C., Paik, S.R. and Chang C.S. 2003. Oxidant and SDS- stable alkaline protease from Bacillus clausii I-52: production and some properties. J. Appl. Microbiol. 95: 267-272.
11. Kobayashi, T., Hakamada, Y., Adachi, S., Hitomi, J., Yoshimatsu, T., Koike, K., S. Kawai and Ito, S. 1995. Purification and properties of an alkaline protease from alkalophilic Bacillus sp KSM-16 Appl. Microbiol. Biotechnol. 43: 473-481.
12. Kunamneni Adinarayana., Polouri Ellaiah and Davuluri Sivaprasad. 2003. Purification and partial characterization of thennostable serine alkaline protease nom a newly isolated Bacillus substi/is PE-ll. AAPS. Pharma. Sci. Technol. 4(4): 56, 1-9.
13. Kwon, Y.T, Kim, 1.0, Moon, S.Y, Lee, H.H and Rho H.M. 1994. Extra cellular alkaline protease nom alkalophilic Vibrio metschnikovi strain RH530. Biotechnol. Letters. 16: 413-418.
14. Laemmli, U.K. 1971. Cleavage of structural proteins during the assembly of the head of bacterial phage T4. Nature. 227: 680-685.
15. Lowry, O.H., Rosebrough, N., Farr, A.L. and Ronadall R.L. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chern. 193: 265-273.
16. Mohsen Fathi Najafi., Dileep Deobagkar and Deepti Deobagkar. 2005. Potential application of protease isolated nom Pseudomonas aeruginosa PD 100. J. Biotechnol. 8 (2): 197-203.
17. Oberoi, R., Beg, Q.K., Puri, S., Saxena, R.K. and Gupta, R. 2001. Characterization and wash perfonnance analysis of an SDS- stable alkaline protease nom a Bacillus sp. W. J. Microbiol. Biotechnol. 17: 493-497.
18. Outtrup, H. 1990. Boyee Col. Microbial proteases and Biotechnology. Elseiver Science, New York, 227-254.
19. Rathindra Mohan Banik and Monika Prakash. 2004. Laundry detergent compatibility of the alkaline protease nom Bacillus cereus, Microbiol. Res. 159: 135-140.
20. Saeki, K., Hitomi, J., Okuda, M., Hatada, Y., Kageyama, Y., Takaiwa, M., Kubota, H., Hagihara, H., Kobayashi, T., Kawai, S. and Ito, S. 2002. A novel species of alkalophilic Bacillus that produces an oxidatively stable serine protease. Extremophiles. 6: 65-72.
21. Walsh, K.A. and Wilocox, P.E. 1970. Methods in Enzymology. pp. 31-226. In Perlmann, G.E., Lonand, L. (eds.). Vol. 19, Academic press, New York.