Photoaffinity labeling and mutational analysis of 24-C-sterol methyltransferase defines the AdoMet binding site

Lipids

Volumn 43, Issue 8, 2008, Pages 681-693

  Jayasimha, Pruthvi ^a   Nes, W David ^a  

^a TEXAS TECH UNIVERSITY   (United States)

Author keywords

AdoMet dependent methyl transferase; Affinity labeling; Mutagenesis; Rossmann fold; Saccharomyces cerevisiae; Sterol methyltransferase; Zymosterol

Indexed keywords

ALANINE; AMINO ACID; ASPARTIC ACID; CYSTEINE; GLYCINE; LEUCINE; METHYLTRANSFERASE; PROLINE; RADIOACTIVE MATERIAL; SINEFUNGIN; STEROL; TRYPSIN; TYROSINE; VALINE;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; BIOINFORMATICS; CATALYSIS; ENZYME ACTIVE SITE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IRRADIATION; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOTOAFFINITY LABELING; POLYACRYLAMIDE GEL ELECTROPHORESIS; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

BINDING SITES; DNA MUTATIONAL ANALYSIS; METHYLTRANSFERASES; MOLECULAR PROBES; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; PEPTIDE HYDROLASES; PHOTOAFFINITY LABELS; S-ADENOSYLMETHIONINE; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT;

SACCHAROMYCES CEREVISIAE;

EID: 48349102660     PISSN: 00244201     EISSN: 15589307     Source Type: Journal    
DOI: 10.1007/s11745-008-3198-x     Document Type: Article

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