Molecular characterization of phytocystatins isolated from Chinese cabbage flower buds

Genes and Genomics

Volumn 30, Issue 3, 2008, Pages 235-243

  Hong, Joon Ki ^b   Hwang, Jung Eun ^a   Lim, Chan Ju ^a   Lee, Kyun Oh ^a   Chung, Woo Sik ^a   Park, Beom Seok ^b   Lim, Chae Oh ^a  

^a Gyeongsang National University   (South Korea)

^b National Institute of Animal Science   (South Korea)

Author keywords

Cysteine peptidase; Inhibitory activity; Recombinant protein; Stability

Indexed keywords

BRASSICA RAPA SUBSP. PEKINENSIS;

EID: 48349097490     PISSN: 19769571     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (39)

1. 2-terminal 21 amino acid residues are not essential for the papain-inhibitory activity of oryzacystatin, a member of the cystatin superfamily. J. Biol. Chem. 263: 7655-7659.
2. Abe K, Emori Y, Kondo H, Suzuki K and Ami S (1987a) Molecular cloning of a cysteine proteinase inhibitor of rice (oryzacystatin). Homology with animal cystatins and transient expression in theripening process of rice seeds. J. Biol. Chem. 262: 16793-16797.
3. Abe K, Kondo H and Arai S (1987b) Purification and characterization of a rice cysteine proteinase inhibitor. Agric. Biol. Chem. 51: 2763-2768.
4. Abe M, Abe K, Iwabuchi K, Domoto C and Arai S (1994) Corn cystatin I expressed in Escherichia coli: investigation of its inhibitory profile and occurrence in corn kernels. J. Biochem. 116: 488-492.
5. Belenghi B, Acconcia F, Trovato M, Perazzolli M, Bocedi A, Polticelli F, Ascenzi P and Delledonne M (2003) AtCYS1, a cystatin from Arabidopsis thaliana, suppresses hypersensitive cell death. Eur. J. Biochem. 270: 2593-2604.
6. Bode W, Engh R, Musil D, Thiele U, Huber R, Karshikov A, Brzin J, Kos J and Turk V (1988) The 2.0 Å X-ray crystal structure of chicken egg-white cystatin and its possible mode of interaction with cysteine proteinases. EMBO J. 7: 2593-2599.
7. Bonade-Bottino M, Lerin J, Zaccomer B and Jouanin L (1999) Physiological adaptation explains the insensitivity of Baris coerulescens to transgenic oilseed rape expressing oryzacystatin I. Insect Biochem. Mol. Biol. 29: 131-138.
8. Brzin J, Rogelj B, Popovic T, Strukelj B and Ritonja A (2000) Clitocypin, a new type of cysteine proteinase inhibitor from fruit bodies of mushroom Clitocybe nebularis. J. Biol. Chem. 275: 20104-20109.
9. Calderini O, Bovone T, Scotti C, Pupilli F, Piano E and Arcioni S (2007) Delay of leaf senescence in Medicago sativa transformed with the ipt gene controlled by the senescence-specific promoter SAG12. Plant Cell Rep. 26: 611-615.
10. Corre-Menguy F, Cejudo FJ, Mazubert C, Bidal J, Lelandais- Brière C, Torres G, Rode A and Hartmann C (2002) Characterization of the expression of a wheat cystatin gene during caryopsis development. Plant Mol. Biol. 50: 687-698.
11. Dixon M, Webb EC, Throne CJR and Tipton KF (1979) Enzyme inhibition and activation. In Enzymes, Academic Press, New York, pp. 332-381.
12. Gaddour K, Vicente-Carbajosa J, Lara P, Isabel-Lamoneda I, Diaz I and Carbonero P (2001) A constitutive cystatin-encoding gene from barley (lcy) responds differentially to abiotic stimuli. Plant Mol. Biol. 45: 599-608.
13. Halfon S, Ford J, Foster J, Dowling L, Lucian L, Sterling M, Xu Y, Weise M, Ikeda M, Liggett D, Helms A, Caux C, Lebecque S, Hannum C, Menon S, McClanahan T, Gorman D and Zurawaski G (1998) Leukocystatin, A New Class II Cystatin Expressed Selectively by Hematopoietic Cells. J. Biol. Chem. 273: 16400-16408.
14. Hong JK, Hwang JE, Lim CJ, Yang KA, Jin Z-L, Kim CY, Koo JC, Chung WS, Lee KO, Lee SY, Cho MJ and Lim CO (2007) Over-expression of Chinese cabbage phytocystatin 1 retards seed germination in Arabidopsis. Plant Sci. 172: 556-563.
15. Hong JK, Lee EY, Jin ZL, Kim HI, Hong JC, Choi YJ, Yun D-J, Lee SY, Bank JD, Cho MJ and Lim CO (2002) Molecular characterization of cDNA encoding the ADP-ribosylation factor like-protein (ARL), BcARF3, from Chinese cabbage flower bud. Korean J. Genetics 24: 367-376.
16. Jin ZL, Ryu SH, Hong JK, Song CE, Lim CJ, Choi YJ, Lee KO, Chung WS, Lee SY and Lim CO (2007) Over-expression of Chinese cabbage calreticulin 1b, BrCRT1b, induces initiation of organogenesis in Arabidopsis. Korean J. Genetics 29: 409-418.
17. Kloetzel JA, Baroin-Tourancheau A, Miceli C and Aubusson A (2003) Cytoskeletal proteins with N-terminal signal peptides: plateins in the ciliate Euplotes define a new family of articulins. J. Cell Sci. 116: 1291-1303.
18. Kondo H, Abe K, Emori Y and Arai S (1991) Gene organization of oryzacystatin-II, a new cystatin superfamily member of plant origin, is closely related to that of oryzacystatin-I but different from those of animal cystatins. FEBS Lett. 278: 87-90.
19. Kondo H, Abe K, Nisbimura I, Watanabe H, Emori Y and Arai S (1990) Two distinct cystatin species in rice seeds with different specificities against cysteine proteinases: molecular cloning, expression, and biochemical studies on oryzacystatin-II. J. Biol. Chem. 265: 15832-15837.
20. Kouzuma Y, Inanaga H, Doi-Kawano K, Yamasaki N and Kimura M (2000) Molecular cloning and functional expression of cDNA encoding the cysteine proteinase inhibitor with three cystatin domains from sunflower seeds. J. Biochem. 128: 161-166.
21. Kouzuma Y, Kawano K, Kimura M, Yamasaki N, Kadowaki T and Yamanoto K (1996) Purification, characterization, and sequencing of two cysteine proteinase inhibitors, Sea and Scb, from sunflower (Helianthus annuus) seeds. Biochem. 119: 1106-1113.
22. Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
23. Lecardonnel A, Chauvin L, Jouanin L, Beaujean A, Prevost G and Sangwan-Norreel B (1999) Effects of rice cystatin I expression in transgenic potato on Colorado potato beetle larvae. Plant Sci. 140: 71-79.
24. Lim CO, Kim HY, Kim MG, Lee SI, Chung WS, Park SH, Hwang I and Cho MJ (1996a) Expressed sequence tags of Chinese cabbage flower bud cDNA. Plant Physiol. 111: 577-588.
25. Lim CO, Lee SI, Chung WS, Park SH, Hwang I and Cho MJ (1996b) Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Plant Mol. Biol. 30: 373-379.
26. Margis R, Reis EM and Villeret V (1998) Structural and phylogenetic relationships among plant and animal cystatins. Arch. Biochem. Biophys. 359: 24-30.
27. Rassam M and Laing WA (2004) Purification and characterization of phytocystatins from kiwifruit cortex and seeds. Phytochem. 65: 19-30.
28. Rawlings ND, Tolle DP and Barrett AJ (2004) Evolutionary families of peptidase inhibitors. Biochem. J. 378: 705-716.
29. Rogelj B, Popovič T, Ritonja A, Strukelj B and Brzin J (1998) Chelidocystatin, a novel phytocystatin form Chelidonium majus. Phytochem. 49: 1645-1649.
30. Siqueira-Júior CL, Fernandes KVS, Machado OLT, Cunha MD, Gomes VM, Moura D and Jacinto T (2002) 87 kDa tomato cystatin exhibits properties of a defense protein and forms protein crystals in prosystemin overexpressing transgenic plants. Plant Physiol. Biochem. 40: 247-254.
31. Soares-Costa A, Beltramini LM, Thiemann OH and Henrique-Silva F (2002) A sugarcane cystatin: recombinant expression, purification, and antifungal activity. Biochem. Biophys. Res. Commun. 296: 1194-1199.
32. Song I, Taylor M, Baker K and Bateman RC (1995) Inhibition of cysteine proteinases by Carica papaya cystatin produced in Escherichia coli. Gene 162: 221-224.
33. Shyu DJM Chyan CL, Tzen JTC and Chou WM (2004) Molecular cloning, Expression, and Functional Characterization of a Cystatin from Pineapple Stem. Biosci. Biotechnol. Biochem. 68: 1681-1689.
34. Stubbs MT, Laber B, Bode W, Huber R, Jerala R, Lenarcic B and Turk V (1990) The refined 2.4 Å X-ray crystal structure of recombinant human stefin B in complex with the cysteine proteinase papain: a novel type of proteinase inhibitor interaction. EMBO J. 9: 1939-1947.
35. Turk B, Krizaj I, Kralj B, Dolenc I, Popovic T, Bieth JG and Turk V (1993) Bovine stefin C, a new member of the stefin family. J. Biol. Chem. 268: 7323-7329.
36. Turk V and Bode W (1991) The cystatins: Protein inhibitors of cysteine proteinases. FEBS Lett. 285: 231-219.
37. Vain P, Worland B, Clarke MC, Richard G, Beavis M, Liu H, Kohli A, Leech M, Snape J, Christou P and Atkinson H (1998) Expression of an engineered cysteine proteinase inhibitor (Oryzacystatin-IΔ86) for nematode resistance in transgenic rice plants. Theor. Appl. Genet. 96: 266-271.
38. Wu J and Haard NF (2000) Purification and characterization of a cystatin from the leaves of methyl jasmonate treated tomato plants. Comparative Biochem. Physiol. Part C 127: 209-220.
39. Zhao Y, Botella MA, Subramanian L, Niu X, Nielsen SS, Bressan RA and Hasegawa PM (1996) Two wound-inducible soybean cysteine proteinase inhibitors have greater insect digestive proteinase inhibitory activities than a constitutive homolog. Plant Physiol. 111: 1299-1306.