Efficient method for slow cryopreservation of human embryonic stem cells in xeno-free conditions

Reproductive BioMedicine Online

Volumn 17, Issue 1, 2008, Pages 127-135

  Valbuena, Diana ^a   Sánchez Luengo, Silvia ^a   Galán, Amparo ^a   Sánchez, Eva ^a   Gómez, Eva ^a   Poo, M Eugenia ^a   Ruiz, Verónica ^a   Genbacev, Olga ^b   Krtolica, Ana ^c   Pellicer, Antonio ^b   Moreno, Rubén ^a   Simón, Carlos ^a,b  

^a CENTRO DE INVESTIGACIÓN PRÍNCIPE FELIPE   (Spain)

^b UNIVERSITY OF VALENCIA   (Spain)

^c StemLifeLine   (United States)

Author keywords

Cryopreservation; Dimethylsulphoxide; Human embryonic stem cells

Indexed keywords

DIMETHYL SULFOXIDE; GENE PRODUCT; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; OCTAMER TRANSCRIPTION FACTOR 4; PROTEIN AMI; PROTEIN CR1; PROTEIN NFH; PROTEIN REN; PROTEIN TDGF1; TELOMERASE; THY 1 ANTIGEN; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL LINE; CELL STRUCTURE; CELL SURVIVAL; CELL TYPE; CONCENTRATION RESPONSE; CONTROLLED STUDY; COOLING; CRYOPRESERVATION; EMBRYO; EMBRYONIC STEM CELL; ENZYME ACTIVITY; FREEZE THAWING; GROWTH RATE; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; KARYOTYPE; MOUSE; NONHUMAN; SEVERE COMBINED IMMUNODEFICIENCY; SURVIVAL RATE; TERATOMA; VITRIFICATION;

EID: 48249108747     PISSN: 14726483     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1472-6483(10)60302-1     Document Type: Article

References (16)

1. Cooper DKC 2000 Xeno: The Promise of Transplanting Animal Organs into Humans. Oxford University Press, New York.
2. Gosden R 1992 Transplantation of ovaries and testes. In: Edwards RG (ed.) Fetal Tissue Transplants in Medicine. Cambridge University Press, Cambridge, pp. 253-279.
3. Ha SY, Jee BC, Suh CS et al. 2005 Cryopreservation of human embryonic stem cells without the use of a programmable freezer. Human Reproduction 20, 1779-1785.
4. Ludwig TE, Levesnstein ME, Jones JM et al. 2006 Derivation of human embryonic stem cells in defined conditions. Nature Biotechnology 24, 185-187.
5. Morris GJ, Acton E, Faszer K et al. 2006 Cryopreservation of murine embryos, human spermatozoa and embryonic stem cells using a liquid nitrogen-free, controlled rate freezer. Reproductive BioMedicine Online 13, 421-426.
6. Pegg DE 2005 The role of vitrification techniques of cryopreservation in reproductive medicine. Human Fertility 8, 231-239.
7. Rall WF, Fahy GM 1985 Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification. Nature 313, 573-575.
8. Reubinoff BE, Pera MF, Vajta G et al. 2001 Effective cryopreservation of human embryonic stem cells by open straw vitrification method. Human Reproduction 16, 2187-2194.
9. Richards M, Fong CY, Tan S et al. 2004 An efficient and safe xenofree cryopreservation method for the storage of human embryonic stem cells. Stem Cells 22, 779-789.
10. Robertson EJ 1987 Embryo derived stem cell lines. In: Robertson EJ (ed.) Teratocarcinomas and Embryonic Stem Cells: A Practical Approach. IRL Press, Oxford, pp. 71-112.
11. Rodríguez C, Simón C 2006 Human embryonic stem cell derivation: from the IVF perspective to therapeutic applications. Regenerative Medicine 1, 103-109.
12. Rodríguez C, Galán A, Valbuena D, Simón C 2006 Derivation of clinical-grade human embryonic stem cells. Reproductive BioMedicine Online 12, 112-118.
13. Thompson JA, Itskovitz-Eldor J, Shapiro SS et al. 1998 Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147.
14. Trounson A, Mohr L 1983 Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature 305, 707-709.
15. Valbuena D, Galán A, Sanchez E et al. 2006 Derivation and characterization of three new Spanish human embryonic stem cell lines (VAL -3 -4 -5) on human feeder and in serum-free conditions. Reproductive BioMedicine Online 13, 875-876.
16. Whittingham D, Leibo S, Mazur P 1972 survival of mouse embryos frozen to -196°C and -269°C. Science 178, 411-414.