Visualizing nuclei in skin cryosections: Viable options to 4′6-diamidino-2-phenylindol for confocal laser microscopy

Skin Research and Technology

Volumn 14, Issue 3, 2008, Pages 324-326

  Glaser K ^a   Wilke, K ^a   Wepf, R ^a   Biel, Stefan ^a  

^a BEIERSDORF AG   (Germany)

Author keywords

Confocal laser scanning microscopy; Fixation; Fluorescence microscopy; Nuclear staining; Skin

Indexed keywords

CONFOCAL MICROSCOPY; IMAGING TECHNIQUES; LASER EXCITATION; LASERS; MICROSCOPIC EXAMINATION; PAINTING; PULSED LASER DEPOSITION; STANDARDS;

CELLULAR DISTRIBUTIONS; CONFOCAL LASER MICROSCOPY (CLM); CONFOCAL LASER SCANNING MICRO SCOPY (CLSM); EPIFLUORESCENCE MICROSCOPY (EFM); HUMAN SKIN; IMMUNOHISTOCHEMICAL STUDIES; SKIN SAMPLES; TRANSITION ZONES; ULTRAVIOLET (UV);

SKIN;

4',6 DIAMIDINO 2 PHENYLINDOLE; CELL NUCLEUS DNA; CYANINE DYE; DYE; IODIDE; PHENANTHRIDINE DERIVATIVE; PROPIDIUM IODIDE; RNA;

ARTICLE; BLEACHING; CELL NUCLEUS; CONCENTRATION (PARAMETERS); CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; FROZEN SECTION; HUMAN; HUMAN TISSUE; INCUBATION TIME; LASER; SKIN CELL; STAINING; ULTRAVIOLET RADIATION;

CELL NUCLEUS; CONTRAST MEDIA; CRYOULTRAMICROTOMY; DERMOSCOPY; HUMANS; IMAGE ENHANCEMENT; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; ORGANIC CHEMICALS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SKIN;

EID: 48249098992     PISSN: 0909752X     EISSN: 16000846     Source Type: Journal    
DOI: 10.1111/j.1600-0846.2008.00298.x     Document Type: Article

References (2)

1. Takato K, Hirano H. Use of fluorescein-phalloidin and DAPI as a counterstain for immunofluoerscence microscopic studies of semithin frozen sections. Acta Histochem Cyctochem 1990; 23: 679-683.
2. Suzuki T, Fujikura K, Higashiyama T, Takata K. DNA staining for fluorescence and laser microscopy. J Histochem Cytochem 1997; 45: 49-53.