Elimination of competing hydrolysis and coupling side reactions of a cyclodextrin glucanotransferase by directed evolution

Biochemical Journal

Volumn 413, Issue 3, 2008, Pages 517-525

  Kelly, Ronan M ^a,b   Leemhuis, Hans ^a,b   Rozeboom, Henriëtte J ^a   Van Oosterwijk, Niels ^a   Dijkstra, Bauke W ^a   Dijkhuizen, Lubbert ^a,b  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Amylase; Cyclodextrin glucanotransferase (CGTase); Protein evolution; Protein stability; Saccharide; Side reaction

Indexed keywords

AMINES; CRYSTAL STRUCTURE; CYCLIZATION; CYCLODEXTRINS; ENZYME ACTIVITY; ENZYMES; FOOD ADDITIVES; HYDROLYSIS; INTEGRAL EQUATIONS; PLANTS (BOTANY); SILANES; SUGAR (SUCROSE); SUGARS;

ACTIVE SITES; ANALYSIS OF VARIOUS; BREAKDOWN (BD); CATALYTIC FUNCTIONS; CATALYTIC SITES; CONFORMATIONAL CHANGES; CYCLIZATION ACTIVITY; CYCLODEXTRIN (CYDS); CYCLODEXTRIN GLUCANOTRANSFERASE (CGTASE); DIRECTED EVOLUTION; ENZYME FUNCTIONING; ERROR PRONE PCR; GLYCOSIDE HYDROLASES (GH); HYDROLYSIS REACTIONS; HYDROLYTIC ACTIVITIES; PEPTIDE BACKBONES; SIDE CHAINS; SIDE REACTIONS; TRANS GLYCOSYLATION ACTIVITY;

RATE CONSTANTS;

ALPHA CYCLODEXTRIN; BETA CYCLODEXTRIN; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; GAMMA CYCLODEXTRIN; GLYCOSIDASE; MUTANT PROTEIN;

ARTICLE; CHEMICAL REACTION; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; CYCLIZATION; ELIMINATION REACTION; ENZYME ACTIVE SITE; ENZYME CONFORMATION; GLYCOSYLATION; HYDROLYSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN FAMILY; REDUCTION; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

CALORIMETRY, DIFFERENTIAL SCANNING; CATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; EVOLUTION, MOLECULAR; GLUCOSYLTRANSFERASES; HYDROLYSIS; MODELS, MOLECULAR; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTEIN STRUCTURE, SECONDARY;

THERMOANAEROBACTERIUM THERMOSULFURIGENES;

EID: 48249092786     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20080353     Document Type: Article

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