Species-specific immunocytochemical localization of Mycobacterium tuberculosis complex in fine needle aspirates of tuberculous lymphadenitis using antibody to 38 kDa immunodominant protein antigen

Acta Cytologica

Volumn 52, Issue 4, 2008, Pages 424-433

  Goel, Madhu Mati ^a   Budhwar, Puja ^a  

^a KING GEORGE'S MEDICAL UNIVERSITY   (India)

Author keywords

Antimycobacterial agents; Aspiration cytology, fine needle; Immunocytochemistry; Lymphadenitis; Monoclonal antibody, Mycobacterium tuberculosis; Tuberculosis

Indexed keywords

ANTIGEN; STREPTAVIDIN;

ACID FAST BACTERIUM; ADULT; ARTICLE; ASPIRATION BIOPSY; CONTROL GROUP; CONTROLLED STUDY; FEMALE; HUMAN; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; MAJOR CLINICAL STUDY; MALE; MYCOBACTERIUM TUBERCULOSIS; PRIORITY JOURNAL; SCHOOL CHILD; SENSITIVITY AND SPECIFICITY; SPUTUM SMEAR; STAINING; TUBERCULOUS LYMPHADENITIS;

ATYPICAL MYCOBACTERIUM; BACILLI (CLASS); BACTERIA (MICROORGANISMS); MYCOBACTERIUM TUBERCULOSIS; MYCOBACTERIUM TUBERCULOSIS COMPLEX;

EID: 48149112800     PISSN: 00015547     EISSN: 19382650     Source Type: Journal    
DOI: 10.1159/000325547     Document Type: Article

References (25)

1. Goel MM, Budhwar P, Goel M, Tiwari V, Jain A: Nucleic acid amplification of Mycobacterium tuberculosis complex DNA from archival fine needle aspiration smear scrapings vs. fresh fine needle aspirates of tuberculous lymphadenitis. Acta Cytol 2006;50:393-397
2. Hazbon MH: Recent advances in molecular methods for early diagnosis of tuberculosis and drug resistant tuberculosis. Biomedica (Suppl) 2004;24:149-162
3. Goel MM, Ranjan V, Tapan DN, Srivastava AN, Mehrotra A, Khushwaha MRS, Jain A: Polymerase chain reaction vs. conventional diagnosis in fine needle aspirates of tuberculous lymph nodes. Acta Cytol 2001;45:333-340
4. Goel MM, Budhwar P: Immunohistochemical localization of Mycobacterium tuberculosis complex antigen with antibody to 38 kDa antigen versus Ziehl Neelsen staining in tissue granulomas of extrapulmonary tuberculosis. Indian J Tuberc 2007;54:24-29
5. Mukherjee A, Kalra N, Beena KR: Immunohistochemical detection of mycobacterial antigen in tuberculous lymphadenitis. Indian J Tuberc 2002;49:213-216
6. Higuchi S, Moritaka S, Dannenberg AM Jr, Affronti LF, Azuma I, Daniel TM, Petriali JP: Persistence of protein, carbohydrate and wax components of tubercle bacilli in dermal BCG lesions. Am Rev Respir Dis 1981;123:397-401
7. Humphrey DM, Weiner MH: Mycobacterial antigen detection by immunohistochemistry in pulmonary tuberculosis. Hum Pathol 1987;18:701-708
8. Barbolini G, Bisetti A, Colizzi V, Dalmiani G, Migaldi M, Vismara D: Immunohistologic analysis of mycobacterial antigens by monoclonal antibodies in tuberculosis and mycobacteriosis. Hum Pathol 1989;20:1078-1083
9. Luo D: Immunohistochemical demonstration of mycobacterial antigen. Zhonghua Jie He He Hu Xi Za Zhi 1990;13:360-362, 381-382
10. Wiley EL, Mulhollan TJ, Beck, Tyndall JA, Freeman RG: Polyclonal antibodies raised against bacillus Calmette-Guérin, Mycobacterium duvalii, and Mycobacterium paratuberculosis used to detect mycobacteria in tissue with the use of immunohistochemical techniques. Am J Clin Pathol 1990;94:307-312
11. Orelle JM, Brett SJ, Ivanyi J, Coghill G, Grant A, Beck JS: Measurement of the tissue distribution of immunoperoxidase staining with polyclonal anti-BCG serum in lung granulomata of mice infected with mycobacterium tuberculosis. J Pathol 1991;164:41-45
12. Radhakrishnan W, Mathai A, Radhakrishnan NS, Rout D, Sehgal S: Immunohistochemical demonstration of mycobacterial antigens in intracranial tuberculoma. Indian J Exp Biol 1991;29:641-644
13. Kutzner H, Argenyi ZB, Requena L, Rutten A, Hugel H: A new application of BCG antibody for rapid screening of various tissue microorganisms. J Am Acad Dermatol 1998;38:56-60
14. Oliveira BFT, Takay FC, Shida TM, Santo RM, Souza AC, Matayoshi S: Orbital tuberculosis diagnosed by immunohistochemistry: Case reports. Rev Inst Med Trop S Paulo 2004;46:291-294
15. Ulrichs T, Lefmann M, Reich M, Lars M, Roth A, Brinkmann V, Kosmiadi GA, Seiler P, Aichele P, Hahn H, Krenn V, Gobel UB, Kaufmann SHE: Modified immunohistological staining allows detection of Ziehl-Neelsen negative Mycobacterium tuberculosis organisms and their precise localization in human tissues. J Pathol 2005;205:633-640
16. Padmavathy L, Rao LL, Ramanadhan, Shakila: Mycobacterial antigen in tissues in diagnosis of cutaneous tuberculosis. Indian J Tuberc 2005;52:31-35
17. Mustafa T, Wiker HG, Mfinanga SGM, Morkve O, Sviland L: Immunohistochemistry using Mycobacterium tuberculosis complex specific antibody for improved diagnosis of tuberculous lymphadenitis. Mod Pathol 2006;19:1606-1614
18. Andersen AB, Hansen EB: Structure and mapping of antigenic domains of protein antigen b, a 38,000-molecular-weight protein of Mycobacterium tuberculosis. Infect Immun 1989;57:2481-2488
19. Ahmad A, Afghan S, Raykundalia C, Catty D: Diagnosis of tuberculosis by using ELISA to detect 38 kDa mycobacterial antigen in the patients. J Isl Aca Sci 1995;8:155-160
20. Ohtomo K, Sakamoto S, Umino T, Aoki T, Fukushima K, Kawabata Y, Iwai K, Kawamata K, Nakajima Y: Immunohistochemical studies on mycobacteria in tuberculous necrotizing granulomas. Kekkaku 1994;69:295-300
21. Furak J, Trojan I, Szoke T, Tiszlavicz L, Boda K, Balogh A, Roth E: Histological and immunohistochemical structure of pulmonary tuberculotic granulomas in untreated cases and cases treated with antitubercular drugs. Orv Hetil 2003;144:1347-1352
22. Kamboj SS, Goel MM, Tandon P, Natu SM, Nath P: Correlation study of histopathology and bacteriology in patients of tubercular lymphadenitis. Ind J Chest Dis Allied Sci 1994;36:187-191
23. Das DK, Pant JN, Chachra KL, Murthi NS, Satynarayan L, Thankamma TC, Kakkar PK: Tuberculous lymphadenitis: Correlation of cellular components and necrosis in lymph node aspirate with AFB positivity and bacillary count. Indian J Pathol Microbiol 1990;33:1-10
24. Pandit AA, Khilnani PH, Prayag AS: Tuberculous lymphadenitis: Extended cytomorphologic features. Diagn Cytopathol 1995;12:23-27
25. Thangaraj HS, Bull TJ, De Smet KA, Hill MK, Rouse DA, Moreno C, Ivanyi J: Duplication of genes encoding the immunodominant 38 kDa antigen in Mycobacterium intracellulare. FEMS Microbiol Lett 1996;144:235-240