Direct comparison of post-28-cycle PCR purification and modified capillary electrophoresis methods with the 34-cycle "low copy number" (LCN) method for analysis of trace forensic DNA samples

Forensic Science International: Genetics

Volumn 2, Issue 4, 2008, Pages 318-328

  Forster, Luke ^a   Thomson, Jim ^a   Kutranov, Stefan ^a  

^a LGC   (United Kingdom)

Author keywords

Capillary electrophoresis; LCN; Low copy number; Post PCR purification; Short tandem repeats; STRs; Trace DNA

Indexed keywords

ANALYTIC METHOD; ANALYTICAL PARAMETERS; ARTICLE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DNA DETERMINATION; DNA FINGERPRINTING; DNA ISOLATION; DNA MODIFICATION; DNA TEMPLATE; FORENSIC GENETICS; FORENSIC IDENTIFICATION; GENE AMPLIFICATION; GENE FREQUENCY; GENE NUMBER; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; LOW COPY NUMBER; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SAMPLING;

DNA; ELECTROPHORESIS, CAPILLARY; EPIDERMIS; FORENSIC GENETICS; HUMANS; MALE; MICROSATELLITE REPEATS; POLYMERASE CHAIN REACTION; SEMEN; SENSITIVITY AND SPECIFICITY; TEMPLATES, GENETIC; TOUCH;

EID: 48049124342     PISSN: 18724973     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fsigen.2008.04.005     Document Type: Article

References (18)

1. Gill P., Whitaker J.P., Flaxman C., Brown N., and Buckleton J. An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA. Forensic Sci. Int. 112 (2000) 17-40
2. ® SGM Plus™ multiplex system for both standard and low copy number (LCN) STR DNA analysis. Forensic Sci. Int. 123 (2001) 215-223
3. Gill P. Application of low copy number DNA profiling. Croat. Med. J. 42 (2001) 229-232
4. Findlay I., Frazier R., Taylor A., and Urquhart A. Single cell DNA fingerprinting for forensic applications. Nature 389 (1997) 555-556
5. BBC News Online, DNA test halted after Omagh case, BBC News Online, http://news.bbc.co.uk/1/hi/uk/7156051.stm, 23 December, 2007.
6. J. Booth and agencies, Wearside Jack pleads guilty to Ripper hoax, The Times, March 20, 2006.
7. Chris Summers, How science caught up with killer, BBC News online, http://news.bbc.co.uk/1/hi/uk/1459056.stm, 24 August, 2001.
8. Crown Prosecution Service, Review of the use of Low Copy Number DNA analysis in current cases: CPS statement, http://www.cps.gov.uk/news/pressreleases/101_08.html, 14 January, 2008.
9. Budowle B., Hobson D.L., Smerick J.B., and Smith J.A.L. Low Copy Number-consideration and caution. Proceedings of the Twelfth International Symposium on Human Identification (2001), Promega Corporation, Madison, Wisconsin. http://www.promega.com/geneticidproc/ussymp12proc/contents/budowle.pdf Available at:
10. Prinz M., Schiffner L., Sebestyen J.A., Bajda E., Tamariz J., Shaler R.C., Baum H., and Caragine T. Maximization of STR DNA typing success for touched objects. International Congress Series, vol. 1228 (2006) 651-653
11. Smith P.J., Ballantyne M.S., and Ballantyne J. Simplified Low-Copy-Number DNA analysis by post-PCR purification. J. Forensic Sci. 52 (2007) 820-829
12. Kloosterman A.D., and Kersbergan P. Efficacy of limits of genotyping low copy number DNA samples by multiplex PCR of STR loci. International Congress Series, vol. 1239 (2003) 795-798
13. ® SGM Plus™ system for use in forensic casework. Forensic Sci. Int. 112 (2000) 151-161
14. Taberlet P., Griffin S., Goossens B., Questiau S., Manceau V., Escaravage N., Waits L.P., and Bouvet J. Reliable genotyping of samples with very low DNA quantities using PCR. Nucleic Acids Res. 24 (1996) 1389-3194
15. Clayton T.M., Whitaker J.P., Sparkes R., and Gill P. Analysis and interpretation of mixed forensic stains using DNA STR profiling. Forensic Sci. Int. 91 (1998) 55-70
16. Alessandrini F., Cecati M., Pesaresi M., Turchi C., Carle F., and Tagliabracci A. Fingerprints as evidence for a genetic profile: morphological study on fingerprints and analysis of exogenous and individual factors affecting DNA typing. J. Forensic Sci. 48 (2003) 586-592
17. Lowe A., Murray C., Whitaker J., Tully G., and Gill P. The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces. Forensic Sci. Int. 129 (2002) 25-34
18. Poy A.L., and van Oorschot R.A.H. Trace DNA presence, origin and transfer within a forensic biology laboratory and its potential effect on casework. J. Forensics Identification 56 (2006) 558-576