Cloning, sequencing and antigenic characterization of rVirB9 of Anaplasma marginale isolated from Paraná State, Brazil

Genetics and Molecular Research

Volumn 7, Issue 2, 2008, Pages 460-466

  Vidotto, M C ^a   Venancio E J ^b   Vidotto, O ^a  

^a CCA   (Brazil)

^b STATE UNIVERSITY OF LONDRINA   (Brazil)

Author keywords

Anaplasma marginale; Bovine anaplasmosis; Sequence of VirB9

Indexed keywords

OUTER MEMBRANE PROTEIN;

AMINO ACID SEQUENCE; ANAPLASMA MARGINALE; ANAPLASMOSIS; ANTIGENICITY; ARTICLE; BACTERIAL GENE; BACTERIAL OUTER MEMBRANE; BACTERIUM ISOLATE; BRAZIL; CATTLE; ESCHERICHIA COLI; FUSION GENE; GENE OVEREXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; VACCINE PRODUCTION; VIRB9 GENE;

ANAPLASMA MARGINALE; BACTERIA (MICROORGANISMS); BOS; BOVINAE; ESCHERICHIA COLI; IXODIDA;

EID: 47949103935     PISSN: None     EISSN: 16765680     Source Type: Journal    
DOI: 10.4238/vol7-2gmr416     Document Type: Article

References (18)

1. Alleman AR, Palmer GH, McGuire TC, McElwain TF, et al. (1997). Anaplasma marginale major surface protein 3 is encoded by a polymorphic, multigene family. Infect. Immun. 65: 156-163.
2. Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
3. Censini S, Lange C, Xiang Z, Crabtree JE, et al. (1996). cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc. Natl. Acad. Sci. U. S. A. 93: 14648-14653.
4. Christie PJ, Atmakuri K, Krishnamoorthy V, Jakubowski S, et al. (2005). Biogenesis, architecture, and function of bacterial type IV secretion systems. Annu. Rev. Microbiol. 59: 451-485.
5. Felek S, Huang H and Rikihisa Y (2003). Sequence and expression analysis of virB9 of the type IV secretion system of Ehrlichia canis strains in ticks, dogs, and cultured cells. Infect. Immun. 71: 6063-6067.
6. Foulongne V, Bourg G, Cazevieille C, Michaux-Charachon S, et al. (2000). Identification of Brucella suis genes affecting intracellular survival in an in vitro human macrophage infection model by signature-tagged transposon mutagenesis. Infect. Immun. 68: 1297-1303.
7. Lammertyn E and Anne J (2004). Protein secretion in Legionella pneumophila and its relation to virulence. FEMS Microbiol. Lett. 238: 273-279.
8. Lopez JE, Siems WF, Palmer GH, Brayton KA, et al. (2005). Identification of novel antigenic proteins in a complex Anaplasma marginale outer membrane immunogen by mass spectrometry and genomic mapping. Infect. Immun. 73: 8109-8118.
9. Lopez JE, Palmer GH, Brayton KA, Dark MJ, et al. (2007). Immunogenicity of Anaplasma marginale type IV secretion system proteins in a protective outer membrane vaccine. Infect. Immun. 75: 2333-2342.
10. Niu H, Rikihisa Y, Yamaguchi M and Ohashi N (2006). Differential expression of VirB9 and VirB6 during the life cycle of Anaplasma phagocytophilum in human leucocytes is associated with differential binding and avoidance of lysosome pathway. Cell Microbiol. 8: 523-534.
11. Ohashi N, Zhi N, Lin Q and Rikihisa Y (2002). Characterization and transcriptional analysis of gene clusters for a type IV secretion machinery in human granulocytic and monocytic ehrlichiosis agents. Infect. Immun. 70: 2128-2138.
12. Palmer GH and McElwain TF (1995). Molecular basis for vaccine development against anaplasmosis and babesiosis. Vet. Parasitol. 57: 233-253.
13. Palmer GH, Futse JE, Knowles DP Jr and Brayton KA (2006). Insights into mechanisms of bacterial antigenic variation derived from the complete genome sequence of Anaplasma marginale. Ann. N. Y. Acad. Sci. 1078: 15-25.
14. Richey EJ (1981). Bovine anaplasmosis. In: Current veterinary therapy: food animal practice (Howard RJ, ed.). Saunders, Philadelphia, 767-772.
15. Sambrook J, Fritsch EF and Maniatis T (1989). Molecular Cloning - A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
16. Tebele N, McGuire TC and Palmer GH (1991). Induction of protective immunity by using Anaplasma marginale initial body membranes. Infect. Immun. 59: 3199-3204.
17. Towbin H and Gordon J (1984). Immunoblotting and dot immunobinding- current status and outlook. J. Immunol. Methods 72: 313-340.
18. Vidotto MC, McGuire TC, McElwain TF, Palmer GH, et al. (1994). Intermolecular relationships of major surface proteins of Anaplasma marginale. Infect. Immun. 62: 2940-2946.