Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR

Journal of Virological Methods

Volumn 152, Issue 1-2, 2008, Pages 13-17

  Vandenbussche, F ^a   Vanbinst, T ^a   Vandemeulebroucke, E ^a   Goris, N ^a   Sailleau, C ^b   Zientara, S ^b   De Clercq, K ^a  

^a VETERINARY AND AGROCHEMICAL RESEARCH CENTRE   (Belgium)

^b ECOLE NATIONALE VÉTÉRINAIRE D ALFORT   (France)

Author keywords

Bluetongue virus; Multiplex; Pooling; Real time RT PCR

Indexed keywords

BETA ACTIN; VIRUS RNA;

ANIMAL CELL; ARTICLE; BLUETONGUE ORBIVIRUS; CONTROLLED STUDY; LABORATORY DIAGNOSIS; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS LOAD;

ACTINS; ANIMALS; BLUETONGUE; BLUETONGUE VIRUS; CATTLE; CATTLE DISEASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SHEEP;

ANIMALIA; BLUETONGUE VIRUS; BLUETONGUE VIRUS 8;

EID: 47949092182     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.06.005     Document Type: Article

References (50)

1. Akita G.Y., Glenn J., Castro A.E., and Osburn B.I. Detection of bluetongue virus in clinical samples by polymerase chain reaction. J. Vet. Diagn. Invest. 5 (1993) 154-158
2. ® VIC Probes. At: www.appliedbiosystems.com/techsupport (Consulted March 11, 2008).
3. Aradaib I.E., Smith W.L., Osburn B.I., and Cullor J.S. A multiplex pcr for simultaneous detection and differentiation of north american serotypes of bluetongue and epizootic hemorrhagic disease viruses. Comp. Immunol. Microbiol. Infect. Dis. 26 (2003) 77-87
4. Billinis C., Koumbati M., Spyrou V., Nomikou K., Mangana O., Panagiotidis C.A., and Papadopoulos O. Bluetongue virus diagnosis of clinical cases by a duplex reverse transcription-pcr: a comparison with conventional methods. J. Virol. Methods 98 (2001) 77-89
5. Bréard E., Sailleau C., Hamblin C., and Zientara S. Bluetongue virus in the French Island of Réunion. Vet. Microbiol. 106 (2005) 157-165
6. Calistri P., Giovannini A., Conte A., Nanninni D., Santucci U., Patta C., Rolesu S., and Caporale V. Bluetongue in italy. Part I. Vet. Ital. 40 (2004) 243-251
7. Chamberlain J.S., Gibbs R.A., Rainer J.E., Nguyen P.N., and Thomas C. Deletion screening of the duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acids Res. 16 (1988) 11141-11156
8. Commission Regulation (EC) No 1266/2007 of 26 October 2007 on implementing rules for Council Directive 2000/75/EC as regards the control, monitoring, surveillance and restrictions on movements of certain animals of susceptible species in relation to bluetongue. Official Journal of the European Union L, 283, 37-52.
9. Dangler C.A., de Mattos C.A., de Mattos C.C., and Osburn B.I. Identifying bluetongue virus ribonucleic acid sequences by the polymerase chain reaction. J. Virol. Methods 28 (1990) 281-292
10. Elbers A.R., Backx A., Ekker H.M., van der Spek A.N., and van Rijn P.A. Performance of clinical signs to detect bluetongue virus serotype 8 outbreaks in cattle and sheep during the 2006-epidemic in The Netherlands. Vet. Microbiol. 129 (2008) 156-162
11. Elnifro E.M., Ashshi A.M., Cooper R.J., and Klapper P.E. Multiplex PCR: optimization and application in diagnostic virology. Clin. Microbiol. Rev. 13 (2000) 559-570
12. European Community Reference Laboratory (CRL) for Bluetongue, 2006. Bluetongue virus in The Netherlands identified as serotype 8 by Institute for Animal Health. ProMed. August 28, 2006, accessed at http://www.promedmail.org, archive no. 20060828.2448.
13. Gerdes G.H. A South African overview of the virus, vectors, surveillance and unique features of bluetongue. Vet. Ital. 40 (2004) 39-42
14. Gibbs E.P., and Greiner E.C. The epidemiology of bluetongue. Comp. Immunol. Microbiol. Infect. Dis. 17 (1994) 207-220
15. Goris N., Merkelbach-Peters P., Diev V.I., Verloo D., Zakharov V.M., Kraft H.P., and De Clercq K. European pharmacopoeia foot-and-mouth disease vaccine potency testing in cattle: between test variability and its consequences. Vaccine 25 (2007) 3373-3379
16. Jimenez-Clavero M.A., Aguero M., San Miguel E., Mayoral T., Lopez M.C., Ruano M.J., Romero E., Monaco F., Polci A., Savini G., and Gomez-Tejedor C. High throughput detection of bluetongue virus by a new real-time fluorogenic reverse transcription-polymerase chain reaction: Application on clinical samples from current mediterranean outbreaks. J. Vet. Diagn. Invest. 18 (2006) 7-17
17. Katz J.B., Alstad A.D., Gustafson G.A., and Moser K.M. Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid. J. Clin. Microbiol. 31 (1993) 3028-3030
18. Lager I.A. Bluetongue virus in South America: overview of viruses, vectors, surveillance and unique features. Vet. Ital. 40 (2004) 89-93
19. Le Dimna M., Vrancken R., Koenen F., Bougeard S., Mesplède A., Hutet E., Kuntz-Simon G., and Le Potier M.-F. Validation of two commercial real-time-PCR kits for rapid and specific diagnosis of classical swine fever virus. J. Virol. Methods 147 (2008) 136-142
20. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25 (2001) 402-408
21. Macedo de Oliveira A., Beecham B.D., Montgomery S.P., Lanciotti R.S., Linnen J.M., Giachetti C., Pietrelli L.A., Stramer S.L., and Safranek T.J. West Nile virus blood transfusion-related infection despite nucleic acid testing. Transfusion 44 (2004) 1695-1699
22. MacLachlan N.J. The pathogenesis and immunology of bluetongue virus infection of ruminants. Comp. Immunol. Microbiol. Infect. Dis. 17 (1994) 197-206
23. MacLachlan N.J., and Osburn B.I. Impact of bluetongue virus infection on the international movement and trade of ruminants. J. Am. Vet. Med. Assoc. 228 (2006) 1346-1349
24. McColl K.A., and Gould A.R. Bluetongue virus infection in sheep: haematological changes and detection by polymerase chain reaction. Aust. Vet. J. 71 (1994) 97-101
25. Mellor P.S., Boorman J., and Baylis M. Culicoides biting midges: their role as arbovirus vectors. Annu. Rev. Entomol. 45 (2000) 307-340
26. Méroc E., Faes C., Herr C., Staubach C., Verheyden B., Vanbinst T., Vandenbussche F., Hooyberghs J., Aerts M., De Clercq K., and Mintiens K. Establishing the spread of bluetongue virus at the end of the 2006 epidemic in Belgium. Vet. Microbiol. (2008) 10.1016/j.vetmic.2008.03.012
27. Mertens P.P., Diprose J., Maan S., Singh K.P., Attoui H., and Samuel A.R. Bluetongue virus replication, molecular and structural biology. Vet. Ital. 40 (2004) 426-437
28. Mo C.L., Thompson L.H., Homan E.J., Oviedo M.T., Greiner E.C., Gonzalez J., and Saenz M.R. Bluetongue virus isolations from vectors and ruminants in Central America and the Caribbean. Interamerican bluetongue team. Am. J. Vet. Res. 55 (1994) 211-215
29. Munoz-Zanzi C.A., Johnson W.O., Thurmond M.C., and Hietala S.K. Pooled-sample testing as a herd-screening tool for detection of bovine viral diarrhea virus persistently infected cattle. J. Vet. Diagn. Invest. 12 (2000) 195-203
30. Pennings J.L., Van De Locht L.T., Jansen J.H., Van der Reijden B.A., De Witte T., and Mensink E.J. Degradable dU-based DNA template as a standard in real-time PCR quantitation. Leukemia 15 (2001) 1962-1965
31. Purse B.V., Mellor P.S., Rogers D.J., Samuel A.R., Mertens P.P., and Baylis M. Climate change and the recent emergence of bluetongue in europe. Nat. Rev. Microbiol. 3 (2005) 171-181
32. Raboud J.M., Major C., Sherlock C.H., and O'Shaughnessy M.V. The effects of pooling serum samples from seroconverting individuals or individuals with end stage disease for HIV antibody testing: a comparison of four screen tests and three pool sizes. Serodiagn. Immunother. Infect. Dis. 8 (1996) 19-24
33. Rovira A., Clement T., Christopher-Hennings J., Thompson B., Engle M., Reicks D., and Muñoz-Zanzi C. Evaluation of the sensitivity of reverse-transcription polymerase chain reaction to detect porcine reproductive and respiratory syndrome virus on individual and pooled samples from boars. J. Vet. Diagn. Invest. 19 (2007) 502-509
34. Saldanha J., and Minor P. A sensitive PCR method for detecting HCV RNA in plasma pools, blood products, and single donations. J. Med. Virol. 43 (1994) 72-76
35. Shaw A.E., Monaghan P., Alpar H.O., Anthony S., Darpel K.E., Batten C.A., Guercio A., Alimena G., Vitale M., Bankowska K., Carpenter S., Jones H., Oura C.A., King D.P., Elliott H., Mellor P.S., and Mertens P.P. Development and initial evaluation of a real-time RT-PCR assay to detect bluetongue virus genome segment 1. J. Virol. Methods 145 (2007) 115-126
36. Singer R.S., MacLachlan N.J., and Carpenter T.E. Maximal predicted duration of viremia in bluetongue virus-infected cattle. J. Vet. Diagn. Invest. 13 (2001) 43-49
37. Stephens G.M., Raboud J.M., Karakas L., and Sherlock C.H. Can pooling be used for seroprevalence studies of hepatitis C?. J. Clin. Microbiol. 38 (2000) 4264-4265
38. Tabachnick W.J. Culicoides and the global epidemiology of bluetongue virus infection. Vet. Ital. 40 (2004) 145-150
39. Thiry E., Saegerman C., Guyot H., Kirten P., Losson B., Rollin F., Bodmer M., Czaplicki G., Toussaint J.F., De Clercq K., Dochy J.M., Dufey J., Gilleman J.L., and Messeman K. Bluetongue in northern Europe. Vet. Rec. 159 (2006) 327
40. Toussaint J.F., Kerkhofs P., and De Clercq K. Influence des changements climatiques globaux sur la progression des arboviroses. Ann. Med. Vet. 150 (2006) 56-63
41. Toussaint J.F., Vandenbussche F., Mast J., De Meester L., Goris N., Van Dessel W., Vanopdenbosche E., Kerkhofs P., Zientara S., Sailleau C., Czaplicki G., Depoorter G., Dochy J.M., and De Clercq K. Bluetongue in northern Europe. Vet. Rec. 159 (2006) 327
42. Toussaint J.F., Sailleau C., Bréard E., Zientara S., and De Clercq K. Bluetongue virus detection by two real-time rt-qpcrs targeting two different genomic segments. J. Virol. Methods 140 (2007) 115-123
43. Toussaint J.F., Sailleau C., Mast J., Houdart P., Czaplicki G., Demeestere L., VandenBussche F., Van Dessel W., Goris N., Bréard E., Bounaadja L., Etienne T., Zientara S., and De Clercq K. Bluetongue in Belgium, 2006. Emerg. Infect. Dis. 13 (2007) 614-616
44. Vandenbussche F., Vanbinst T., Verheyden B., Van Dessel W., Demeestere L., Houdart P., Bertels G., Praet N., Berkvens D., Mintiens K., Goris N., and De Clercq K. Evaluation of antibody-ELISA and real-time RT-PCR for the diagnosis and profiling of bluetongue virus serotype 8 during the epidemic in Belgium in 2006. Vet. Microbiol. 129 (2008) 15-27
45. Verwoerd D.E. Bluetongue. In: Tustin R.C., and Coetzer J.A. (Eds). Infectious Diseases Of Livestock (2004), Oxford University Press, Cape Town 1201-1220
46. Wade-Evans A.M., Mertens P.P., and Bostock C.J. Development of the polymerase chain reaction for the detection of bluetongue virus in tissue samples. J. Virol. Methods 30 (1990) 15-24
47. Walton T.E. The history of bluetongue and a current global overview. Vet. Ital. 40 (2004) 31-38
48. Wilson A., Carpenter S., Gloster J., and Mellor P. Re-emergence of bluetongue in northern Europe in 2007. Vet. Rec. 161 (2007) 487-489
49. Wilson W.C., and Chase C.C. Nested and multiplex polymerase chain reactions for the identification of bluetongue virus infection in the biting midge, Culicoides variipennis. J. Virol. Methods 45 (1993) 39-47
50. Zientara S., Sailleau C., Dauphin G., Roquier C., Remond E.M., Lebreton F., Hammoumi S., Dubois E., Agier C., Merle G., and Bréard E. Identification of bluetongue virus serotype 2 (Corsican strain) by reverse-transcriptase PCR reaction analysis of segment 2 of the genome. Vet. Rec. 150 (2002) 598-601