Sequential CaCl2, polyethylene glycol precipitation for RNase-free plasmid DNA isolation

Analytical Biochemistry

Volumn 380, Issue 2, 2008, Pages 310-314

  Sauer, Marie Laure ^a   Kollars, Brett ^a   Geraets, Ryan ^a   Sutton, Fedora ^a  

^a SOUTH DAKOTA STATE UNIVERSITY   (United States)

Author keywords

CaCl2; Plasmid DNA purification; Polyethylene glycol (PEG); RNA; RNase free

Indexed keywords

ALIPHATIC COMPOUNDS; BIOMOLECULES; ELECTROPHORESIS; POLYETHYLENE GLYCOLS; POLYETHYLENES; POLYMERASE CHAIN REACTION; PURIFICATION; RNA; SODIUM CHLORIDE; TRANSCRIPTION;

CACL2; FUNCTIONAL GENOMICS; GEL ELECTROPHORESIS; HETEROLOGOUS SYSTEMS; PLASMID DNA; PLASMID PURIFICATION; RNASE FREE; SELECTIVE PRECIPITATION;

DNA;

2 PROPANOL; CALCIUM CHLORIDE; KANAMYCIN; MACROGOL; PLASMID DNA; RIBONUCLEASE; RNA; SODIUM CHLORIDE; DNA; MACROGOL DERIVATIVE;

ARTICLE; CULTURE MEDIUM; DNA PURIFICATION; ESCHERICHIA COLI; FUNCTIONAL GENOMICS; GENE EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRECIPITATION; PRIORITY JOURNAL; RECOMBINANT PLASMID; SPECTROPHOTOMETER; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION;

BASE SEQUENCE; CALCIUM CHLORIDE; DNA; ESCHERICHIA COLI; PLASMIDS; POLYETHYLENE GLYCOLS; PRECIPITATION; RIBONUCLEASES; RNA; SODIUM CHLORIDE;

EID: 47749146447     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.05.044     Document Type: Article

References (14)

1. Kachel V., Sindelar G., and Grimm S. High-throughput isolation of ultra-pure plasmid DNA by a robotic system. BMC Biotechnol. 6 (2006) 9
2. Zhang H., Chen H.-T., and Glisin V. Isolation of DNA-free RNA, DNA, and proteins by cesium trifluoroacetate centrifugation. Biochem. Biophys. Res. Commun. 312 (2003) 131-137
3. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
4. Lis J.T. Fractionation of DNA fragments by polyethylene glycol induced precipitation. Methods Enzymol. 65 (1980) 347-353
5. Lis J.T., and Schleif R. Size fractionation of double-stranded DNA by precipitation with polyethylene glycol. Nucleic Acids Res. 2 (1975) 383-389
6. J.L. Hartley, H. Bowen, PEG precipitation for selective removal of small DNA fragments, [online] downloaded 15/01/08, http://www.invitrogen.co.jp/focus/181027.pdf.
7. Schmitz A., and Riesner D. Purification of nucleic acids by selective precipitation with polyethylene glycol 6000. Anal. Biochem. 354 (2006) 311-313
8. Stepanov V.G., and Nyborg J. Preparative purification of plasmid DNA templates for in vitro transcription assays by consecutive differential precipitations. J. Biotechnol. 102 (2003) 223-231
9. Murphy J.C., Wibbenmeyer J.A., Fox G.E., and Willson R.C. Purification of plasmid DNA using selective precipitation by compaction agents. Nat. Biotechnol. 17 (1999) 822-823
10. Murphy J.C., Fox G.E., and Willson R.C. RNA isolation and fractionation with compaction agents. Anal. Biochem. 295 (2001) 143-148
11. Auffray C., and Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur. J. Biochem. 107 (1980) 303-314
12. Eon-Duval A., Gumbs K., and Ellett C. Precipitation of RNA impurities with high salt in a plasmid DNA purification process: use of experimental design to determine reaction conditions. Biotechnol. Bioeng. 83 (2003) 544-553
13. Cerutti H., Johnson A.M., Gillham N.W., and Boynton J.E. A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression. Genetics 145 (1997) 97-110
14. Lübbert H., Hoffman B.J., Snutch T.P., van Dyke T., Levine A.J., Hartig P.R., Lester H.A., and Davidson N. cDNA cloning of a serotonin 5-HT1C receptor by electrophysiological assays of mRNA-injected Xenopus oocytes. Proc. Natl. Acad. Sci. USA 84 (1987) 4332-4336