Using the eosin-5-maleimide binding test in the differential diagnosis of hereditary spherocytosis and hereditary pyropoikilocytosis

Cytometry Part B - Clinical Cytometry

Volumn 74, Issue 4, 2008, Pages 244-250

  King, May Jean ^a   Telfer, Paul ^b   MacKinnon, Heather ^c   Langabeer, Lisa ^b,d   McMahon, Corrina ^d   Darbyshire, Philip ^e   Dhermy, Didier ^f  

^a NHS BLOOD AND TRANSPLANT   (United Kingdom)

^b QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^c WHITTINGTON HOSPITAL NHS TRUST   (United Kingdom)

^d OUR LADY S CHILDREN S HOSPITAL   (Ireland)

^e Hospital for Sick Children   (United Kingdom)

^f INSTITUT NATIONAL DE LA TRANSFUSION SANGUINE   (France)

Author keywords

Eosin 5 maleimide; Flow cytometry; Hereditary pyropoikilocytosis; Hereditary spherocytosis

Indexed keywords

EOSIN; EOSIN 5 MALEIMIDE; MALEIMIDE DERIVATIVE; MEMBRANE PROTEIN; SPECTRIN;

ADOLESCENT; ADULT; AGED; ARTICLE; CELL LABELING; CELL SUBPOPULATION; CELL VOLUME; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; DIFFERENTIAL DIAGNOSIS; ERYTHROCYTE DISORDER; FEMALE; FLOW CYTOMETRY; FLUORESCENCE; HEREDITARY PYROPOIKILOCYTOSIS; HEREDITARY SPHEROCYTOSIS; HUMAN; HUMAN CELL; INFANT; MALE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRESCHOOL CHILD; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN VARIANT; SCHOOL CHILD; SCREENING TEST;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANEMIA, HEMOLYTIC, CONGENITAL; CHILD; CHILD, PRESCHOOL; DIAGNOSIS, DIFFERENTIAL; EOSINE YELLOWISH-(YS); ERYTHROCYTE MEMBRANE; ERYTHROCYTES, ABNORMAL; FEMALE; FLOW CYTOMETRY; HUMANS; INFANT; MALE; MIDDLE AGED; SPECTRIN; SPHEROCYTOSIS, HEREDITARY;

EID: 47549102275     PISSN: 15524949     EISSN: 15524957     Source Type: Journal    
DOI: 10.1002/cyto.b.20413     Document Type: Article

References (20)

1. King M-J, Behrens J, Rogers C, Flynn C, Greenwood D, Chambers K. Rapid flow cytometric test for the diagnosis of membrane cytoskeleton-associated haemolytic anaemia. Br J Haematol 2000;111:924-933.
2. King M-J, Smythe J, Mushens R. Eosin-5-maleimide binding to band 3 and Rh-related proteins forms the basis of a screening test for hereditary spherocytosis. Br J Haematol 2004;124:106-113.
3. Palek J, Jarolim P. Clinical expression and laboratory detection of red blood cell membrane protein mutations. Sem Hematol 1993;30:250-275.
4. Coetzer T, Lawler J, Prchal JT, Palek J. Molecular determinants of clinical expression of hereditary elliptocytosis and pyropokilocytosis. Blood 1987;70:766-772.
5. Coetzer T, Palek J, Lawler J, Liu SC, Jarolim P, Lahav M, Prchal JT, Wang W. Alter BP, Schewitz G, Mankad V, Gallanello R, Cao A. Structural and functional heterogeneity of α spectrin mutations involving the spectrin heterodimer self-associations site: Relationships to haematologic expression of homozygous hereditary elliptocytosis and hereditary pyropoikilocytosis. Blood 1990;75:2235-2244.
6. Dhermy D, Garbarz M, Lecomte MC, Féo C, Bournier O, Chaveroche I, Gautero H, Galand C, Boivin P. Hereditary elliptocytosis: Clinical, morphological and biochemical studies of 38 cases. Nouv Rev Franç d'Hématologie 1986;28:129-140.
7. Lecomte MC, Garbarz M, Gautero H, Bournier G, Galand C, Boivin P, Dhermy D. Molecular basis of clinical and morphological heterogeneity in hereditary elliptocytosis (HE) with spectrin αI variants. Br J Haematol 1993;85:584-595.
8. Alloisio N, Morlé L, Maréchal J, Roux A-F, Ducluzeau M-T, Guetarni D, Pothier B, Baklouti F, Ghanem A, Kastally R, Delaunay J. SpαV/41: A common spectrin polymorphism at the αIV-αV domain junction. Relevance to the expression level of hereditary elliptocytosis due to α due to α-spectrin variants located in trans. J Clin Invest 1991;87:2169-2177.
9. Langlois RG, Bigbee WL, Jenson RH. Flow cytometric characterization of normal and variant cells with monoclonal antibodies specific for glycophorin A. J Immunol 1985;134:4009-4017.
10. null human erythrocytes. Biochem J 1988;251:499-505.
11. Gardner B, Anstee DJ, Mawby WJ, Tanner MJA, von dem Borne AEGKr. The abundance and organization of polypeptides associated with antigens of the Rh blood group system. Transf Med 1991;1:77-85.
12. Smythe J, Spring F, Gardner B, Parsons SF, Judson PA, Anstee DJ. Monoclonal antibodies recognising epitopes on the extracellular face and intracellular N-terminus of the human erythrocyte anion transporter (Band 3) and their application to the analysis of South East Asian ovalocytes. Blood 1995;85:2929-2936.
13. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685.
14. Fairbanks G, Steck TL, Wallach DFH. Electrophoresis analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 1971;10:2606-2617.
15. Liu S-C, Palek J, Prchal JT. Defective spectrin dimer-dimer association in hereditary elliptocytosis. Proc Natl Acad Sci USA 1982;79:2072-2076.
16. Morrow JS, Speicher DW, Knowles WJ, Hsu CJ, Marchesi VT. Identification of functional domains of human erythrocyte spectrin. Proc Natl Acad Sci USA 1980;77:6592-6596.
17. Coetzer T, Lawler J, Liu S-C, Prchal JT, Gaultieri R, Brain MC, Dacie JV, Palek J. Partial ankyrin and spectrin deficiency in severe atypical hereditary spherocytosis. N Eng J Med 1988;318:230-234.
18. Kedar PS, Colah RB, Kulkarni S, Ghosh K, Mohanty D. Experience with eosin-5-maleimide as a diagnostic tool for red cell membrane cytoskeleton disorders. Clin Lab Haematol 2003;25:373-376.
19. Stoya G, Gruhn B, Vogelsang H, Baumann E, Linss W. Flow cytometry as a diagnostic tool for hereditary spherocytosis. Acta Haematologica 2006;116:186-191.
20. Girodon F, Garçon L, Bergoin E, Largier M, Delaunay J, Feneant-Thibault M, Maynadié M, Couillaud G, Moreira S, Cynober T. Usefulness of the eosin-5-maleimide cytometric method as a first-line screening test for the diagnosis of hereditary spherocytosis: Comparison with ektacytometry and protein electrophoresis. Br J Haematol 2008;140:468-470.