Effects of a novel disulfide bond and engineered electrostatic interactions on the thermostability of azurin

Biochemistry

Volumn 43, Issue 39, 2004, Pages 12563-12574

  Tigerström, Anna ^a   Schwarz, Frederick ^b   Karlsson, Göran ^c   Ökvist, Mats ^a   Alvarez Rúa, Carmen ^d   Maeder, Dennis ^e   Robb, Frank T ^e   Sjölin, Lennart ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

^b NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^c CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

^d UNIVERSITY OF OVIEDO   (Spain)

^e UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL BONDS; COPPER; CRYSTAL STRUCTURE; SPECTROSCOPY; THERMAL EFFECTS; THERMODYNAMIC STABILITY;

AZURIN; DISULPHIDE BONDS; ELECTROSTATIC INTERACTIONS; MUTANTS;

PROTEINS;

AZURIN; MUTANT PROTEIN;

ALPHA HELIX; ARTICLE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; DIFFERENTIAL SCANNING CALORIMETRY; DISULFIDE BOND; ELECTRICITY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN MODIFICATION; PSEUDOMONAS AERUGINOSA; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

ARGININE; ASPARTIC ACID; AZURIN; CALORIMETRY, DIFFERENTIAL SCANNING; COPPER; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; DISULFIDES; ELECTROSTATICS; LYSINE; MASS SPECTROMETRY; MUTAGENESIS, SITE-DIRECTED; PROTEIN DENATURATION; PSEUDOMONAS AERUGINOSA; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROPHOTOMETRY; THERMODYNAMICS; ZINC;

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 4744372269     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi048926x     Document Type: Article

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