Functional expression of recombinant human ribonuclease/angiogenin inhibitor in stably transformed Drosophila melanogaster S2 cells

Cytotechnology

Volumn 57, Issue 1, 2008, Pages 93-99

  Park, Jong Hwa ^a   Hwang, In Sook ^b   Kim, Kyung Il ^b   Lee, Jong Min ^b   Park, Young Min ^c   Park, Chang Ho ^b   Chung, In Sik ^b  

^a Konkuk University   (South Korea)

^b Kyung Hee University   (South Korea)

^c Yeojoo Institute of Technology   (South Korea)

Author keywords

Drosophila melanogaster S2 cells; Purification; RAI; Ribonuclease inhibitor activity; Ribonuclease angiogenin inhibitor

Indexed keywords

RIBONUCLEASE A; RIBONUCLEASE ANGIOGENIN INHIBITOR; RIBONUCLEASE INHIBITOR; RNA; TRANSFER RNA;

ANIMAL CELL; ARTICLE; CELL FRACTIONATION; CELL GROWTH; CELL POPULATION; CONTROLLED STUDY; DROSOPHILA; ENZYME ACTIVITY; ENZYME INHIBITION; HUMAN; INSECT CELL CULTURE; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; RNA DEGRADATION; YEAST;

DROSOPHILA MELANOGASTER; HEXAPODA;

EID: 47349118643     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-008-9126-3     Document Type: Article

References (17)

1. Barnett BB (1998) Insect cell culture technology: research & production development, Hyclone. Art To Sci 17:1-7
2. Blackburn P (1979) Ribonuclease inhibitor from human placenta: rapid purification and assay. J Biol Chem 254:12484-12487
3. Chang KH, Park JH, Lee YH, Kim JH, Chun HO, Kim JH, Chung IS (2002) Dimethylsulfoxide and sodium butyrate enhance the production of recombinant cyclooxygenase 2 in stably transformed Drosophila melanogaster S2 cells. Biotechnol Lett 24:1353-1359
4. Courey AJ, Tjian R (1988) Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif. Cell 55:887-898
5. Frank S, Vallee BL (1989) Expression of human placental ribonuclease inhibitor in Escherichia coli. Biochem Biophys Res Commun 160:115-120
6. Jeon HK, Chang KH, Kim KI, Chung IS (2003) Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells. Biotechnol Lett 25:185-189
7. Johansen H, Van Der Straten A, Sweet R, Otto E, Maroni G, Rosenberg M (1989) Regulated expression at high copy number allows production of a growth-inhibitory oncogene product in Drosophila Schneider cells. Genes Dev 3:882-889
8. Kerbel RS (1997) A cancer therapy to resistance. Nature 390:335-336
9. Klink TA, Vicentini AM, Hofsteenge J, Raines RT (2001) High-level soluble production and characterization of porcine ribonuclease inhibitor. Protein Expr Purif 22:174-179
10. Laemmli UK (1970) Cleavage of structural proteins during the assembly of head of bacteriophage. Nature 227:680-685
11. Lee FS, Shapiro R, Vallee BL (1989) Tight-binding inhibition of angiogenin and ribonuclease A by placental ribonuclease inhibitor. Biochemistry 28:225-230
12. Lee FS, Vallee BL (1994) Structure and action of mammalian ribonuclease (angiogenin) inhibitor. Prog Nucleic Acid Res Mol Biol 44:1-30
13. Moenner M, Vosoghi M, Ryazantsev S, Glitz DG (1998) Ribonuclease inhibitor protein of human erythrocytes: characterization, loss of activity in response to oxidative stress, and association with Heinz bodies. Blood Cells Mol Dis 24:149-164
14. Nadano D, Yasuda T, Takeshita H, Uchide K, Kishi K (1994) Purification and characterization of human brain ribonuclease inhibitor. Arch Biochem Biophys 312:421-428
15. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory mannual. Cold Spring Harbour Laboratory Press, New York
16. Schneider I (1972) Cell lines derived from late embryonic stages of Drosophila melanogaster. J Embryol Exp Morphol 27:353-365
17. Shapiro R, Vallee BL (1987) Human placental ribonuclease inhibitor abolishes both angiogenic and ribonucleic activities of angiogenin. Proc Natl Aca Sci 84:2238-2241