Sodium hypochlorite denatures the DNA of the amphibian chytrid fungus Batrachochytrium dendrobatidis

Diseases of Aquatic Organisms

Volumn 80, Issue 1, 2008, Pages 63-67

  Cashins, Scott D ^a   Skerratt, Lee F ^a   Alford, Ross A ^a  

^a JAMES COOK UNIVERSITY   (Australia)

Author keywords

Chytridiomycosis; Denaturation; Diagnostic test; Field hygiene; PCR

Indexed keywords

DISINFECTANT AGENT; FUNGAL DNA; HYPOCHLORITE SODIUM;

BIOASSAY; CHLORITE; DNA; FUNGUS; POLYMERASE CHAIN REACTION; REAL TIME; SODIUM;

AMPHIBIA; ANIMAL; ANIMAL DISEASE; ARTICLE; CHYTRIDIOMYCETES; DNA DENATURATION; DRUG EFFECT; LIFE CYCLE; MICROBIOLOGY; MYCOSIS; POLYMERASE CHAIN REACTION;

AMPHIBIANS; ANIMALS; CHYTRIDIOMYCOTA; DISINFECTANTS; DNA, FUNGAL; LIFE CYCLE STAGES; MYCOSES; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; SODIUM HYPOCHLORITE;

AMPHIBIA; BATRACHOCHYTRIUM DENDROBATIDIS; FUNGI;

EID: 47349110360     PISSN: 01775103     EISSN: 16161580     Source Type: Journal    
DOI: 10.3354/dao01919     Document Type: Article

References (24)

1. Annis SL, Dastoor FP, Ziel H, Daszak P, Longcore JE (2004) A DNA-based assay identifies Batrachochytrium dendrobatidis in amphibians. J Wildl Dis 40:420-428
2. Berger L, Speare R, Daszak P, Green DE and others (1998) Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America. Proc Natl Acad Sci USA 95: 9031-9036
3. Boyle DG, Hyatt AD, Daszak P, Berger L and others (2003) Cryo-archiving of Batrachochytrium dendrobatidis and other chytridiomycetes. Dis Aquat Org 56:59-64
4. Boyle DG, Boyle DB, Olsen V, Morgan JAT, Hyatt AD (2004) Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time TaqMan PCR assay. Dis Aquat Org 60:141-148
5. Clarkson RM, Moule AJ (1998) Sodium hypochlorite and its use as an endodontic irrigant. Aust Dent J 43:250-256
6. Eventov VL, Andrianova MY, Kukaeva EA (1998) Detoxification and disinfection with sodium hypochlorite. Biomed Eng (NY) 32:349-352
7. Gomes BPFA, Ferraz CCR, Vianna ME, Berber VB, Teixeira FB, Souza-Filho FJ (2001) In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of Enterococcus faecalis. Int Endod J 34:424-428
8. Hyatt AD, Boyle DG, Olsen V, Boyle DB and others (2007) Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis. Dis Aquat Org 73: 175-192
9. Johnson ML, Berger L, Phillips L, Speare R (2003) Fungicidal effects of chemical disinfectants, UV light, desiccation and heat on the amphibian chytrid Batrachochytrium dendrobatidis. Dis Aquat Org 57:255-260
10. Kemp BM, Smith DG (2005) Use of bleach to eliminate contaminating DNA from the surface of bones and teeth. Forensic Sci Int 154:53-61
11. Kirshtein JD, Anderson CW, Wood JS, Longcore JE, Voytek MA (2007) Quantitative PCR detection of Batrachochytrium dendrobatidis DNA from sediments and water. Dis Aquat Org 77:11-15
12. LeChevallier MW, Cawthon CD, Lee RG (1988) Factors promoting survival of bacteria in chlorinated water supplies. Appl Environ Microbiol 54:649-654
13. Lips KR, Brem F, Brenes R, Reeve JD and others (2006) Emerging infectious disease and the loss of biodiversity in a Neotropical amphibian community. Proc Natl Acad Sci USA 103:3165-3170
14. Martyny J, Harbeck R, Pacheco K, Barker E and others (2005) Aerosolized sodium hypochlorite inhibits viability and allergenicity of mold on building materials. J Allergy Clin Immunol 116:630-635
15. Matsui E, Kagey-Sobotka A, Chichester K, Eggleston PA (2003) Allergic potency of recombinant Fel d 1 is reduced by low concentrations of chlorine bleach. J Allergy Clin Immunol 111:396-401
16. Ohnishi S, Murata M, Kawanishi S (2002) DNA damage induced by hypochlorite and hypobromite with reference to inflammation-associated carcinogenesis. Cancer Lett 178:37-42
17. Pessier AP, Nichols DK, Longcore JE, Fuller MS (1999) Cutaneous chytridiomycosis in poison dart frogs (Dendrobates spp.) and White's tree frogs (Litoria caerulea). J Vet Diagn Invest 11:194-199
18. Prince AM, Andrus L (1992) PCR: how to kill unwanted DNA. Biotechniques 12:37-42
19. Rachowicz LJ, Knapp RA, Morgan JAT, Stice MJ, Vreden-burg VT, Parker JM, Briggs CJ (2006) Emerging infectious disease as a proximate cause of amphibian mass mortality. Ecology 87:1671-1683
20. Sehulster LM, Hollinger FB, Dreesman GR, Melnick JL (1981) Immunological and biophysical alteration of hepatitis B virus antigens by sodium hypochlorite disinfection. Appl Environ Microbiol 42:762-767
21. Skerratt LF, Berger L, Speare R, Cashins S and others (2007) Spread of chytridiomycosis has caused the rapid global decline and extinction of frogs. EcoHealth 4:125-134
22. Speare R, Berger L, Skerratt LF, Alford R and others (2004) Hygiene protocol for handling amphibians in field studies. Available at: www.jcu.edu.au/school/phtm/PHTM/frogs/field-hygiene.pdf
23. Webb R, Mendez D, Berger L, Speare R (2007) Additional disinfectants effective against the amphibian chytrid fungus Batrachochytrium dendrobatidis. Dis Aquat Org 74:13-16
24. Woodhams DC, Vredenburg VT, Simon MA, Billheimer D and others (2007) Symbiotic bacteria contribute to innate immune defenses of the threatened mountain yellow-legged frog, Rana muscosa. Biol Conserv 138:390-398