Evaluation of a novel luciferase reporter construct: A positive control plasmid for reporter gene assay

African Journal of Biotechnology

Volumn 7, Issue 13, 2008, Pages 2124-2127

  Tencomnao, Tewin ^a   Rakkhitawatthana, Varaporn ^a   Sukhontasing, Kanya ^a  

^a CHULALONGKORN UNIVERSITY   (Thailand)

Author keywords

Cytomegalovirus promoter enhancer; Human melanoma cell line; Luciferase assay; Reporter gene plasmid

Indexed keywords

LUCIFERASE; VIRUS VECTOR;

ARTICLE; CANCER CELL CULTURE; CYTOMEGALOVIRUS; GENE CONSTRUCT; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; REPORTER GENE;

CYTOMEGALOVIRUS;

EID: 47149100392     PISSN: None     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Alam J, Cook JL (1990). Reporter genes: Application to the study of mammalian gene transcription. Anal. Biochem. 188: 245-254.
2. Boshart M, Weber F, Jahn G, Dorsch Häsler K, Fleckenstein B, Schaffner W (1985). A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell 41: 521-530.
3. Boulin T, Etchberger JF, Hobert O (2006). Reporter gene fusions. In WormBook (ed) The C. elegans Research Community, pp 1-23, www.wormbook.org.
4. Bronstein I, Fortin J, Stanley PE, Stewart GS, Kricka LJ (1994). Chemiluminescent and bioluminescent reporter gene assays. Anal. Biochem. 219: 169-181.
5. Doll RF, Crandal JE, Dyaer CA, Aucoin JM, Smith Fl (1996). Comparison of promoter strength on gene delivery into mammalian brain cells using AAV vectors. Gene Ther. 3: 437-447.
6. Feng G, Hicks P, Chang PL (2003). Differential expression of mammalian or viral promoter-driven gene in adherent versus suspension cells. In Vitro Cell Dev. Biol. Anim., 39: 420-423.
7. Garmory HS, Brown KA, Titball RW (2003). DNA vaccines: improving expression of antigens. Genet. Vaccines Ther. 1: 2.
8. Gould SJ, Subramani S (1988). Firefly luciferase as a tool in molecular and cell biology. Anal. Biochem. 175: 5-13.
9. Lee AH, Suh YS, Sung JH, Yang SH, Sung YC (1997). Comparison of various expression plasmids for the induction of immune response by DNA immunization. Mol. Cells 7: 495-501.
10. Lin MT, Wang F, Uitto J, Yoon K (2001). Differential expression of tissue-specific promoters by gene gun. Br. J. Dermatol. 144: 34-39.
11. Naylor LH (1999). Reporter gene technology: the future looks bright. Biochem. Pharmacol. 58: 749-757.
12. Prasansuklab A (2006). Role of genetic polymorphism in regulating the expression of serotonin transporter. MSc thesis, Chulalongkorn University, Bangkok, Thailand.
13. Schmidt EV, Christoph G, Zeller R, Leder P (1990). The cytomegalovirus enhancer: A pan-active control element in transgenic mice. Mol. Cell. Biol. 10:4406-11.
14. Serganova I, Ponomarev V, Blasberg R (2007). Human reporter genes: potential use in clinical studies. Nucl. Med. Biol. 34: 791-807.
15. Sherf BA, Wood KV (1994). Firefly Luciferase Engineered for Improved Genetic Reporting. Promega Notes Magazine 49: 14.
16. Tencomnao T, Yu RK, Kapitonov D (2001). Characterization of the human UDP-galactose:ceramide galactosyltransferase gene promoter. Biochim. Biophys. Acta. 1517: 416-23.
17. Tencomnao T, Kapitonov D, Bieberich E, Yu RK (2004). Transcriptional regulation of the human UDP-galactose:ceramide galactosyltransferase (hCGT) gene expression: functional role of GC-box and CRE. Glycoconj. J. 20: 339-51.
18. Tucker C, Endo M, Hirono I, Aoki T (2000). Assessment of DNA vaccine potential for juvenile Japanese flounder Paralichthys olivaceus, through the introduction of reporter genes by particle bombardment and histopathology. Vaccine 19: 801-9.
19. Xu ZL, Mizuguchi H, Ishii-Watabe A, Uchida E, Mayumi T, Hayakawa T (2001). Optimization of transcriptional regulatory elements for constructing plasmid vectors. Gene 272: 149-56.