Significantly improved accuracy of diagnosis of early lyme disease by peptide enzyme-linked immunosorbent assay based on the borreliacidal antibody epitope of Borrelia burgdorferi OspC

Clinical and Vaccine Immunology

Volumn 15, Issue 6, 2008, Pages 981-985

  Jobe, Dean A ^a   Lovrich, Steven D ^a   Asp, Krista E ^a   Mathiason, Michelle A ^a   Albrecht, Stephanie E ^a   Schell, Ronald F ^b   Callister, Steven M ^a  

^a GUNDERSEN LUTHERAN MEDICAL CENTER   (United States)

^b WISCONSIN STATE LABORATORY OF HYGIENE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; OUTER SURFACE PROTEIN C7; SYNTHETIC PEPTIDE;

ANTIBODY TITER; ANTIGEN ANTIBODY REACTION; ARTICLE; BORRELIA BURGDORFERI; CONTROLLED STUDY; CROSS REACTION; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMAN; INTERMETHOD COMPARISON; LYME DISEASE; MAJOR CLINICAL STUDY; OPTICAL DENSITY; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; WESTERN BLOTTING;

ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; BORRELIA BURGDORFERI; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; LYME DISEASE; SENSITIVITY AND SPECIFICITY;

EID: 47049114044     PISSN: 15566811     EISSN: 1556679X     Source Type: Journal    
DOI: 10.1128/CVI.00079-08     Document Type: Article

References (33)

1. Anderson, J. F., R. A. Flavell, L. A. Magnarelli, S. W. Barthold, S. S. Kantor, R. Wallich, D. H. Persing, D. Mathiesen, and E. Fikrig. 1996. Novel Borrelia burgdorferi isolates from Ixodes scapularis and Ixodes dentatus ticks feeding on humans. J. Clin. Microbiol. 34:524-529.
2. Bacon, R. M., B. J. Bickerstaff, M. E. Schriefer, R. D. Gilmore, Jr., M. T. Philipp, A. C. Steere, G. P. Wormser, A. R. Marques, and B. J. B. Johnson. 2003. Serodiagnosis of Lyme disease by kinetic enzyme-linked immunosorbent assay using recombinant VlsE1 or peptide antigens of Borrelia burgdorferi compared with 2-tiered testing using whole-cell lysates. J. Infect. Dis. 187:1187-1199.
3. Callister, S. M., D. A. Jobe, W. A. Agger, R. F. Schell, T. J. Kowalski, S. D. Lovrich, and J. A. Marks. 2002. Ability of the borreliacidal antibody test to confirm Lyme disease in clinical practice. Clin. Diagn. Lab. Immunol. 9:908-912.
4. Callister, S. M., D. A. Jobe, R. F. Schell, C. S. Pavia, and S. D. Lovrich. 1996. Sensitivity and specificity of the borreliacidal-antibody test during early Lyme disease: a gold standard? Clin. Diagn. Lab. Immunol. 3:399-402.
5. Callister, S. M., R. F. Schell, K. L. Case, S. D. Lovrich, and S. P. Day. 1993. Characterization of the borreliacidal antibody response to Borrelia burgdorferi in humans: a serodiagnostic test. J. Infect. Dis. 167:158-164.
6. Callister, S. M., R. F. Schell, L. C. L. Lim, D. A. Jobe, K. L. Case, G. L. Bryant, and P. E. Molling. 1994. Detection of borreliacidal antibodies by flow cytometry: an accurate, highly specific serodiagnostic test for Lyme disease. Arch. Intern. Med. 154:1625-1632.
7. Centers for Disease Control and Prevention. 1990. Case definitions for public health surveillance. MMWR Morb. Mortal. Wkly. Rep. 39:19-21.
8. Centers for Disease Control and Prevention. 1995. Recommendations for test performance and interpretations from the Second National Conference on Serologic Diagnosis of Lyme Disease. MMWR Morb. Mortal. Wkly. Rep. 44:590-591.
9. de Silva, A. M., S. R. Telford III, L. R. Brunet, S. W. Barthold, and E. Fikrig. 1996. Borrelia burgdorferi OspA is an arthropod-specific transmission-blocking Lyme disease vaccine. J. Exp. Med. 83:271-275.
10. Fields, C. G., D. H. Lloyd, R. L. MacDonald, K. M. Otteson, and R. L. Noble. 1991. HBTU activation for automated Fmoc solid-phase peptide synthesis. Peptide Res. 4:95-101.
11. Grimm, D., K. Tilly, R. Byram, P. E. Stewart, J. G. Krum, D. M. Bueschel, T. G. Schwan, P. F. Policastro, A. F. Elias, and P. A. Rosa. 2004. Outer-surface protein C of the Lyme disease spirochete: a protein induced in ticks for infection of mammals. Proc. Natl. Acad. Sci. 101:3142-3147.
12. Jackson, C. A., S. D. Lovrich, W. A. Agger, and S. M. Callister. 2002. Reassessment of a midwestern Lyme disease focus for Borrelia burgdorferi and the human granulocytic ehrlichiosis agent. J. Clin. Microbiol. 40:2070-2073.
13. Jauris-Heipke, S., R. Fuchs, M. Motz, V. Preac-Mursic, E. Schwab, E. Soutschek, G. Will, and B. Wilske. 1993. Genetic heterogeneity of the genes coding for the outer surface protein C (OspC) and the flagellin of Borrelia burgdorferi. Med. Microbiol. Immunol. 182:37-50.
14. Jobe, D. A., S. D. Lovrich, R. F. Schell, and S. M. Callister. 2003. C-terminal region of outer surface protein C binds borreliacidal antibodies in sera from patients with Lyme disease. Clin. Diagn. Lab. Immunol. 10:573-578.
15. Lawrenz, M. B., J. M. Hardham, R. T. Owens, J. Nowakowski, A. C. Steere, G. P. Wormser, and S. J. Norris. 1999. Human antibody responses to VlsE antigenic variation protein of Borrelia burgdorferi. J. Clin. Microbiol. 37:3997-4004.
16. Liang, F. T., E. Aberger, M. Cinco, L. Gern, C. M. Hu, Y. N. Lobet, M. Ruscio, P. E. Voet, Jr., V. E. Weynants, and M. T. Philipp. 2000. Antigenic conservation of an immunodominant invariable region of the VlsE lipoprotein among European pathogenic genospecies of Borrelia burgdorferi SL. J. Infect. Dis. 182:1455-1462.
17. Liang, F. T., A. C. Steere, A. R. Marques, B. J. B. Johnson, J. N. Miller, and M. T. Philipp. 1999. Sensitive and specific serodiagnosis of Lyme disease by enzyme-linked immunosorbent assay with a peptide based on an immunodominant conserved region of VlsE. J. Clin. Microbiol. 37:3990-3996.
18. Lovrich, S. D., D. A. Jobe, R. F. Schell, and S. M. Callister. 2005. Borreliacidal OspC antibodies specific for a highly conserved epitope are immunodominant in human Lyme disease and do not occur in mice or hamsters. Clin. Diagn. Lab. Immunol. 12:746-751.
19. Magnarelli, L. A., J. W. Ijdo, S. J. Padula, R. A. Flavell, and E. Fikrig. 2000. Serologic diagnosis of Lyme borreliosis by using enzyme-linked immunosorbent assays with recombinant antigens. J. Clin. Microbiol. 38:1735-1739.
20. Marangoni, A., M. Sparacino, V. Mondarini, F. Cavrini, E. Storni, M. Donati, R. Cevenini, and V. Sambri. 2005. Comparative evaluation of two enzyme linked immunosorbent assay methods and three Western blot methods for the diagnosis of culture-confirmed early Lyme borreliosis in Italy. New Microbiol. 28:37-43.
21. Mathiesen, M. J., M. Christiansen, K. Hansen, A. Holm, W. Asbrink, and M. Theisen. 1998. Peptide-based OspC enzyme-linked immunosorbent assay for serodiagnosis of Lyme borreliosis. J. Clin. Microbiol. 36:3474-3479.
22. Mathiesen, M. J., A. Holm, M. Christiansen, J. Blom, E. Asbrink, and M. Thiesen. 1998. The dominant epitope of Borrelia garinii outer surface protein C recognized by sera from patients with neuroborreliosis has a surface-exposed conserved structural motif. Infect. Immun. 66:4073-4079.
23. Mogilyansky, E., C. C. Loa, M. E. Adelson, E. Mordechai, and R. C. Tilton. 2004. Comparison of Western immunoblotting and the C6 Lyme antibody test for laboratory detection of Lyme disease. Clin. Diagn. Lab. Immunol. 11:924-929.
24. Padilla, M. L., S. M. Callister, R. F. Schell, G. L. Bryant, D. A. Jobe, S. D. Lovrich, B. K. DuChateau, and J. R. Jensen. 1996. Characterization of the protective borreliacidal antibody response in humans and hamsters after vaccination with a Borrelia burgdorferi outer surface protein A vaccine. J. Infect. Dis. 174:739-746.
25. Padula, S. J., F. Dias, A. Sampieri, R. B. Craven, and R. W. Ryan. 1994. Use of recombinant OspC from Borrelia burgdorferi for serodiagnosis of early Lyme disease. J. Clin. Microbiol. 32:1733-1738.
26. Petrakis, N. L. 1985. Biologic banking in cohort studies, with special reference to blood. NCI Monogr. 67:193-198.
27. Rousselle, J. C., S. M. Callister, R. F. Schell, S. D. Lovrich, D. A. Jobe, J. A. Marks, and C. A. Wieneke. 1998. Borreliacidal antibody production against outer surface protein C of Borrelia burgdorferi. J. Infect. Dis. 178:733-741.
28. Schwan, T. G., J. Piesman, W. T. Golde, M. C. Dolan, and P. A. Rosa. 1995. Induction of an outer surface protein on Borrelia burgdorferi during tick feeding. Proc. Natl. Acad. Sci. USA 92:2909-2913.
29. Smismans, A., V. J. Goossens, E. Nulens, and C. A. Bruggeman. 2006. Comparison of five different immunoassays for the detection of Borrelia burgdorferi IgM and IgG antibodies. Clin. Microbiol. Infect. 12:648-655.
30. Steere, A. C., V. K. Sikand, F. Meurice, D. L. Parenti, E. Fikrig, R. T. Schoen, J. Nowakowski, C. H. Schmid, S. Laukamp, C. Buscarino, and D. S. Krause. 1998. Vaccination against Lyme disease with recombinant Borrelia burgdorferi outer-surface lipoprotein A with adjuvant. N. Engl. J. Med. 339:209-215.
31. Theisen, M., B. Frederiksen, A. M. Lebech, J. Vuust, and K. Hansen. 1993. Polymorphism in ospC gene of Borrelia burgdorferi and immunoreactivity of OspC protein: implications for taxonomy and for use of OspC protein as a diagnostic antigen. J. Clin. Microbiol. 31:2570-2576.
32. Tilly, K., J. G. Krum, A. Bestor, M. W. Jewett, D. Grimm, D. Bueschel, R. Byram, D. Dorward, P. Stewart, and P. A. Rosa. 2006. Borrelia burgdorferi OspC protein required exclusively in a crucial early stage of mammalian infection. Infect. Immun. 74:3554-3564.
33. Wieneke, C. A., S. D. Lovrich, S. M. Callister, D. A. Jobe, J. A. Marks, and R. F. Schell. 2000. Evaluation of whole-cell and OspC enzyme-linked immunosorbent assays for discrimination of early Lyme borreliosis from OspA vaccination. J. Clin. Microbiol. 38:313-317.