Activity of abrB310 promoter in wild type and spo0A-deficient strains of Clostridium acetobutylicum

Journal of Industrial Microbiology and Biotechnology

Volumn 35, Issue 7, 2008, Pages 743-750

  Scotcher, Miles C ^a   Bennett, George N ^a  

^a RICE UNIVERSITY   (United States)

Author keywords

Clostridium; Metabolite; Promoter; Solvent; Sporulation

Indexed keywords

BETA GALACTOSIDASE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BINDING SITE; CLOSTRIDIUM ACETOBUTYLICUM; CONTROLLED STUDY; EXPRESSION VECTOR; GENE ACTIVITY; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; NONHUMAN; PROMOTER REGION; REPORTER GENE; TRANSCRIPTION INITIATION SITE; WILD TYPE;

ARTIFICIAL GENE FUSION; BASE SEQUENCE; BETA-GALACTOSIDASE; BINDING SITES; CLOSTRIDIUM ACETOBUTYLICUM; GENE DELETION; GENE EXPRESSION; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); RNA, BACTERIAL; RNA, MESSENGER; SEQUENCE DELETION; TRANSCRIPTION FACTORS; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION, GENETIC;

CLOSTRIDIUM; CLOSTRIDIUM ACETOBUTYLICUM;

EID: 46949101196     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-008-0341-x     Document Type: Article

References (22)

1. Mitchell WJ (1998) Physiology of carbohydrate to solvent conversion by clostridia. Adv Microb Physiol 39:31-130
2. Harris LM (2001) Cloning and characterization of the Clostridium acetobutylicum ATCC824 gene encoding the Spo0A transcription regulator and its role in controlling solvent formation and sporulation-specific gene expression. Ph.D. dissertation. Northwestern University, Evanston
3. Harris LM, Welker NE, Papoutsakis ET (2002) Northern, morphological, and fermentation analysis of spo0A inactivation and overexpression in Clostridium acetobutylicum ATCC 824. J Bacteriol 184:3586-3597
4. Tomas CA, Alsaker KV, Bonarius HP, Hendriksen WT, Yang H, Beamish JA, Paredes CJ, Papoutsakis ET (2003) DNA array-based transcriptional analysis of asporogenous, nonsolventogenic Clostridium acetobutylicum strains SKO1 and M5. J Bacteriol 185:4539-4547
5. Alsaker KV, Papoutasakis ET (2005) Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum. J Bacteriol 187:7103-7118
6. Sullivan L, Bennett GN (2006) Proteome analysis and comparison of Clostridium acetobutylicum ATCC 824 and Spo0A strain variants. J Ind Microbiol Biotechnol 33:298-308
7. Scotcher MC, Bennett GN (2005) SpoIIE regulates sporulation but does not directly affect solventogenesis in Clostridium acetobutylicum ATCC 824. J Bacteriol 187:1930-1936
8. Scotcher MC, Rudolph FB, Bennett GN (2005) Expression of abrB310 and SinR, and effects of decreased abrB310 expression on the transition from acidogenesis to solventogenesis, in Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 71:1987-1995
9. Strauch MA, Perego M, Burbulys D, Hoch JA (1989) The transition state transcription regulator AbrB of Bacillus subtilis is autoregulated during vegetative growth. Mol Microbiol 3:1203-1209
10. Strauch MA, Spiegelman GB, Perego M, Johnson WC, Burbulys D, Hoch JA (1989) The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein. EMBO J 8:1615-1621
11. Hahn J, Roggiani M, Dubnau D (1995) The major role of Spo0A in genetic competence is to downregulate abrB, an essential competence gene. J Bacteriol 177:3601-3605
12. Greene EA, Spiegelman GB (1996) The Spo0A protein of Bacillus subtilis inhibits transcription of the abrB gene without preventing binding of the polymerase to the promoter. J Biol Chem 271:11455-11461
13. Strauch M, Webb V, Spiegelman G, Hoch JA (1990) The Spo0A protein of Bacillus subtilis is a repressor of the abrB gene. Proc Natl Acad Sci USA 87:1801-1805
14. Stragier P, Losick R (1996) Molecular genetics of sporulation in Bacillus subtilis. Annu Rev Genet 30:297-341
15. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
16. Hartmanis MGN, Gatenbeck S (1984) Intermediary metabolism in Clostridium acetobutylicum: levels of enzymes involved in the formation of acetate and butyrate. Appl Environ Microbiol 47:1277-1283
17. Zhao Y, Hindorff LA, Chuang A, Monroe-Augustus M, Lyristis M, Harrison ML, Rudolph FB, Bennett GN (2003) Expression of a cloned cyclopropane fatty acid synthase gene reduces solvent formation in Clostridium acetobutylicum ATCC824. Appl Environ Microbiol 69:2831-2841
18. Mermelstein LD, Welker NE, Bennett GN, Papoutsakis ET (1992) Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824. Biotechnology 10:190-195
19. Green EM, Boynton ZL, Harris LM, Rudolph FB, Papoutsakis ET, Bennett GN (1996) Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824. Microbiology 142:2079-2086
20. Burchhardt G, Bahl H (1991) Cloning and analysis of the beta-galactosidase-encoding gene from Clostridium thermosulfurogenes EM1. Gene 106:13-19
21. Tummala SB, Welker NE, Papoutsakis ET (1999) Development and characterization of a gene expression reporter system for Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 65:3793-3799
22. Shafikhani SH, Leighton T (2004) AbrB and Spo0E control the proper timing of sporulation in Bacillus subtilis. Curr Microbiol 48:262-269