Role of conserved aspartates in the ArsA ATPase

Biochemistry

Volumn 47, Issue 27, 2008, Pages 7218-7227

  Bhattacharjee, Hiranmoy ^a   Choudhury, Ranginee ^a,b   Rosen, Barry P ^a  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINETRIPHOSPHATE; ESCHERICHIA COLI; HEALTH; METALS; NUCLEOTIDES;

ACTIVATION DOMAINS; ASPARTATES; ATP-ASE ACTIVITY; BINDING DOMAIN (BD); CATALYTIC SITES; CATALYTIC SUBUNITS; INFORMATION COMMUNICATIONS; NUCLEOTIDE-BINDING DOMAIN (NBD1);

NUCLEIC ACIDS;

ADENOSINE TRIPHOSPHATASE; ALANINE; ANTIMONY DERIVATIVE; ARSA ADENOSINE TRIPHOSPHATASE; ARSENIC DERIVATIVE; ASPARAGINE; ASPARTIC ACID; GLUTAMIC ACID; MAGNESIUM ION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME LOCALIZATION; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; ARSENITES; ASPARTIC ACID; CATALYSIS; CONSERVED SEQUENCE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLUORESCENCE; ION PUMPS; KINETICS; MAGNESIUM; MULTIENZYME COMPLEXES; MUTANT PROTEINS; PROTEIN STRUCTURE, TERTIARY; STRUCTURE-ACTIVITY RELATIONSHIP; TRYPSIN; TRYPTOPHAN;

ESCHERICHIA COLI;

EID: 46849108282     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi800715h     Document Type: Article

References (30)

1. Abernathy, C. O., Thomas, D. J., and Calderon, R. L. (2003) Health effects and risk assessment of arsenic. J. Nutr. 133, 1536S-1538S.
2. Bhattacharjee, H., and Rosen, B. P. (2007) Arsenic Metabolism in Prokaryotic and Eukaryotic Microbes, in Molecular Microbiology of Heavy Metals (Nies, D. H. , and Silver, S., Eds.) pp 371-406, Springer, Berlin/Heidelberg.
3. Tisa, L. S., and Rosen, B. P. (1990) Molecular characterization of an anion pump. The ArsB protein is the membrane anchor for the ArsA protein. J. Biol. Chem. 265, 190-194.
4. Rosen, B. P., Weigel, U., Karkaria, C., and Gangola, P. (1988) Molecular characterization of an anion pump. The arsA gene product is an arsenite (antimonate)-stimulated ATPase. J. Biol. Chem. 263, 3067-3070.
5. Li, J., and Rosen, B. P. (2000) The linker peptide of the ArsA ATPase. Mol. Microbiol. 35, 361-367.
6. Karkaria, C. E., Chen, C. M., and Rosen, B. P. (1990) Mutagenesis of a nucleotide-binding site of an anion-translocating ATPase. J. Biol. Chem. 265, 7832-7836.
7. Kaur, P., and Rosen, B. P. (1992) Mutagenesis of the C-terminal nucleotide-binding site of an anion-translocating ATPase. J. Biol. Chem. 267, 19272-19277.
8. Zhou, T., Radaev, S., Rosen, B. P., and Gatti, D. L. (2000) Structure of the ArsA ATPase: the catalytic subunit of a heavy metal resistance pump. EMBO J. 19, 4838-4845.
9. Ruan, X., Bhattacharjee, H., and Rosen, B. P. (2008) Characterization of the metalloactivation domain of an arsenite/antimonite resistance pump. Mol. Microbiol. 67, 392-402.
10. Zhou, T., Radaev, S., Rosen, B. P., and Gatti, D. L. (2001) Conformational changes in four regions of the Escherichia coli ArsA ATPase link ATP hydrolysis to ion translocation. J. Biol. Chem. 276, 30414-30422.
11. Jiang, Y., Bhattacharjee, H., Zhou, T., Rosen, B. P., Ambudkar, S. V., and Sauna, Z. E. (2005) Nonequivalence of the nucleotide binding domains of the ArsA ATPase. J. Biol. Chem. 280, 9921-9926.
12. 2+ ligand in the ArsA ATPase. J. Biol. Chem. 274, 13854-13858.
13. Bhattacharjee, H., and Rosen, B. P. (2000) Role of conserved histidine residues in metalloactivation of the ArsA ATPase. Biometals 13, 281-288.
14. Sambrook, J., and Russell, D. (2001) Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. Zhou, T., and Rosen, B. P. (1997) Tryptophan fluorescence reports nucleotide-induced conformational changes in a domain of the ArsA ATPase. J. Biol. Chem. 272, 19731-19737.
16. Chung, C. T., Niemela, S. L., and Miller, R. H. (1989) One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. U.S.A. 86, 2172-2175.
17. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
18. Bhattacharjee, H., and Rosen, B. P. (2001) Structure-function analysis of the ArsA ATPase: contribution of histidine residues. J. Bioenerg. Biomembr. 33, 459-468.
19. Kuroda, M., Bhattacharjee, H., and Rosen, B. P. (1998) Arsenical pumps in prokaryotes and eukaryotes. Methods Enzymol. 292, 82-97.
20. Hsu, C. M., and Rosen, B. P. (1989) Characterization of the catalytic subunit of an anion pump. J. Biol. Chem. 264, 17349-17354.
21. Zhou, T., Liu, S., and Rosen, B. P. (1995) Interaction of substrate and effector binding sites in the ArsA ATPase. Biochemistry 34, 13622-13626.
22. Walmsley, A. R., Zhou, T., Borges-Walmsley, M. I., and Rosen, B. P. (1999) The ATPase mechanism of ArsA, the catalytic subunit of the arsenite pump. J. Biol. Chem. 274, 16153-16161.
23. Walmsley, A. R., Zhou, T., Borges-Walmsley, M. I., and Rosen, B. P. (2001) Antimonite regulation of the ATPase activity of ArsA, the catalytic subunit of the arsenical pump. Biochem. J. 360, 589-597.
24. Walmsley, A. R., Zhou, T., Borges-Walmsley, M. I., and Rosen, B. P. (2001) A kinetic model for the action of a resistance efflux pump. J. Biol. Chem. 276, 6378-6391.
25. Kaur, P. (1999) The anion-stimulated ATPase ArsA shows unisite and multisite catalytic activity. J. Biol. Chem. 274, 25849-25854.
26. Ruan, X., Bhattacharjee, H., and Rosen, B. P. (2006) Cys-113 and Cys-422 form a high affinity metalloid binding site in the ArsA ATPase. J. Biol. Chem. 281, 9925-9934.
27. Ramaswamy, S., and Kaur, P. (1998) Nucleotide binding to the C-terminal nucleotide binding domain of ArsA: Studies with an ATP analogue, 5′-p-fluorosulfonylbenzoyladenosine. J. Biol. Chem. 273, 9243-9248.
28. Rosen, B. P., Bhattacharjee, H., Zhou, T., and Walmsley, A. R. (1999) Mechanism of the ArsA ATPase. Biochim. Biophys. Acta 1461, 207-215.
29. Lusk, J. E., Williams, R. J., and Kennedy, E. P. (1968) Magnesium and the growth of Escherichia coli. J. Biol. Chem. 243, 2618-2624.
30. Li, J., Liu, S., and Rosen, B. P. (1996) Interaction of ATP binding sites in the ArsA ATPase, the catalytic subunit of the Ars pump. J. Biol. Chem. 271, 25247-25252.