Identification of Leishmania parasites in clinical samples obtained from cutaneous leishmaniasis patients using PCR-RFLP technique in endemic region, Sanliurfa province, in Turkey

Parasitology Research

Volumn 103, Issue 3, 2008, Pages 583-586

  Akkafa, Feridun ^a   Dilmec, Fuat ^a   Alpua, Zuhre ^b  

^a HARRAN UNIVERSITY   (Turkey)

^b City Directorate of Health   (Turkey)

Author keywords

[No Author keywords available]

Indexed keywords

RESTRICTION ENDONUCLEASE;

ADOLESCENT; ADULT; ARTICLE; CHILD; DNA ISOLATION; ENDEMIC DISEASE; EXUDATE; FEMALE; GENE AMPLIFICATION; HUMAN; INTERMETHOD COMPARISON; LEISHMANIA; LEISHMANIA TROPICA; MALE; MICROSCOPY; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PREVALENCE; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RESTRICTION MAPPING; SENSITIVITY AND SPECIFICITY; SKIN LEISHMANIASIS; TURKEY (REPUBLIC);

ADOLESCENT; ADULT; ANIMALS; CHILD, PRESCHOOL; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA PRIMERS; DNA, PROTOZOAN; ENDEMIC DISEASES; FEMALE; HUMANS; INFANT; LEISHMANIA; LEISHMANIASIS, CUTANEOUS; MALE; MICROSCOPY; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SENSITIVITY AND SPECIFICITY; TURKEY;

LEISHMANIA TROPICA;

EID: 46749156852     PISSN: 09320113     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00436-008-1013-5     Document Type: Article

References (14)

1. Belli A, Rodriguez B, Aviles H, Harris E (1998) Simplified polymerase chain reaction detection of new world leishmania in clinical specimens of cutaneous leishmaniasis. Am J Trop Med Hyg 58:102-109
2. Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL (2006) Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol 44:1435-1439
3. Chargui N, Bastien P, Kallel K, Haouas N, Akrout FM, Masmoudi A, Zili J, Chaker E, Othman AD, Azaiez R, Crobu L, Mezhoud H, Babba H (2005) Usefulness of PCR in the diagnosis of cutaneous leishmaniasis in Tunisia. Trans R Soc Trop Med Hyg 99:762-768
4. Culha G, Uzun S, Ozcan K, Memisoglu HR, Chang KP (2006) Comparison of conventional and polymerase chain reaction diagnostic techniques for leishmaniasis in the endemic region of Adana, Turkey. Int J Dermatol 45:569-572
5. de Oliveira CI, Bafica A, Oliveira F, Favali CB, Correa T, Freitas LA, Nascimento E, Costa JM, Barral A (2003) Clinical utility of polymerase chain reaction-based detection of leishmania in the diagnosis of American cutaneous leishmaniasis. Clin Infect Dis 37:149-153
6. Knio KN, Baydoun E, Tawk R, Nuwayri-Salti N (2000) Isoenzyme characterization of leishmania isolates from Lebanon and Syria. Am J Trop Med Hyg 63:43-47
7. Laskay T, Miko TL, Negesse Y, Solbach W, Röllinghoff M, Frommel D (1995) Detection of cutaneous leishmania infection in paraffin-embedded skin biopsies using the polymerase chain reaction. Trans R Soc Trop Med Hyg 89:273-275
8. Marfurt J, Nasereddin A, Niederwieser I, Jaffe CL, Beck HP, Felger I (2003) Identification and differentiation of leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis. J Clin Microbiol 41:3147-3153
9. Minodier P, Piarroux R, Gambarelli F, Joblet C, Dumon H (1997) Rapid identification of causative species in patients with Old World leishmaniasis. J Clin Microbiol 35:2551-2555
10. Ok UZ, Balcioglu IC, Taylan Ozkan A, Ozensoy S, Ozbel Y (2002) Leishmaniasis in Turkey. Acta Trop 84:43-48
11. Talari SA, Talaei R, Shajari G, Vakili Z, Taghaviardakani A (2006) Childhood cutaneous leishmaniasis: report of 117 cases from Iran. Korean J Parasitol 44:355-360
12. Uzun S, Uslular C, Yucel A, Acar MA, Ozpoyraz M, Memisoglu H (1999) Cutaneous leishmaniasis: evaluation of 3,074 cases in the Cukurova region of Turkey. Br J Dermatol 140:347-350
13. Vega-Lopez F (2003) Diagnosis of cutaneous leishmaniasis. Curr Opin Infect Dis 16:97-101
14. Weigle KA, de Davalos M, Heredia P, Molineros R, Saravia NG, D'Alessandro A (1987) Diagnosis of cutaneous and mucocutaneous leishmaniasis in Colombia: a comparison of seven methods. Am J Trop Med Hyg 36:489-496