Spirodela (duckweed) as an alternative production system for pharmaceuticals: A case study, aprotinin

Transgenic Research

Volumn 17, Issue 4, 2008, Pages 503-513

  Rival, Sandrine ^a   Wisniewski, Jean Pierre ^a   Langlais, Audrey ^a   Kaplan, Hélène ^a   Freyssinet, Georges ^a,b   Vancanneyt, Guy ^c   Vunsh, Ron ^d   Perl, Avihai ^d,e   Edelman, Marvin ^d  

^a LemnaGene SA   (France)

^b Limagrain ^*  (France)

^c Bayer sa nv Belgium   (Belgium)

^d WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^e VOLCANI CENTER   (Israel)

Author keywords

Aprotinin; Lemnaceae; Pharmaceutical protein; Spirodela oligorrhiza; Transgenic plants

Indexed keywords

APROTININ; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; TRYPSIN INHIBITOR;

ARACEAE; ARTICLE; GENE EXPRESSION REGULATION; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; NORTHERN BLOTTING; PHYSIOLOGY; PLASMID; PROMOTER REGION; SOUTHERN BLOTTING; TRANSGENE; TRANSGENIC PLANT; WESTERN BLOTTING;

APROTININ; ARACEAE; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; BLOTTING, WESTERN; GENE EXPRESSION REGULATION, PLANT; PLANTS, GENETICALLY MODIFIED; PLASMIDS; PROMOTER REGIONS (GENETICS); PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC; TRANSGENES; TRYPSIN INHIBITORS;

AGROBACTERIUM; ARACEAE; LEMNA; RHIZOBIUM; SPIRODELA; SPIRODELA PUNCTATA;

EID: 46649092540     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-007-9123-x     Document Type: Article

References (23)

1. Apeler H, Peters J, Schröder W, Schneider KH, Lemm G, Hinz V, Rossouw GJ, Dembowsky K (2004) Expression, purification, biochemical and pharmacological characterization of a recombinant aprotinin variant. Biotechnol Drug Res 8:483-497
2. Cox KM, Sterling JD, Regan JT, Gasdaska JR, Frantz KK, Peele CG, Black A, Passmore D, Moldovan-Loomis C, Srinivasan M, Cuison S, Cardarelli PM, Dickey LF (2006) Glycan optimization of a human monoclonal antibody in the aquatic plant Lemna minor. Nature Biotechnol 24:1591-1597
3. Davies MJ, Allen A, Kort H, Weerasena NA, Rocco D, Paul CL, Hunt BJ, Elliot MJ (1997) Prospective, randomized, double-blind study of high-dose aprotinin in paediatric cardiac operations. Ann Thoracic Surg 63:497-503
4. Delaney D, Jilka J, Barker D, Irwin B, Poage M, Woodard S, Horn M, Vinas A, Beifus K, Marker M, Wiggins B, Drees C, Harkey R, Nikolov Z, Hood E, Howard J, (2003) Production of aprotinin in transgenic maize seeds for the pharmaceutical and cell culture markets. In: Vasil IK (ed) Plant biotechnology 2002 and beyond. Proceedings of the 10th IAPTC&B Congress, Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 393-394
5. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1(4):19-21
6. Edelman M, Perl A, Flaishman M, Blumenthal A (1998) Transgenic Lemnaceae. International application published under the Patent Cooperation Treaty (PCT) WO 99/19497, pp 1-56
7. Edelman M, Vunsh R, LI J, Hanania U, Flaishman M, Perl A (2003) Transgenic Spirodela: a unique, low-risk, plant biotechnology system. In plant biology 2003 program, Biotech risk assesment. Abstract 955, p 196. Ed. American Society of Plant Physiology
8. Fischer R, Stoger E, Schillberg S, Christou P, Twyman RM (2004) Plant-based production of biopharmaceuticals. Current Opin Plant Biol 7:152-158
9. Freyssinet G, Langlais A, Rival S, Wisniewsky JP (2005) Production of vaccines and drugs in Lemnaceae. Conference on plant-made pharmaceuticals, pp 75
10. Gasdaska JR, Spencer D, Dickey L (2003) Advantages of therapeutic protein production in aquatic plant Lemna. BioProcessing J 3:50-56
11. Hood EE (2000) New century, new technology, new products. Transgenic Res 9:277-299
12. Jansen MAK, Hill LM, Thorneley RNF (2004) A novel stress-acclimation response in Spirodela punctata (Lemnaceae): 2,4,6-trichlorophenol triggers an increase in the level of an extracellular peroxidase, capable of the oxidative dechlorination of this xenobiotic pollutant. Plant Cell Environ 27:603-613
13. Kyhse-Anderson J (1984) Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biophys Biochem Method 10:203-209
14. Laskowski M, Kato I (1980) Protein inhibitor of proteinases. Ann Rev Biochem 49:593-626
15. Li J, Jain M, Vunsh R, Vishnevetsky J, Hanania U, Flaishman M, Perl A, Edelman M, (2004) Callus induction and regeneration in Spirodela and Lemna. Plant Cell Rep 22:457-464
16. Ma JKC, Chikwamba R, Sparrow P, Fischer R, Mahoney R, Twyman RM (2005) Plant-derived pharmaceuticals - the road forward. Trends Plant Sci 10:580-585
17. Molle V, Zanella-Cleon I, Robin JP, Mallejac S, Cozzone AJ, Becchi M (2006) Characterization of the phosphorylation sites of Mycobacterium tuberculosis serine/threonine protein kinases, PknA, PknD, PknE, and PknH by mass spectrometry
18. Shenk RU, Hildebrandt AC (1972) Medium and technique for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199-204
19. Stomp AM (2005) The Duckweeds - a valuable plant for biomanufacturing. Biotechnol Ann Rev 11:69-99
20. Van Engelen FA, Molthoff JW, Conner AJ, Nap JP, Pereira A, Stiekema WJ (1995) pBINPLUS: an improved plant transformation vector based on pBIN19. Transgenic Res 4:288-290
21. Wu G, Bashir-Bello N, Freeland SJ (2006) The synthetic gene designer: a flexible web platform to explore sequence manipulation for heterologous expression. Protein Expr Purif 47:441-445
22. Xu H, Montoya FU, Wang Z, Lee JM, Reeves R, Linthicum DS, Magnuson NS (2002) Combined used of regulatory elements within the cDNA to increase the production of a soluble mouse single-chain antibody, scFv, from tobacco cell suspension cultures. Protein Expr Purif 24:384-394
23. Zhong GY, Peterson D, Delaney DE, Bailey M, Witcher DR, Register JC III, Bond D, Li CP, Marshall L, Kulisek E, Ritland D, Meyer T, Hood EE, Howard JA (1999) Commercial production of aprotinin in transgenic maize seeds. Mol Breed 5:345-356