Assessment of protoxin composition of Bacillus thuringiensis strains by use of polyacrylamide gel block and mass spectrometry

Applied Microbiology and Biotechnology

Volumn 79, Issue 5, 2008, Pages 875-880

  Fu, Zujiao ^a   Sun, Yunjun ^a   Xia, Liqiu ^a   Ding, Xuezhi ^a   Mo, Xiangtao ^a   Li, Xiaohui ^a   Huang, Kexue ^a   Zhang, Youming ^a  

^a HUNAN NORMAL UNIVERSITY   (China)

Author keywords

Bacillus thuringiensis; Insecticidal activity; LC MS MS; Polyacrylamide gel block; Protoxin expression profile

Indexed keywords

BLOCK COPOLYMERS; GENE EXPRESSION; INSECTICIDES; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; POLYACRYLATES; SOLUBILITY;

BACILLUS THURINGIENSIS; INSECTICIDAL ACTIVITY; POLYACRYLAMIDE GEL BLOCK; PROTOXIN EXPRESSION PROFILE;

BACTERIA;

POLYACRYLAMIDE GEL; PROTOXIN;

ASSESSMENT METHOD; BACTERIUM; CRYSTAL; HOMOLOGY; INSECTICIDE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; TOXIN;

ANALYTIC METHOD; ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL STRAIN; EXPERIMENTAL MODEL; INSECTICIDAL ACTIVITY; LIQUID CHROMATOGRAPHY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; SCREENING TEST; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY; SOLUBILIZATION; SUBSPECIES; TANDEM MASS SPECTROMETRY; TOXIN ANALYSIS;

ACRYLIC RESINS; BACILLUS THURINGIENSIS; BACTERIAL TOXINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MASS SPECTROMETRY; MOLECULAR WEIGHT; PROTEIN PRECURSORS;

BACILLUS THURINGIENSIS;

EID: 46549085508     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1488-0     Document Type: Article

References (26)

1. Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI (2002) Directed proteomic analysis of the human nucleolus. Curr Biol 12:1-11
2. Ben-Dov E, Boussiba S, Zaritsky A (1995) Mosquito larvicidal activity of Escherichia coli with combinations of genes from Bacillus thuringiensis subsp. israelensis. J Bacteriol 177:2851-2857
3. Berry C, O'Neil S, Ben-Dov E, Jones AF, Murphy L, Quail MA, Holden MT, Harris D, Zaritsky A, Parkhill J (2002) Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol 68:5082-5095
4. Delécluse A, Charles JF, Klier A, Rapoport G (1991) Deletion by in vivo recombination shows that the 28-kilodalton cytolytic polypeptide from Bacillus thuringiensis subsp. israelensis is not essential for mosquitocidal activity. J Bacteriol 173:3374-3381
5. Garduno F, Thorne L, Walfield AM, Pollock TJ (1988) Structural relatedness between mosquitocidal endotoxins of Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol 54:277-279
6. Güereca L, Bravo A (1999) The oligomeric state of Bacillus thuringiensis Cry toxins in solution. Biochim Biophys Acta 1429:342-350
7. Han DK, Eng J, Zhou H, Aebersold R (2001) Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry. Nat Biotechnol 19:946-951
8. Henzel WJ, Billeci TM, Stults JT, Wong SC, Grimley C, Watanabe C (1993) Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases. Proc Natl Acad Sci USA 90:5011-5015
9. Höfte H, Whiteley HR (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev 53:242-255
10. Hu X, Hansen BM, Yuan Z, Johansen JE, Eilenberg J, Hendriksen NB, Smidt L, Jensen GB (2005) Transfer and expression of the mosquitocidal plasmid pBtoxis in Bacillus cereus group strains. FEMS Microbiol Lett 245:239-247
11. Kolstø AB, Grønstad A, Oppegaard H (1990) Physical map of the Bacillus cereus chromosome. J Bacteriol 172:3821-3525
12. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
13. Lee KY, Kang EY, Park S, Ahn SK, Yoo KH, Kim JY, Lee HH (2006) Mass spectrometric sequencing of endotoxin proteins of Bacillus thuringiensis ssp. konkukian extracted from polyacrylamide gels. Proteomics 6:1512-1517
14. Masson L, Erlandson M, Puzstai-Carey M, Brousseau R, Juarez-Perez V, Frutos R (1998) A holistic approach for determining the entomopathogenic potential of Bacillus thuringiensis strains. Appl. Environ. Microbiol 64:4782-4788
15. McCarthy FM, Burgess SC, van den Berg BH, Koter MD, Pharr GT (2005) Differential detergent fractionation for non-electrophoretic eukaryote cell proteomics. J Proteome Res 4:316-324
16. Porcar M, Caballero P (2000) Molecular and insecticidal characterization of a Bacillus thuringiensis strain isolated during a natural epizootic. J Appl Microbiol 89:309-316
17. Rabilloud T (2002) Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains. Proteomics 2:3-10
18. Ranasinghe C, Akhurst RJ (2002) Matrix assisted laser desorption ionisation time of flight mass spectrometry (MALDI-TOF MS) for detecting novel Bt toxins. J Invertebr Pathol 79:51-58
19. Rappsilber J, Ryder U, Lamond AI, Mann M (2002) Large-scale proteomic analysis of the human spliceosome. Genome Res 12:1231-1245
20. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, third edition. Cold Spring Harbor, Cold Spring Harbor, New York
21. Schirle M, Heurtier MA, Kuster B (2003) Profiling core proteomes of human cell lines by one-dimensional PAGE and liquid chromatography-tandem mass spectrometry. Mol Cell Proteomics 2:1297-1305
22. Schnepf E, Crickmore N, Van Rie J, Lereclus D, Baum J, Feitelson J, Zeigler DR, Dean DH (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev 62:775-806
23. van Frankenhuyzen K, Milne R, Brousseau R, Masson L (1992) Comparative toxicity of the HD-1 and NRD-12 strains of Bacillus thuringiensis subsp. kurstaki to defoliating forest Lepidoptera. J Invertebr Pathol 59:149-154
24. Washburn MP, Yates JR III (2000) Analysis of the microbial proteome. Curr Opin Microbiol 3:292-297
25. Washburn MP, Wolters D, Yates JR III (2001) Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat Biotechnol 19:242-247
26. Zhou J, Zhou T, Cao R, Liu Z, Shen J, Chen P, Wang X, Liang S (2006) Evaluation of the application of sodium deoxycholate to proteomic analysis of rat hippocampal plasma membrane. J Proteome Res 5:2547-2553