Flow cytometry in plant breeding

Cytometry Part A

Volumn 73, Issue 7, 2008, Pages 581-598

  Ochatt, Sergio J ^a  

^a CEMAGREF   (France)

Author keywords

C DNA content; Cell wall fractions; Chromosome sorting; Physical mapping of genomes; Plant cell cytogenetics; Ploidy level determination; Regeneration competence; Somatic hybrids; True to typeness

Indexed keywords

CELL NUCLEUS DNA;

ACCURACY; DNA CONTENT; FLOW CYTOMETRY; NONHUMAN; PLANT BREEDING; PLANT CELL; PLANT DEVELOPMENT; PLANT GROWTH; PLOIDY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVIEW;

BOTANY; CELL NUCLEUS; CROSSES, GENETIC; DNA, PLANT; EQUIPMENT DESIGN; FABACEAE; FLOW CYTOMETRY; GENOTYPE; IMMUNOPHENOTYPING; OPTICS; PLANT PHYSIOLOGY; PLANTS; PLOIDIES;

EID: 46449129363     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20562     Document Type: Review

References (151)

1. Shapiro HM. Practical Flow Cytometry, 4th ed. USA: Wiley-Liss; 2003. pp 736. ISBN: 978-0-471-41125-3.
2. Shapiro HM. Cytometry and cytometers: Development and growth. In: Doležel J, Greilhuber J, Suda J, editors. Flow Cytometry with Plant Cells. Analysis of Genes, Chromosomes and Genomes. Weinheim: Wiley-VCH; 2007. pp 1-17. KgaA, ISBN: 978-3-527-31487-4.
3. Galbraith DW, Bartoš J, Doležel J. Flow cytometry and cell sorting in plant biotechnology. In: Sklar LA, editor. Flow Cytometry for Biotechnology. Oxford: Oxford University Press; 2005. pp 291-322.
4. Doležel J, Greilhuber J, Suda J, editors. Flow Cytometry with Plant Cells. Analysis of Genes, Chromosomes and Genomes. Weinheim: Wiley-VCH; 2007. 454 pp. KgaA, ISBN: 978-3-527-31487-4.
5. Ochatt SJ. How cytometry: Ploidy determination, cell cycle analysis, DNA content per nucleus. In: Medicago truncatula Handbook, version November 2006,13 p. Available at http://www.noble.org/medicagohandbook/index.html. ISBN 0-9754303-1-9.
6. Doležel J, Greilhuber J, Suda J. Flow cytometry with plants: An overview. In: Doležel J, Greilhuber J, Suda J, editors. Flow Cytometry with Plant Cells. Analysis of Genes, Chromosomes and Genomes. Weinheim: Wiley-VCH; 2007. pp 41-65. KgaA, ISBN: 978-3-527-31487-4
7. Robinson JP. Introduction to flow cytometry. Flow cytometry talks. USA: Purdue University Cytometry Laboratory; 2006. Available at: http://www.cyto. purdue.edu/flowcyt/educate/pptslide.htm
8. Murphy RF. Basic flow cytometry theory. Flow cytometry talks. USA: Purdue University Cytometry Laboratory; 2006. Available at: http://www.cyto.purdue. edu/flowcyt/educate/pptslide.htm
9. Heller FO. DNA measurement of Vicia faba L. with the pulse cytophotometry. Ber Deutsch Bot Gesellsch Bd 1973;86:437-441.
10. Shapiro HM, Perlmutter NG. Violet laser diodes as light sources for cytometry. Cytometry 2001;44:133-136.
11. Galbraith DW, Harkins KR, Maddox JR, Ayres NM, Sharma DP, Firrozabady E. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 1983;220:1049-1051.
12. Loureiro J, Rodriguez E, Doležel J, Santos G. Comparison of four nuclear isolation buffers for plant DNA flow cytometry. Ann Bot 2006;98:679-689.
13. Cocking EC. A method for the isolation of plant protoplasts and vacuoles. Nature 1960;187:927-929.
14. Ochatt SJ, Power JB. Plant regeneration from cultured protoplasts of higher plants. In: Moo-Young M, Warren GS, Fowler MW, editors. Comprehensive Biotechnology, Second Supplement. New York: Pergamon; 1992. pp 99-127.
15. Harkins KR, Galbraith DW. Flow sorting and culture of plant protoplasts. Physiol Plantarum 1984;60:43-52.
16. Galbraith DW, Afonso CL, Harkins KR. Flow sorting and culture of protoplasts: Conditions for high frequency recovery and growth of sorted protoplasts of suspension cultures of Nicotiana. Plant Cell Rep 1984;3:151-155.
17. Ochatt SJ, Marget P, Aubert G, Moussy F, Pontécaille G, Jacas L. Overcoming hybridisation barriers between pea and some of its wild relatives. Euphytica 2004;137:353-359.
18. Koné M, Patat-Ochatt EM, Conreux C, Sangwan RS, Ochatt SJ. In vitro morphogenesis from cotyledon and epicotyl explants and flow cytometry distinction between landraces of Bambara groundnut [Vigna subterranea (L.) Verde] an underutilised grain legume. Plant Cell Tissue Organ Cult 2007;88:61-75.
19. Bennett MD, Smith J. Nuclear DNA amounts in angiosperms. Phil Trans R Soc B 1976;274:227-274.
20. Bennett MD, Leitch IJ. Nuclear DNA amounts in angiosperms - Five eighty three new estimates. Ann Bot 1997;80:169-196.
21. Bennett MD, Leitch IJ. Nuclear DNA amounts in angiosperms: Progress, problems and prospects. Ann Bot 2005;95:45-90.
22. Zonneveld BJM, Leitch IJ, Bennett MD. First nuclear DNA amounts in more than 300 angiosperms. Ann But 2005;96:229-244.
23. Loureiro J, Rodriguez E, Doležel J, Santos C. Flow cytometric and microscopic analysis of the effect of tannic acid on plant nuclei and estimation of DNA content. Ann Bot 2006;98:515-527.
24. Cvikrová M, Binarová P, Cenklová V, Eder J, Doležel J, Machácková I. Effect of 2-aminoindan-2- phosphonic acid on cell cycle progression in synchronous meristematic cells of Vicia faba roots. Plant Sci 2003;164:823-832.
25. Ochatt SJ, Delaitre C, Lionneton E, Huchette O, Patat-Ochatt EM, Kahane R. One team, PCMV, and one approach, in vino biotechnology, for one aim, the breeding of quality plants with a wide array of species. In: Dris R, editor. Crops Growth, Quality and Biotechnology. Helsinki, Finland: WFL Publ Sci & Technol; 2005. pp 1038-1067. ISBN 952-91-8601-0.
26. Doležel J, Kubaláková M, Paux E, Doležel J, Feuillet C. Chromosome-based genomics in the cereals. Chromosome Res 2007;15:51-66.
27. Ng BL, Yang F, Carter NP. Flow analysis and sorting of microchromosomes (<3 Mb). Cytometry A 2007;71A:410-413.
28. Ng BL, Carter NP. Factors affecting flow karyotype resolution. Cytometry A 2006;69:1028-1036.
29. Doležel J, Lucretti S, Schubert I. Plant chromosome analysis and sorting by flow cytometry. Crit Rev Plant Sci 1994;13:275-309.
30. van Duren M, Morpurgo R, Doležel J, Afza R. Induction and verification of autotetraploids and diploid banana (Musa acuminata) by in vitro techniques. Euphytica 1996;88:25-34.
31. Zonneveld BJM, van Iren F. Genome size and pollen viability as taxonomic criteria: Application to the genus Hosta. Plant Biol 2001;3:176-185.
32. Hao J, You C, Deng X. Cell size as a morphological marker to calculate the mitotic index and ploidy level of citrus callus. Plant Cell Rep 2002;20:1123-1127.
33. Sharma DP, Firoozabady E, Ayres NM, Galbraith DW. Improvement of anther culture in Nicotiana: media, culture conditions and flow cytometric determination of ploidy levels. Int J Plant Physiol 1983;111:441-450.
34. Galbraith DW. Flow cytometric analysis of the cell cycle in higher plants. In: Vasil IK, editor. Cell Culture and Somatic Cell Genetics of Plants. New York: Academic Press; 1984. pp 765-777.
35. De Laat AMM, Göhde W, Vogelzakg MJDC. Determination of ploidy of single plants and plant populations by flow cytometry. Plant Breed 1987;99:303-307.
36. Sree Ramulu K, Dijkhuis P. Flow cytometric analysis of polysomaty and in vitro genetic inestability in potato. Plant Cell Rep 1986;5:234-237.
37. Suda J, Krahulcovà A, Tràvníbek P, Krahulec F. Ploidy level versus DNA ploidy level: An appeal for consistent terminology. Taxon 2006;55:447-450.
38. Suda J, Tràvníbek P. Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry - New prospects for plant research. Cytometry A 2006;69A:273-280.
39. Roberts AV. The use of bead beating to prepare suspensions of nuclei for flow cytometry from fresh leeaves, herbarium leaves, petals and pollen. Cytometry A 2007;71A:1039-1044.
40. Hopping ME. Preparation and preservation of nuclei from plant tissues for quantitative DNA analysis by flow cytometry. N Z J Bot 1993;31:391-401.
41. Chiatante D, Brusa P, Levi M, Sgorbati S, Sparvoli E. A simple protocol to purify fresh nuclei from milligram amounts of meristematic pea root tissue for biochemical and flow cytometry applications. Physiol Plant 1990;78:501-506.
42. Lemontey C, Mousset-Déclas C, Munier-Jolain N, Boutin JP. Maternal genotype influences pea seed size by controlling both mitotic activity during early embryogenesis and final endoreduplication level/cotyledon cell size in mature seeds. J Exp Bot 2000;51:167-175.
43. Ochatt S, Durieu P, Jacas L, Pontécaille C. Protoplast, cell and tissue cultures for the biotechnological breeding of grass peas. Lathyrus Neurolathyr Newslett 2001;2:35-38.
44. Roux N, Doležel J, Swennen R, Zapata-Arias FJ. Effectiveness of three micropropagation techniques to dissociate cytochimeras in Musa spp. Plant Cell Tissue Organ Cult 2001;66:189-197.
45. Roux N, Toloza A, Radecki Z, Zapata-Arias FJ, Doležel J. Rapid detection of aneuploidy in Musa using flow cytometry. Plant Cell Rep 2003;21:483-490.
46. Ozaki Y, Narikiyo K, Fujita C, Okubo H. Ploidy variation of progenies from intra-and inter-ploidy crosses with regard to trisomic production in asparagus (Asparagus officinalis L.). Sex Plant Reprod 2004;17:157-164.
47. Gonzalez Castañón ML, Schroeder MB. Rapid determination of nuclear DNA amounts and ploidy levels in germplasms of Asparagus using flow cytometry. Acta Hort 2002;589:193-199.
48. Barker RE, Kilgore JA, Cook RL, Garay AE, Warnke SE. Use of flow cytometry to determine ploidy level of ryegrass. Seed Sci Technol 2001;29:493-502.
49. Šesek P, Sustar-Vozlic J, Bohanec B. Determination of aneuploids in bop (Humulus lupulus L.) using flow cytometry. Pflügers Archiv - Eur J Physiol 2000;439(Suppl):R16-R18.
50. Pillay M, Ogundiwin E, Tenkouano A, Doležel J. Ploidy and genome composition of Musa germoplasm at the International Institute of Tropical Agriculture (IITA). Afr J Biotechnol 2006;5:1224-1232.
51. Palomino G, Doležel J, Mendez I, Rublou A. Nuclear genome size analysis of Agave tequilana Weber. Caryologia 2003;56:37-46.
52. Brummer EC, Cazcarro PM, Luth D. Ploidy determination of alfalfa germplasm accessions using flow cytometry. Crop Sci 1999;39:1202-1207.
53. Zonneveld BJM. Nuclear DNA contents of all species of Helleborus (Ranunculaceae) discriminate between species and sectional divisions. Plant Systemat Evol 2001;229:125-130.
54. Egesi CN, Pillay M, Asiedu R, Egunjobi JK. Ploidy analysis in water yam, Dioscorea alata L. germplasm. Euphytica 2002;128:225-230.
55. de la Cruz E, Rubluo IA, Palomino HG, Garcia AJM, Laguna CA. Characterization of Chenopodium germplasm, selection of putative mutants and its cytogenetic study. In: Ochatt S, Mohan Jain S, editors. Breeding of Neglected and Under-Utilized Crops, Spices and Herbs. Enfield: Science Publishers; 2007. 447 pp. ISBN 978-1-57808-509-5.
56. Koutoulis A, Roy AT, Price A, Sherriff L, Leggett G. DNA ploidy level of colchicine treated hops (Humulus lupulus L.). Sci Horticult 2005;105:263-268.
57. Thao NTP, Ozaki Y, Okubo H. Colchicine-and oryzalin-induced tetraploids in ornamental Alocasia x amazonica hort. J Japan Soc Horticult Sci 2004;73:63-65.
58. Geoffriau E, Kahane R, Bellamy C, Rancillac M. Ploidy stability and in vitro chromosome doubling in gynogenic clones of onion (Allium cepa L.). Plant Sci 1997;122:201-208.
59. Lionneton E, Beuret W, Delaìtre C, Ochatt S, Rancillac M. Improved microspore culture and double haploid plant regeneration for the brown condiment mustard (Brassica juncea). Plant Cell Rep 2001;20:126-130.
60. Galbraith DW, Harkins KR, Knapp S. Systemic endopolyploidy in Arabidopsis thaliana. Plant Physiol 1991;96:985-989.
61. DeRocher EJ, Harkins KR, Galbraith DW, Bohnert HJ. Developmentally regulated svstemic endopolyploidy in succulents with small genomes. Science 1990;250:99-101.
62. Keeler KH, Kwankin B, Barnes PW, Galbraith DW. Polyploid polymorphism in big bluestern (Andropogon gerardii Vitman). Genome 1987;29:374-379.
63. Gendreau E, Traas J, Desnos T, Grandjean O, Caboche M, Hôfte H. Cellular basis of hypocotyl growth in Arabidopsis thaliana. Plant Physiol 1997;114:295-305.
64. Lee JH, Arumuganathan K, Kaeppler SM, Parl S-W, Kim K-Y, Chung Y-S, Kim D-H, Fukui K. Variability of chromosomal DNA contents in maize (Zea mays L.) inbred and hybrid lines. Planta 2002;215:666-671.
65. Ochatt SJ, Muneaux E, Machado C, Jacas L, Pontécaille C. The hyperhydricity of in vitro regenerants is linked with an abnormal DNA content in grass pea (Lathyrus sativus L.). J Plant Physiol 2002;159:1021-1028.
66. Kopecky D, Loureiro J, Zwierzykowski Z, Ghesquiere M, Doležel J. Genome constitution and evolution in Lolium x Festuca hybrid cultivars (Festulolium). Theor Appl Genet 2006;113:731-742.
67. Loureiro J, Pinto G, Lopes T, Doležel J, Santos C. Assessment of ploidy stability of the somatic embryogenesis process in Quercus suber L. using flow cytometry. Planta 2005;221:815-822.
68. Svirshchevskaya A, Doležel J. Karyological characterization of sugar beet gynogenetic lines cultured in vitro. J Appl Genet 2001;42:21-32.
69. Kopecky D, Lukaszewski AJ, Gibeault V. Reduction of ploidy level by androgenesis in intergeneric Lolium-Festuca hybrids for turf grass breeding. Crop Sci 2005;45:274-281.
70. Ochatt SJ, Pontécaille C, Rancillac M. The growth regulators used for bud regeneration and shoot rooting affect the competence for flowering and seed set in regenerated plants of protein pea. Vitro Cell Dev Biol Plant 2000;36:188-193.
71. Elmaghrabi A, Ochatt S. Isoenzymes and flow cytometry for the assessment off true-to-typeness of calluses and cell suspensions of barrel medic prior to regeneration. Plant Cell Tissue Organ Cult 2006;85:31-43.
72. Sagan M, Morandi D, Tarenghi F, Duc G. Selection of nodulation and mycorrhizal mutants in the model plant Medicago truncatula (Gaertn) after-ray mutagenesis. Plant Sci 1995;111:63-71.
73. Ochatt SJ, Mousset-Declas C, Rancillac M. Fertile pea plants regenerate from protoplasts when calluses have not undergone endoreduplication. Plant Sci 2000;156:177-183.
74. Tadege M, Ratet P, Mysore KS. Insertional mutagenesis: A Swiss Army knife for functional genomics of Medicago truncatula. Trends Plant Sci 2005;10:229-235.
75. Grandbastien M-A, Audeon C, Bonnivard E, Casacuberta JM, Chalhoub B, Costa APP, Le QH, Melayah D, Petit M, Poncet C, Tam S-M, van Sluys M-A, Mhiri C. Stress activation and genomic impact of Tnt1 retrotransposons in Solanaceae. Cytogenet Genome Res 2005;110:229-241.
76. Iantcheva A, Vlahova M, Trinh TH, Brown SC, Slater A, Elliott MC, Atanassov A. Assessment of polysomaty, embryo formation and regeneration in liquid media for various species of diploid annual Medicago. Plant Sci 2001;160:621-627.
77. Doležel J, Göhde W. Sex determination in dioecious plants Melandrium album and M. rubrum using high-resolution flow cytometry. Cytometry 1995;19:103-106.
78. Delaitre C, Ochatt SJ, Deleury E. Electroporation modulates the embryogenic responses of asparagus (Asparagus officinalis L.) microspores. Protoplasma 2001;216:39-46.
79. Delaitre C, Ochatt S. Electric fields plus growth regulators foster embryogenesis and androgenic plant regeneration in Asparagus officinalis. Acta Hort, in press.
80. Sharbel TF, Voigt ML, Mitchell-Olds T, Kantama L, de long H. Is the aneuploid chromosome in an apomictic Boechera holboellii a genuine B chromosome? Cytogenet Genome Res 2004;106:173-183.
81. Leitch IJ, Bennett MD. Genome size and its uses: The impact of flow cytometry. In: Greilhuber J, Suda J, editors. Flow Cytometry With Plant Cells. Analysis of Genes, Chromosomes and Genomes. Weinheim: Wiley-VCH; 2007. pp 153-176. KgaA, ISBN: 978-3-527-31487-4.
82. Arumuganathan K, Earle ED. Nuclear DNA content of some important plant species. Plant Mol Biol Rep 1991;9:208-218.
83. Greilhuber J, Doležel J, Lysak MA, Bennett MD. The origin, evolution and proposed stabilization of the terms 'genome size' and 'C-value' to describe nuclear DNA contents. Ann Bot 2005;95:255-260.
84. Narayan RKJ. Discontinuous DNA variation in the evolution of plant species - The genus Lathyrus. Evolution 1982;36:877-891.
85. Ali HBM, Meister A, Schubert I. DNA content, rDNA loci, and DAPI bands reflect the phylogenetic distance between Lathyrus species. Genome 2000;43:1027-1032.
86. Hutchinson J, Narayan RKJ, Rees H. Constraints upon the composition of supplementary DNA. Chromosoma 1980;78:137-145.
87. Nandini AV, Murray BG, O'Brien IEW, Hammett KRW. Intra- and interspecific variation in genome size in Lathyrus (Leguminosae). Bot J Linnean Soc 1997;125:359-366.
88. Walker DJ, Monino I, Correal E. Genome size in Bituminaria bituminosa (L.) C.H. Stirton (Fabaceae) populations: Separation of "true" differences from environmental effects on DNA determination. Environm Exp Bot 2006;55:258-265.
89. Meister A, Barow M. DNA base composition of plant genomes. In: Greilhuber J, Suda J, editors. Flow Cytometry with Plant Cells. Analysis of Genes, Chromosomes and Genomes. Weinheim: Wiley-VCH; 2007. pp 177-215. KgaA, ISBN: 978-3-527-31487-4.
90. Doležel J, Greilhuber J, Lucretti S. Plant genome size estimation: Inter-laboratory comparison. Ann Bot 1998;82(Suppl A):17-26.
91. Doležel J, Bartoš J. Plant DNA flow cytometry and estimation of nuclear genome size. Ann Bot 2005;95:99-110.
92. Doležel J, Kubaláková M, Bartoš J, Macas J. Flow cytogenetics and plant genome mapping. Chromosome Res 2004;12:77-91.
93. Uberall I, Vrana J, Doležel J, Smerda J, Bartoš J, Havel L. Isolation of chromosomes from Picea abies and their analysis by flow cytometry. Biol Plantarum 2004;48:199-203.
94. Kováiová P, Navrátilová A, Macas J, Doležel J. Chromosome analysis and sorting in Vicia sativa using flow cytometry. Biol Plantarum 2007;51:43-48.
95. Neumann P, Pozárkova D, Vrána J, Doležel J, Macas J. Chromosome sorting and PCR-based physical mapping in pea (Pisum sativum L). Chromosome Res 2002;10:63-71.
96. Vlácilová K, Ohri D, Vrána J, Jíhalí ková J, Kahl G, Doležel J. Development of flow cytogenetics and physical genome mapping in chickpea (Cicer arietinum L.). Chromosome Res 2002;10:695-706.
97. Doležel J, Lucretti S. High-resolution flow karyotyping and chromosome sorting in Vicia faba lines with standard and reconstructed karyotypes. Theor Appl Genet 1995;90:797-802.
98. Kubaláková M, Valárik M, Bartoš J, Vrána J, Cíhalíková J, Molnar-Lang M, Doležel J. Analysis and sorting of rye (Secale cereale L.) chromosomes using flow cytometry. Genome 2003;46:893-905.
99. Kubaláková M, Lysák MA, Vrána J, Simková H, Cíhalíková J, Doležel J. Rapid identification and determination of purity of flow-sorted plant chromosomes using C-PRINS. Cytometry 2000;41:102-108.
100. Suchánková P, Kubaláková M, Kovárová P, Bartoš J, Cíhalíková J, Molnar-Lang M, Takashi R, Endo, Doležel J. Dissection of the nuclear genome of barley by chromosome flow sorting. Theor Appl Genet 2006;113:651-659.
101. Kubaláková M, Kovárová P, Suchánková P, Cíhalíková J, Bartoš J, Lucretti S, Watanabe N, Kianian SF, Doležel J. Chromosome sorting in tetraploid wheat and its potential for genome analysis. Genetics 2005;170:823-829.
102. Li L, Arumuganathan K. Physical mapping of 45S and 5S rDNA on maize metaphase and sorted chromosomes by FISH. Hereditas 2001;134:141-145.
103. Meyers BC, Scalahrin S, Morgante M. Mapping and sequencing complex genomes: Let's get physical! Nature Rev Genet 2004;5:578-581.
104. Luo MC, Thomas C, You FM, Hsiao J, Ouyang S, Buell CR, Malandro M, McGuire PE, Anderson OD, Dvorak J. High-throughput fingerprinting of bacterial artificial chromosomes using the Snapshot labelling kit and sizing of restriction fragments by capillary electrophoresis. Genomics 2003;82:378-389.
105. Benabdelmouna A, Shi Y, A-Darmency M, Darmency H. Genomic in situ hybridization (GISH) discriminates between the A and the B genomes in diploid and tetraploid Setaria species. Genome 2001;44:685-690.
106. Benabdelmouna A, A-Darmency M, Darmency H. Phylogenetic and genomic relationships in Setaria italica and its close relatives based on the molecular diversity and chromosomal organization of 5S and 18S.-5.8S -25S rDNA genes. Theor Appl Genet 2001;103:668-677.
107. Darmency AM, Chelysheva L, Prado E. Variation in rDNA locus number and position amongst legume species and its implication for the dynamics of genome organisation. Genome 2005;48:556-561.
108. Ochatt SJ, Abirached-Darmency M, Marget P, Aubert G. The Lathyrus paradox: "poor men's diet" or a remarkahle genetic resource for protein legume breeding? In: Ochatt SJ, Jain SM, editors. Breeding of Neglected and Under-utilised Crops, Spices And Herbs. Plymouth, UK: Science Press; 2007. pp 41-60. ISBN 978-1-57808-0.
109. Kubalàkovà M, Vràna J, Cíhalí ková J, Lysàk MA, Doležel J. Localization of DNA sequences on plant chromosomes using PRINS and C-PRINS. Methods Cell Sci 2001;23:71-82.
110. Roy AT, Leggett G, Koutoulis A. In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.). Plant Cell Rep 2001;20:489-495.
111. Eeckhant T, Leus L, van Huylenbroeck J. Exploitation of flow cytometry for plant breeding. Acta Physiol Plant 2006;27:743-750.
112. Zonneveld BJM, van Iren F. Flow cytometric analysis of DNA content in Hosta reveals ploidy chimeras. Euphytica 2000;111:105-110.
113. Galbraith DW. Protoplast analysis using flow cytometry and sorting. In: Doležel J, Greilhuber J, Suda J, editors. Flow Cytometry with Plant Cells. Analysis of Genes, Chromosomes and Genomes. Weinheim: Wiley-VCH; 2007. pp 231-250. KgaA, ISBN: 978-3-527-31487-4.
114. Galbraith DW, Harkins KR, Jefferson RA. Flow cytometric characterization of the chlorophyll contents and size distribution of plant protoplasts. Cytometry 1988;9:75-83.
115. Galbraith DW, Shields BS. The effects of inhibitors of cell wall synthesis on tobacco protoplast development. Physiol Plant 1982;55:25-30.
116. Gantet P, Hubac C, Brown SC. Flow cytometric fluorescence anisotropy of lipophilic probes in epidermal and mesophyll protoplasts from water-stressed Lupinus albus L. Plant Physiol 1990;94:729-737.
117. Lei XY, Liao XD, Zhang GY, Dai YR. Flow cytometric evidence for hydroxyl radical induced apoptosis in tobacco protoplasts. Acta Bot Sin 2003;45:944-948.
118. Weir IE, Pham N-A, Hedley DW. Oxidative stress is generated via the mitochondrial respiratory chain during plant cell apoptosis. Cytometry 2003;54:109-117.
119. Cronje MJ, Weir IE, Bornmann L Salicylic acid-mediated potentiation of Hsp70 induction correlates with reduced apoptosis in tobacco protoplasts. Cytometry A 2004;61A:76-87.
120. Brown S, Renaudin JP, Prevot C, Guern J. Flow cytometry and sorting of plant protoplasts-technical problems and physiological results from a study of pH and alkaloids in Catharanthus roseus. Physiol Veg 1984;22:541-554.
121. Yanpaisan W, King NJC, Doran PM. Flow cytometry of plant cells with applications in large-scale bioprocessing. Biotechnol Adv 1999;17:3-27.
122. Hall RD, Yeoman MM. Intercellular and intercultural heterogeneity in secondary metabolite accumulation in cultures of Catharanthus roseus following cell line selection. J Exp Bot 1987;38:1391-1398.
123. Hara Y, Yamagata H, Morimoto T, Hiratsuka J, Yoshioka T, Fujita Y, Yamada Y. Flow cytometric analysis of cellular berberine contents in high- and low-producing cell lines of Coptis japonica obtained by repeated selection. Planta Med 1989;2:151-154.
124. Sakamoto K, Iida K, Koyano T, Asada Y, Furuya T. Method for selecting anthocyanin-producing cells by a cell sorter. Planta Med 1994;60:253-259.
125. Afonso CL, Harkins KR, Thomas-Compton M, Kejci A, Galbraith DW. Production of somatic hybrid plants through fluorescence activated sorting of protoplasts. Biotechnology 1985;3:811-816.
126. Fahleson I, Råhlén L, Glimelius K. Analysis of plants regenerated from protoplast fusions between Brassica napus and Eruca sativa.. Theor Appl Genet 1988;76:507-512.
127. Waara S, Nyman M, Johannisson A. Efficient selection of potato heterokaryons by flow cytometric sorting and the regeneration of hybrid plants. Euphytica 1998;101:293-299.
128. Durieu P, Ochatt SJ. Efficient intergeneric fusion of pea (Pisum sativum L.) and grass pea (Lathyrus sativus L.) protoplasts. J Exp Bot 2000;51:1237-1242.
129. Geerts P, Druart PH, Ochatt S, Baudoin J-P. Protoplast fusion for Phaseolus breeding. BASE 2008;12:41-46.
130. Pâques M, Strasser R, Ochatt S, Guinchard A, Auderset G, Moncousin CH. A biological test to evaluate, in 10 days, chemicals to prevent microcutting hyperhydricity. COST843 Final Conference. Nitra, Slovak Republic: Institute of Plant Genetics and Biotechnology, Slovak Academy of Science; 2005. pp 108-109. ISBN 80-890288-41-4.
131. Duc G, Messager A. Mutagenesis of pea (Pisum sativum L.) and isolation of mutants for nodulation and nitrogen fixation. Plant Sci 1989;60:207-213.
132. Gallardo K, Kurt C, Thompson R, Ochatt S. In vitro culture of immature M. truncatula grains under conditions permitting embryo development comparable to that observed in vivo. Plant Sci 2006;170:1052-1058.
133. Schweizer L, Yerk-Davis GL, Phillips RL, Srienc F, Jones RJ. Dynamics of maize endosperm development and DNA endoreduplication. PNAS 1995;18:7070-7074.
134. Engelen-Eigles G, Jones RJ, Phillips RL. DNA endoreduplication in maize endosperm cells is reduced by high temperature during the mitotic phase. Crops Sci 2001;41:1114-1121.
135. Kowles RV, Srienc F, Phillips RL. Endoreduplication of nuclear DNA in the developing maize endosperm. Dev Genet 2005;11:125-132.
136. Artlip TS, Madison JT, Setter TL. Water deficit in developing endosperm of maize: Cell division and nuclear DNA endoreduplication. Plant Cell Environ 1995;18:1034-1040.
137. Mambelli S, Setter TL. Inhibition of maize endosperm cell division and endoreduplication by exogenously applied abscisic acid. Physiol Plant 1998;104:266-272.
138. Ashcroft RG, Preston C, Cleland RE, Critchley C. Flow cytometry of isolated chloroplasts and thylakoids. Photobiochem Photobiophys 1986;13:1-14.
139. Schroeder WP, Petit PX. Flow cytometry of spinach chloroplasts: Determination of intactness and lectin-binding properties of the envelope and the thylakoid membranes. Plant Physiol 1992;100:1092-1102.
140. Kausch AP, Bruce BD. Isolation and immobilization of various plastid subtypes by magnetic immunoabsorption. Plant J 1994;6:767-779.
141. Petit PX, Diolez P, Muller P, Brown SC. Binding of concanavalin A to the outer membrane of potato tuber mitochondria detected by flow cytometry. FEBS Lett 1986;196:65-70.
142. Petit PX. Flow cytometric analysis of rhodamine 123 fluorescence during modulation of the membrane potential in plant mitochondria. Plant Physiol 1992;98:279-286.
143. Ammirato PV. Embryogenesis. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y, editors. Handbook of Plant Cell Culture, Vol. 1: Techniques for Propagation and Breeding. New York: Mac Millan; 1983. pp 82-123.
144. Sugimoto K, Williamson RE, Wasteneys GO. Wall architecture in the cellulose deficient rswI mutant of Arabidopsis thaliana: Microfibrils but not microtubules lose their transverse alignment before microfibrils become unrecognisable in the mitotic and elongation zones of roots. Protoplasma 2001;215:172-183.
145. Ochatt S, Muilu R, Ribalta F. Early indicators of onset of embryogenesis from cell suspension cultures. Plant Biosystems 2008;142: in press.
146. Verbelen JP, Kerstens S. Polarization confocal microscopy and Congo red fluorescence: A simple and rapid method to determine the mean cellulose fibril orientation in plants. J Microsc 2000;198:101-107.
147. Vissenberg K, Martinez-Vilchez IM, Verbelen JP, Miller J, Fry SC. In vivo colocalization of xyloglucan endotransglycosylase activity and its donor substrate in the elongation zone of Arabidopsis roots. Plant Cell 2000;12:1229-1237.
148. Fry SC, Smith RC, Renwick KF, Martin DJ, Hodge SK, Matthews KJ. Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from plants. Biochem J 1992;282:821-828.
149. Trinh TH, Ratet P, Kondorosi E, Durand P, Kamaté K, Bauer P, Kondorosi A. Rapid and efficient transformation of diploid Medicago truncatula and Medicago sativa ssp. Falcata lines improved in somatic embryogenesis. Plant Cell Rep 1998;17:345-355.
150. Ulrich W. Simultaneous measurement of dapi-sulforhodamine 101 stained nuclear DNA and protein in higher plants by flow cytometry Biotech Histochem 1992;67:73-78.
151. Zubkov MV, Burkill PH. Syringe pumped high speed flow cytometry of oceanic phytoplankton. Cytometry A 2006;69A:273-280.