Fluorescence spectra of Trp53Phe and Trp110Ile mutants of a heme-regulated phosphodiesterase from Escherichia coli

Chemistry Letters

Volumn 33, Issue 7, 2004, Pages 870-871

  Hirata, Satoshi ^a   Kurokawa, Hirofumi ^a   Sagami, Ikuko ^a   Shimizu, Toru ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

8 ANILINO 1 NAPHTHALENESULFONIC ACID; HEME; ISOLEUCINE; PHENYLALANINE; PHOSPHODIESTERASE; TRYPTOPHAN;

ARTICLE; ESCHERICHIA COLI; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; PHOTOCHEMICAL QUENCHING; PROTEIN ISOLATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; SPECIES COMPARISON; WILD TYPE;

EID: 4644373192     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2004.870     Document Type: Article

References (16)

1. V. M. Delgado-Nixon, G. Gonzalez, and M. A. Gilles-Gonzalez, Biochemistry, 39, 2685 (2000).
2. Y. Sasakura, S. Hirata, S. Sugiyama, S. Suzuki, S. Taguchi, M. Watanabe, T. Matsui, I. Sagami, and T. Shimizu, J. Biol. Chem., 277, 23821 (2002).
3. A. Sato, Y. Sasakura, S. Sugiyama, I. Sagami, T. Shimizu, Y. Mizutani, and T. Kitagawa, J. Biol. Chem., 277, 32650 (2002).
4. T. Yoshimura, I. Sagami, Y. Sasakura, and T. Shimizu, J. Biol. Chem., 278, 53105 (2003).
5. S. Taguchi, T. Matsui, J. Igarashi, Y. Sasakura, Y. Araki, O. Ito, S. Sugiyama, I. Sagami, and T. Shimizu, J. Biol. Chem., 279, 3340 (2004).
6. W. Gong, B. Hao, S. S. Mansy, G. Gonzalez, M. A. Gilles-Gonzalez, and M. K. Chan, Proc. Natl. Acad. Sci. U.S.A., 95, 15177 (1998).
7. G. E. Borgstahl, D. R. Williams, and E. D. Getzoff, Biochemistry, 34, 6278 (1995).
8. E. Soderback, F. Reyes-Ramirez, T. Eydmann, S. Austin, S. Hill, and R. Dixon, Mol. Microbiol., 28, 179 (1998).
9. "Principles of Fluorescence Spectroscopy," J. Lackowicz, Plenum Press, New York (1983).
10. Z. Gryczynski, J. Lubkowski, and E. Bucci, Methods Enzymol., 278, 538 (1997).
11. H. Miyatake, M. Mukai, S. Y. Park, S. Adachi, K. Tamura, H. Nakamura, K. Nakamura, T. Tsuchiya, T. Iizuka, and Y. Shiro, J. Mol. Biol., 301, 415 (2000).
12. H. Kurokawa, D. S. Lee, M. Watanabe, I. Sagami, B. Mikami, C. S. Raman, and T. Shimizu, J. Biol. Chem., 279, 20186 (2004).
13. P. J. Kraulis, J. Appl. Crystallogr., 24, 946 (1991)
14. E. A. Merrit and D. J. Bacon, Methods Enzymol., 277, 505 (1997).
15. Angle between the heme plane and the side-chain of Trp53 is approximately 50°.
16. Note that the role Trp110 stated here is only a proposal. To verify the proposal, further experiments are needed.