Isoosmotic isolation of rat brain synaptic vesicles, some of which contain tyrosine hydroxylase

Journal of Biochemistry

Volumn 136, Issue 2, 2004, Pages 239-243

  Tsudzuki, Toshihiro ^a   Tsujita, Maki ^a  

^a NAGOYA CITY UNIV MEDICAL SCHOOL   (Japan)

Author keywords

H+ ATPase; Synaptic vesicles; Tyrosine hydroxylase

Indexed keywords

PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE; TYROSINE 3 MONOOXYGENASE; VESICULAR ACETYLCHOLINE TRANSPORTER;

ALPHA CHAIN; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CALCIUM TRANSPORT; CATECHOLAMINE SYNTHESIS; CELL FRACTIONATION; CELL ISOLATION; CONTROLLED STUDY; CYTOSOL; IMMUNOBLOTTING; MALE; NERVE ENDING; NERVE STIMULATION; NEUROTRANSMITTER RELEASE; NONHUMAN; OSMOSIS; OSMOTIC STRESS; RAT; SYNAPSE VESICLE; SYNAPTOSOME;

ACETYLCHOLINE; ADENOSINE TRIPHOSPHATASES; ANIMALS; BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; BRAIN; CA(2+) MG(2+)-ATPASE; CHROMATOGRAPHY, GEL; CYTOSOL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MALE; MICROSCOPY, ELECTRON; OSMOSIS; RATS; RATS, WISTAR; SUCROSE; SYNAPSES; SYNAPTIC VESICLES; SYNAPTOSOMES; TYROSINE 3-MONOOXYGENASE;

ANIMALIA;

EID: 4644367206     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvh113     Document Type: Article

References (27)

1. Whittaker, V.P. (1969) The Synaptosome in Handbook of Neurochemistry (Lajtha, A. ed.) Vol. II, pp. 327-364, Plenum Press, Yew York and London
2. +-ATPase in brain synaptic vesicles. J. Biochem. 108, 689-693
3. 1. Biochem. Biophys. Res. Commun. 170, 873-878
4. Yamagata, S.K. and Parsons, S.M. (1989) Cholinergic synaptic vesicles contain a V-type and a P-type ATPase. J. Neurochem. 53, 1354-1362
5. Tsudzuki, T. (2003) Isolation of small agranular synaptic vesicles of rat brain by gel filtration chromatography. J. Biochem. 133, 437-443
6. Nagatsu, T., Levitt, M., and Udenfriend, S. (1964) Tyrosine hydroxylase: the initial step in norepinephrine biosynthesis. J. Biol. Chem. 239, 2910-2917
7. Nagatsu, T. and Nagatsu, I. (1970) Subcellular distribution of tyrosine hydroxylase and monoamine oxidase in the bovine caudate nucleus. Experientia 26, 722-723
8. Haycock, J.W. (1991) Four forms of tyrosine hydroxylase are present in human adrenal medulla. J. Neurochem. 56, 2139-2142
9. Weihe, E., Tao-Cheng, J.H., Schafer, M.K.-H., Erickson, J.D., and Eiden, L.E. (1996) Visualization of the vesicular acetylcholine transpoter in cholinergic nerve terminals and its targeting to a specific population of small synaptic vesicles. Proc. Natl Acad. Sci. USA 93, 3547-3552
10. Nirenberg, M.J., Liu, Y., Peter, D., Edwards, R.H., and Pickel, V.M. (1995) The vesicular monoamine transporter 2 is present in small synaptic vesicles and preferentially localizes to large dense core vesicles in rat solitary tract nuclei. Proc. Natl Acad. Sci. USA 92, 8773-8777
11. Moriyama, Y. and Yamamoto, A. (1995) Mirovesicles isolated from bovine pineal gland specifically accumulate L-glutamate. FEBS Lett. 367, 233-236
12. Gray, E.G. and Whittaker, V.P. (1962) The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. J. Anat. (Lond.) 96, 79-88
13. 2+-dependent adenosine triphosphatase in isolated synaptic vesicles: Attribution of most of the enzymic activity to an actomyosin-like protein. J. Biochem. 85, 567-574
14. Martin, J.B. and Doty, D.M. (1949) Determination of inorganic phosphate: Modification of isobutyl alcohol procedure. Anal. Chem. 21, 965-967
15. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the Folin-phenol reagent. J. Biol. Chem. 193, 265-275
16. Kadota, K. and Kadota, T. (1973) Isolation of coated vesicles, plain synaptic vesicles, and flocculent material from a crude synaptosome fraction of guinea pig whole brain. J. Cell Biol. 58, 135-151
17. Nelson, N. (1992) Evolution of organellar proton-ATPases. Biochim. Biophys. Acta 1100, 109-124
18. Moriyama, Y. and Nelson, N. (1987) The purified ATPase from chromaffin granule membranes is an anion-dependent proton pump. J. Biol. Chem. 262, 9175-9180
19. +-ATPase domains are transported separately in axon and assemble in Torpedo nerve endings. J. Neurochem. 71, 1702-1708
20. +-ATPase complex. Biochim. Biophys. Acta 980, 241-247
21. 2+/calmodulin-dependent protein kinase II: Effect of an activator protein. J. Neurochem. 49, 1241-1249
22. Haycock, J.W. and Haycock, D.A. (1991) Tyrosine hydroxylase in rat brain dopaminergic nerve terminals: multiple-site phosphorylation in vivo and in synaptosomes. J. Biol. Chem. 288, 5650-5657
23. Haycock, J.W. and Wakade, A.R. (1992) Activation and multiple-site phosphorylation of tyrosine hydroxylase in perfused rat adrenal glands. J. Neurochem. 58, 57-64
24. Ben-Jonathan, N., Maxson, R.E., and Ochs, S. (1978) Fast axoplasmic transport of noradrenaline and dopamine in mammalian peripheral nerve. J. Physiol. (Lond.) 281, 315-324
25. Haggendal, J., Dahlstrom, A., and Larsson, P.A. (1975) Rapid transport of noradrenaline in adrenergic axons of rat sciatic nerve distal to a crush. Acta Physiol. Scand. 94, 386-392
26. Lander, J. and Austin, L. (1976) Subcellular distribution of dopamine-β-hydroxylase and inhibitors in the hippocampus and caudate nucleus in sheep brain. J. Neurochem. 28, 661-673
27. Nagy, A., Varady, G., Joo, F., Rakonczay, Z., and Pilc, A. (1977) Separation of acetylcholine and catecholamine containing synaptic vesicles from brain cortex. J. Neurochem. 29, 449-459