Detection of intrahepatic hepatitis B virus DNA and correlation with hepatic necroinflammation and fibrosis

Journal of Clinical Microbiology

Volumn 42, Issue 9, 2004, Pages 3920-3924

  Wong, Danny Ka Ho ^a   Yuen, Man Fung ^a   Tse, Eric ^a   Yuan, HeJun ^a,b   Sum, Simon Siu Man ^a   Hui, Chee Kin ^a   Lai, Ching Lung ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

^b FUDAN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

HEPATITIS B(E) ANTIBODY; HEPATITIS B(E) ANTIGEN; VIRUS DNA;

ADULT; ARTICLE; DIAGNOSTIC TEST; DNA DETERMINATION; FEMALE; HEPATITIS B; HEPATITIS B VIRUS; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; LIVER FIBROSIS; LIVER NECROSIS; MAJOR CLINICAL STUDY; MALE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VIRUS DETECTION;

ACTINS; ADULT; BASE SEQUENCE; DNA PRIMERS; DNA, VIRAL; FEMALE; HEPATITIS B; HEPATITIS B E ANTIGENS; HEPATITIS B VIRUS; HUMANS; INFLAMMATION; LIVER; LIVER CIRRHOSIS; MALE; POLYMERASE CHAIN REACTION;

DNA VIRUSES; HEPATITIS B VIRUS;

EID: 4644354059     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.9.3920-3924.2004     Document Type: Article

References (21)

1. Brechtbuehl, K., S. A. Whalley, G. M. Dusheiko, and N. A. Saunders. 2001. A rapid real-time quantitative polymerase chain reaction for hepatitis B virus. J. Virol. Methods 93:105-113.
2. Cacciola, I., T. Pollicino, G. Squadrito, G. Cerenzia, D. Villari, R. de Franchis, T. Santantonio, S. Brancatelli, G. Colucci, and G. Raimondo. 2000. Quantification of intrahepatic hepatitis B virus (HBV) DNA in patients with chronic HBV infection. Hepatology 31:507-512.
3. Chen, R. W., H. Piiparinen, M. Seppanen, P. Koskela, S. Santa, and M. Lappalainen. 2001. Real-time PCR for detection and quantitation of hepatitis B virus DNA. J. Med. Virol. 65:250-256.
4. Chu, C. J., M. Hussain, and A. S. Lot 2002. Quantitative serum HBV DNA levels during different stages of chronic hepatitis B infection. Hepatology 36:1408-1415.
5. Gunther, S., L. Fischer, I. Pult, M. Sterneck, and H. Will. 1999. Naturally occurring variants of hepatitis B virus. Adv. Virus Res. 52:25-137.
6. Hsu, Y. S., R. N. Chien, C. T. Yeh, I. S. Sheen, H. Y. Chiou, C. M. Chu, and Y. F. Liaw. 2002. Long-term outcome after spontaneous HBeAg seroconversion in patients with chronic hepatitis B. Hepatology 35:1522-1527.
7. Josefsson, A. M., P. K. Magnusson, N. Ylitalo, P. Sorensen, P. Qwarforth-Tubbin, P. K. Andersen, M. Melbye, H. O. Adami, and U. B. Gyllensten. 2000. Viral load of human papilloma virus 16 as a determinant for development of cervical carcinoma in situ: a nested case-control study. Lancet 355:2189-2193.
8. Knodell, K. G., K. G. Ishak, W. C. Black, T. S. Chen, K. Craig, N. Kaplowitz, T. W. Kiernan, and J. Wollman. 1981. Formulation and application of a numerical scoring system for assessing histological activity in asymptomatic chronic active hepatitis. Hepatology 1:431-435.
9. Lai, C. L., C. K. Ching, A. K. Tung, E. Li, J. Young, A. Hill, B. C. Wong, J. Dent, and P. C. Wu, 1997. Lamivudine is effective in suppressing hepatitis B virus DNA in Chinese hepatitis B surface antigen carriers: a placebo-controlled trial. Hepatology 25:241-244.
10. Lok, A. S., E. J. Heathcote, and J. H. Hoofnagle. 2001. Management of hepatitis B: 2000-summary of a workshop. Gastroenterology 120:1828-1853.
11. Lopez, V. A., E. J. Bourne, M. W. Lutz, and L. D. Condreay. 2002. Assessment of the COBAS Amplicor HBV Monitor test for quantitation of serum hepatitis B virus DNA levels. J. Clin. Microbiol. 40:1972-1976.
12. Moraleda, G., J. Saputelli, C. E. Aldrich, D. Averett, L. Condreay, and W. S. Mason. 1997. Lack of effect of antiviral therapy in nondividing hepatocyte cultures on the closed circular DNA of woodchuck hepatitis virus. J. Virol. 71:9392-9399.
13. Nakajima-Iijima, S., H. Hamada, P. Reddy, and T. Kakunaga. 1985. Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns. Proc. Natl. Acad. Sci. USA 82:6133-6137.
14. Noborg, U., A. Gusdal, E. K. Pisa, A. Hedrum, and M. Lindh. 1999. Automated quantitative analysis of hepatitis B virus DNA by using the Cobas Amplicor HBV Monitor test. J. Clin. Microbiol. 37:2793-2797.
15. Sturzenbaum, S. R., and P. Kille. 2001. Control genes in quantitative molecular biological techniques: the variability of invariance. Comp. Biochem. Physiol. Part B Biochem. Mol. Biol. 130:281-289.
16. Suzuki, T., P. J. Higgins, and D. R. Crawford. 2000. Control selection for RNA quantitation. BioTechniques 29:332-337.
17. Weinberger, K. M., E. Wiedenmann, S. Bohm, and W. Jilg. 2000. Sensitive and accurate quantitation of hepatitis B virus DNA using a kinetic fluorescence detection system (TaqMan PCR). J. Virol. Methods 85:75-82.
18. Yuen, M. F., and C. L. Lai. 1999. Debates in hepatitis: how to assess HBV DNA reductions in association with therapy. Viral Hepatitis Rev. 5:159-175.
19. Yuen, M. F., and C. L. Lai. 2000. Natural history of chronic hepatitis B virus infection. J. Gastroenterol. Hepatol. 15(Suppl.):E20-E24.
20. Yuen, M. F., E. Sablon, H. J. Yuan, C. K. Hui, D. K.-H. Wong, J. Doutreloigne, B. C.-Y. Wong, A. O.-O. Chan, and C. L. Lai. 2002. Relationship between the development of precore and core promoter mutations and hepatitis B e antigen seroconversion in patients with chronic hepatitis B virus. J. Infect. Dis. 186:1335-1338.
21. Yuen, M. F., H. J. Yuan, C. K. Hui, D. K.-H. Wong, W. M. Wong, A. O.-O. Chan, B. C.-Y. Wong, and C L. Lai. 2003. A large population study of spontaneous HBeAg seroconversion and acute exacerbation of chronic hepatitis B infection: implications for antiviral therapy. Gut 52:416-419.