Structural and functional analysis of the C-terminal cytoplasmic domain of FlhA, an integral membrane component of the type III flagellar protein export apparatus in Salmonella

Journal of Molecular Biology

Volumn 343, Issue 2, 2004, Pages 457-466

  Saijo Hamano, Yumiko ^a   Minamino, Tohru ^a   MacNab, Robert M ^b   Namba, Keiichi ^a,c  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b YALE UNIVERSITY   (United States)

^c OSAKA UNIVERSITY   (Japan)

Author keywords

export apparatus; flagella; Salmonella; temperature sensitive mutant; type III protein export

Indexed keywords

AMINO ACID; BACTERIAL PROTEIN; MEMBRANE PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CELL MOTILITY; CYTOPLASM; DNA SEQUENCE; GEL FILTRATION CHROMATOGRAPHY; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN SECRETION; PROTEIN STRUCTURE; SALMONELLA; TEMPERATURE SENSITIVITY; ULTRACENTRIFUGATION; ULTRAVIOLET SPECTROSCOPY; WILD TYPE;

SALMONELLA;

EID: 4644346442     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2004.08.067     Document Type: Article

References (30)

1. R.M. Macnab Flagella and motility F.C. Neidhardt R. Curtiss III J.L. Ingraham E.C.C. Lin K.B. Low B. Magasanik Escherichia coli and Salmonella: Cellular and Molecular Biology 2nd edit. 1996 ASM Press Washington, DC 123 145
2. R.M. Macnab How bacteria assemble flagella Annu. Rev. Microbiol. 57 2003 77 100
3. T. Minamino, and K. Namba Self-assembly and type III protein export of the bacterial flagellum J. Mol. Microbiol. Biotechnol. 7 2004 5 17
4. T. Minamino, and R.M. Macnab Components of the Salmonella flagellar export apparatus and classification of export substrates J. Bacteriol. 181 1999 1388 1394
5. T. Minamino, and R.M. Macnab Interactions among components of the Salmonella flagellar export apparatus and its substrates Mol. Microbiol. 35 2000 1052 1064
6. F. Fan, K. Ohnishi, N.R. Francis, and R.M. Macnab The FliP and FliR proteins of Salmonella typhimurium, putative components of the type III flagellar export apparatus, are located in the flagellar basal body Mol. Microbiol. 26 1997 1035 1046
7. H. Suzuki, K. Yonekura, K. Murata, T. Hirai, K. Oosawa, and K. Namba A structural feature in the central channel of the bacterial flagellar FliF ring complex is implicated in type III protein export J. Struct. Biol. 124 1998 104 114
8. M. Kihara, T. Minamino, S. Yamaguchi, and R.M. Macnab Intergenic suppression between the flagellar MS ring protein FliF of Salmonella and FlhA, a membrane component of its export apparatus J. Bacteriol. 183 2001 1655 1662
9. N.R. Francis, G.E. Sosinsky, D. Thomas, and D.J. DeRosier Isolation, characterization, and structure of bacterial flagellar motors containing the switch complex J. Mol. Biol. 235 1994 1261 1270
10. H. Suzuki, K. Yonekura, and K. Namba Structure of the rotor of the bacterial flagellar motor revealed by electron cryomicroscopy and single particle image analysis J. Mol. Biol. 337 2004 105 113
11. 1 ATPase and to virulence proteins of mammalian and plant pathogens J. Bacteriol. 175 1993 3131 3138
12. F. Fan, and R.M. Macnab Enzymatic characterization of FliI: an ATPase involved in flagellar assembly in Salmonella typhimurium J. Biol. Chem. 271 1996 31981 31988
13. E. Silva-Herzog, and G. Dreyfus Interaction of FliI, a component of the flagellar export apparatus, with flagellin and hook protein Biochim. Biophys. Acta 1431 1999 374 383
14. F. Auvray, A.J. Ozin, L. Claret, and C. Hughes Intrinsic membrane targeting of the flagellar export ATPase FliI: interaction with acidic phospholipids and FliH J. Mol. Biol. 318 2002 941 950
15. B. González-Pedrajo, G.M. Fraser, T. Minamino, and R.M. Macnab Molecular dissection of Salmonella FliH, a regulator of the ATPase FliI and the type III flagellar protein export pathway Mol. Microbiol. 45 2002 967 982
16. T. Minamino, and R.M. Macnab FliH, a soluble component of the type III flagellar export apparatus of Salmonella, forms a complex with FliI and inhibits its ATPase activity Mol. Microbiol. 37 2000 1494 1503
17. T. Minamino, B. González-Pedrajo, M. Kihara, K. Namba, and R.M. Macnab The ATPase FliI can interact with the type III flagellar protein export apparatus in the absence of its regulator FliH J. Bacteriol. 185 2003 3983 3988
18. T. Minamino, R. Chu, S. Yamaguchi, and R.M. Macnab Role of FliJ in flagellar protein export in Salmonella J. Bacteriol. 182 2000 4207 4215
19. T. Minamino, T. Iino, and K. Kutsukake Molecular characterization of the Salmonella typhimurium flhB operon and its protein products J. Bacteriol. 176 1994 7630 7637
20. G.M. Fraser, B. González-Pedrajo, J.R.H. Tame, and R.M. Macnab Interactions of FliJ with the Salmonella type III flagellar export apparatus J. Bacteriol. 185 2003 5546 5554
21. K. Zhu, B. González-Pedrajo, and R.M. Macnab Interactions among membrane and soluble components of the flagellar export apparatus of Salmonella Biochemistry. 41 2002 9516 9524
22. P. Manavalan, and W.C. Johnson Jr Variable selection method improves the prediction of protein secondary structure from circular dichroism data Anal. Biochem. 167 1987 76 85
23. 1, vacuolar, and archaebacterial ATPase subunits J. Bacteriol. 173 1991 3564 3572
24. L. Claret, C.R. Susannah, M. Higgins, and C. Hughes Oligomerization and activation of the FliI ATPase central to bacterial flagellum assembly Mol. Microbiol. 48 2003 1349 1355
25. Y. Saijo-Hamano, K. Namba, and K. Oosawa A new purification method for overproduced proteins sensitive to endogenous proteases J. Struct. Biol. 132 2000 142 146
26. F. Vonderviszt, P. Závodszky, M. Ishimura, H. Uedaira, and K. Namba Structural organization and assembly of flagellar hook protein from Salmonella typhimurium J. Mol. Biol. 284 1995 1399 1416
27. F. Vonderviszt, K. Imada, Y. Furukawa, H. Uedaira, H. Taniguchi, and K. Namba Mechanism of self-association and filament capping by flagellar HAP2 J. Mol. Biol. 284 1998 1399 1416
28. J. Ryu, and R.J. Hartin Quick transformation in Salmonella typhimurium LT2 BioTechniques 8 1990 43 44
29. S. Yamaguchi, H. Fujita, T. Taira, K. Kutsukake, M. Homma, and T. Iino Genetic analysis of three additional fla genes in Salmonella typhimurium J. Gen. Microbiol. 130 1984 3339 3342
30. K. Kutsukake, and T. Iino Refined genetic analysis of the region II che mutants in Salmonella typhimurium Mol. Gen. Genet. 199 1985 406 409