Chip-based gel electrophoresis method for the quantification of half-antibody species in IgG4 and their by- and degradation products

Analytical Biochemistry

Volumn 334, Issue 1, 2004, Pages 81-88

  Forrer, Kurt ^a   Hammer, Sandrine ^a   Helk, Bernhard ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Area percentage quantification; Chip based gel electrophoresis in reduced and nonreduced format; External calibration with IgG4 as a reference standard; Half antibody; IgG4 subclass; Reduction velocities; SDS PAGE; Validation of a chip method

Indexed keywords

ANTIBODIES; CALIBRATION; COVALENT BONDS; DEGRADATION; ELECTROPHORESIS; REDUCTION; SULFUR COMPOUNDS;

AREA PERCENTAGE QUANTIFICATION; GEL ELECTROPHORESIS; HALF-ANTIBODIES; IGG4 SUBCLASS; REFERENCE STANDARD; SDS PAGE; VALIDATION OF A CHIP METHOD;

SODIUM DODECYL SULFATE;

ANTIBODY; DISULFIDE; IMMUNOGLOBULIN G4; IMMUNOGLOBULIN LIGHT CHAIN; MYOSIN;

ARTICLE; CALIBRATION; CHEMICAL BOND; CONCENTRATION (PARAMETERS); DEGRADATION; ERROR; GEL ELECTROPHORESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REDUCTION; REPRODUCIBILITY; SEPARATION TECHNIQUE;

EID: 4644317936     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.07.002     Document Type: Article

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