Clinical analysis of vitamin B6: Determination of pyridoxal 5′-phosphate and 4-pyridoxic acid in human serum by reversed-phase high-performance liquid chromatography with chlorite postcolumn derivatization

Analytical Biochemistry

Volumn 333, Issue 2, 2004, Pages 336-344

  Rybak, Michael E ^a   Pfeiffer, Christine M ^a  

^a Inorg Toxicology Nutrition Branch   (United States)

Author keywords

4 PA; HPLC; Plasma; PLP; Specimen type

Indexed keywords

COENZYMES; ETHYLENEDIAMINETETRAACETIC ACID; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PLASMAS;

4-PA; FLUOROMETRIC DETECTION; HPLC; POST-COLUMN DERIVATIZATION; REPRODUCIBILITIES; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC); SPECIMEN TYPE;

QUALITY CONTROL;

PYRIDOXAL 5 PHOSPHATE; PYRIDOXIC ACID; PYRIDOXINE; SODIUM CHLORITE;

ACCURACY; ARTICLE; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SERUM;

EID: 4644306124     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.06.036     Document Type: Article

References (48)

1. 2 and the pellagra-like dermatitis of rats Nature 244 1934 448 449
2. 6 and related compounds, Eur. J. Biochem. 40 (1973) 325-327
3. 6 R.B. Rucker J.W. Suttie D.B. McCormick L.J. Machlin Handbook of Vitamins third ed. 2001 Marcel Dekker New York 339 396
4. L. Chasan-Taber, J. Selhub, I.H. Rosenberg, M.R. Malinow, P. Terry, P.V. Tishler, W. Willett, C.H. Hennekens, and M.J. Stampfer A prospective study of folate and vitamin B6 and risk of myocardial infarction in U.S. physicians J. Am. Coll. Nutr. 15 1996 136 143
5. M.C. McKinley, H. McNulty, J. McPartlin, J.J. Strain, K. Pentieva, M. Ward, D.G. Weir, and J.M. Scott Low-dose vitamin B-6 effectively lowers fasting plasma homocysteine in healthy elderly persons who are folate and riboflavin replete Am. J. Clin. Nutr. 73 2001 759 764
6. L. Brattström, B. Israelsson, B. Norrving, D. Bergqvist, J. Thorne, B. Hultberg, and A. Hamfelt Impaired homocysteine metabolism in early-onset ancerebral and peripheral occlusive arterial disease: effects of pyridoxine and folic acid treatment Atherosclerosis 81 1990 51 60
7. J.B. Ubbink, W.J.H. Vermaak, A. van der Merwe, P.J. Becker, R. Delport, and H.C. Potgieter Vitamin requirements for the treatment of hyperhomocysteinemia in humans J. Nutr. 124 1994 1927 1933
8. J. Dierkes, M. Kroesen, and K. Pietrzik Folic acid and vitamin B-6 supplementation and plasma homocysteine concentrations in healthy young women Intl. J. Vitam. Nutr. Res. 68 1998 98 103
9. P.J. Kelly, V.E. Shih, J.P. Kistler, M. Barron, H. Lee, R. Mandell, and K.L. Furie Low vitamin B6 but not homocyst(e)ine is associated with increased risk of stroke and transient ischemic attack in the era of folic acid grain fortification Stroke 34 2003 E51 E54
10. 6 nutrition in the rat J. Nutr. 108 1978 545 553
11. A.L. Black, B.M. Guirard, and E.E. Snell The behavior of muscle phosphorylase as a reservoir for vitamin B6 in the rat J. Nutr. 108 1978 670 677
12. S.P. Coburn, P.J. Ziegler, D.L. Costill, J.D. Mahuren, W.J. Fink, W.E. Schaltenbrand, T.A. Pauly, D.R. Pearson, P.S. Conn, and T.R. Guilarte Response of vitamin B-6 content of muscle to changes in vitamin B-6 intake in men Am. J. Clin. Nutr. 53 1991 1436 1442
13. J.E. Leklem Vitamin B-6: a status report J. Nutr. 120 1990 S1503 S1507
14. 6 supplements in humans J. Am. Coll. Nutr. 14 1995 579 586
15. A. Petidier, S. Rubio, A. Gómez-Henz, and M. Valcárcel Simultaneous and direct determination of pyridoxal, pyridoxal-5′- phosphate, and pyridoxic acid in serum by derivative synchronous fluorescence spectroscopy Anal. Biochem. 157 1986 212 220
16. 6 vitamers Anal. Chim. Acta 261 1992 269 274
17. J.J. Berzas Nevado, J.A. Murillo Pulgará-n, and M.A. Gómez Laguna Simultaneous determination of pyridoxal and pyridoxine by different spectrofluorimetric techniques Talanta 42 1995 129 136
18. A.P.H. Phan, T.T. Ngo, and H.M. Lenhoff Tyrosine decarboxylase spectrophotometric assay and application in determining pyridoxal-5′- phosphate Appl. Biochem. Biotech. 8 1983 127 133
19. Q. Han, M. Xu, L. Tang, X. Tan, X. Tan, Y. Tan, and R.M. Hoffman Homogeneous, nonradioactive, enzymatic assay for plasma pyridoxal 5-phosphate Clin. Chem. 48 2002 1560 1564
20. T.R. Guilarte Radiometric microbiological assay of vitamin B6â€"assay simplification and sensitivity study J. Assoc. Off. Anal. Chem. 66 1983 58 61
21. J.F. Gregory III Determination of pyridoxal 5′-phosphate as the semicarbazone derivative using high-performance liquid chromatography Anal. Biochem. 102 1980 374 379
22. J.T. Vanderslice, and C.E. Maire Liquid chromatographic separation and quantification of B6 vitamers at plasma concentration levels J. Chromatogr. 196 1980 176 179
23. 6) in whole blood by high-performance liquid chromatography (HPLC) Intl. J. Vitam. Nutr. Res. 51 1981 216 222
24. 6 vitamer analysis in human plasma by high performance liquid chromatography: a preliminary report Am. J. Clin. Nutr. 34 1981 947 950
25. S.P. Coburn, and J.D. Mahuren A versatile cation-exchange procedure of measuring the seven major forms of vitamin B6 in biological samples Anal. Biochem. 129 1983 310 317
26. 6 nutritional status J. Chromatogr. 342 1985 277 284
27. 6 vitamers in plasma by reversed-phase column liquid chromatography J. Chromatogr. 380 1986 67 75
28. 6 compounds in plasma Clin. Chem. 35 1989 241 245
29. D. Hess, and J.P. Vuilleumier Assay of pyridoxal 5’- phosphate, pyridoxal and pyridoxic acid in biological material Intl. J. Vitam. Nutr. Res. 59 1989 338 343
30. H. Millart, and D. Lamiable Determination of pyridoxal 5′-phosphate in human serum by reversed-phase high-performance liquid chromatography combined with spectrofluorimetric detection of 4-pyridoxic acid 5′-phosphate as a derivative Analyst 114 1989 1225 1228
31. D.A. Sampson, and D.K. O' Connor Analysis of B-6 vitamers and pyridoxic acid in plasma, tissues, and urine using high performance liquid chromatography Nutr. Res. 9 1989 259 272
32. 6 in human serum J. Liquid Chromatogr. 15 1992 897 914
33. 6 vitamers and pyridoxic acid in biological samples J. Chromatogr. 578 1992 45 51
34. M. Kimura, K. Kanehira, and K. Yokoi Highly sensitive and simple liquid chromatographic determination in plasma of B6 vitamers, especially pyridoxal 5′-phosphate J. Chromatogr. A 722 1996 295 301
35. 6 deficiency? Eur. J. Clin. Nutr. 53 1999 448 455
36. C.J. Bates, K.D. Pentieva, N. Matthews, and A. Macdonald A simple, sensitive, and reproducible assay for pyridoxal 5′-phosphate and 4-pyridoxic acid in human plasma Clin. Chim. Acta 280 1999 101 111
37. C.L. Deitrick, R.E. Katholi, D.J. Huddleston, K. Hardiek, and L. Burrus Clinical adaptation of a high-performance liquid chromatographic method for the assay of pyridoxal 5′-phosphate in human plasma J. Chromatogr. B 751 2001 383 387
38. M.R. Bisp, M.V. Bor, E.-M. Heinsvig, M.A. Kall, and E. Nexø, Determination of vitamin B6 vitamers and pyridoxic acid in plasma: Development and evaluation of a high-performance liquid chromatographic assay Anal. Biochem. 305 2002 82 89
39. S.P. Coburn, J.D. Mahuren, W.E. Schaltenbrand, and K.L. Ericson Cation exchange HPLC of pyridoxal 5′-phosphate: improvements in sensitivity of detection and extraction from erythrocytes FASEB J. 15 2001 A963
40. 6 in cooked sausages J. Agric. Food Chem. 49 2001 38 41
41. J.K. Taylor Quality Assurance of Chemical Measurements 1987 Lewis Publishers Boca Raton, FL pp. 79â€"82
42. 6 to laboratory light conditions J. Assoc. Off. Anal. Chem. 62 1979 1170 1173
43. J. Leinert, I. Simon, and D. Hötzel Methods and their evaluation to estimate the vitamin B6 status in human subjects: V. Serum PLP determinationâ€"method and comparison of methods Intl. J. Vit. Nutr. Res. 53 1983 156 165
44. V.M. Camp, J. Chipponi, and B.A. Faraj Radioenzymatic assay for direct measurement of plasma pyridoxal 5′-phosphate Clin. Chem. 29 1983 642 644
45. M.P. Howard, M.A. Andon, and R.D. Reynolds Long-term stability of pyridoxal phosphate in frozen human plasma Fed. Proc. 42 1983 1328 1328
46. M.W. Borschel, A. Kirksey, and B.R. Hamaker A micromethod for determination of plasma pyridoxal phosphate and its use in assessment of storage stability of the vitamer J. Pediatr. Gastr. Nutr. 6 1987 409 413
47. M.C. Ocke, J. Schrijver, G.L. Obermennderboer, B.P.M. Bloemberg, G.R.M.M. Haenen, and D. Kromhout Stability of blood (pro)vitamins during 4 years of storage at -20°C: Consequences for epidemiologic research J. Clin. Epidemiol. 48 1995 1077 1085
48. 6 treatment: a randomized placebo-controlled study Clin. Chem. 49 2003 155 161