Structural analysis of the acetaldehyde dehydrogenase activity of Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2), a member of the ADHE enzyme family

Molecular and Biochemical Parasitology

Volumn 137, Issue 2, 2004, Pages 201-205

  Chen, Minghe ^a   Li, Ellen ^a   Stanley Jr , Samuel L ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Acetaldehyde dehydrogenase; ADHE; Alcohol dehydrogenase; Entamoeba histolytica; Entamoeba histolytica alcohol dehydrogenase 2

Indexed keywords

ALCOHOL DEHYDROGENASE; ALDEHYDE DEHYDROGENASE; PROTOZOAL PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; ENTAMOEBA HISTOLYTICA; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN FUNCTION; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

ALCOHOL DEHYDROGENASE; ALDEHYDE OXIDOREDUCTASES; AMINO ACID SEQUENCE; ANIMALS; ENTAMOEBA HISTOLYTICA; ESCHERICHIA COLI; GENES, PROTOZOAN; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MULTIENZYME COMPLEXES; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

ENTAMOEBA; ENTAMOEBA HISTOLYTICA;

EID: 4644265219     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2004.06.002     Document Type: Article

References (14)

1. Reeves R.E. Metabolism of Entamoeba histolytica Schaudinn, 1903. Adv. Parasitol. 23:1984;105-142
2. Yang W., Li E., Kairong T., Stanley S.L. Jr. Entamoeba histolytica has an alcohol dehydrogenase homologous to the multifunctional adhE gene product of Escherichia coli. Mol. Biochem. Parasitol. 64:1994;253-260
3. Lo H.-S., Reeves R.E. Pyruvate-to-ethanol pathway in Entamoeba histolytica. Biochem. J. 171:1978;225-230
4. Rosenthal B., Mai Z.M., Caplivski D., Ghosh S., De la Vega H., Graf T., et al. Evidence for the bacterial origin of genes encoding fermentation enzymes of the amitochondriate protozoan parasite Entamoeba histolytica. J. Bacteriol. 179:1997;3736-3745
5. Goodlove P.E., Cunningham P.R., Parker J., Clark D.P. Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli. Gene. 85:1989;209-214
6. Espinosa A., Yan L., Zhang Z., Foster L., Clark D., Li E., et al. The bifunctional Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) protein is necessary for amebic growth and survival and requires an intact C-terminal domain for both alcohol dehydrogenase and acetaldehyde dehydrogenase activity. J. Biol. Chem. 276:2001;20136-20143
7. Yong T.-S., Li E., Clark D., Stanley S.L. Jr. Complementation of a Escherichia coli adhE mutant by the Entamoeba histolytica EhADH2 gene provides a method for the identification of new anti-amebic drugs. Proc. Natl. Acad. Sci. USA. 93:1996;6464-6469
8. +- dependent acetaldehyde/alcohol dehydrogenase from Entamoeba histolytica. Biochem. J. 303:1994;743-748
9. Liu Z.J., Sun Y.J., Rose J., Chung Y.J., Hsiao C.D., Chang W.R., et al. The first structure of an aldehyde dehydrogenase reveals novel interactions between NAD and the Rossmann fold. Nat. Struct. Biol. 4:1997;317-326
10. Perozich J., Nicholas H., Wang B., Lindahl R., Hempel J. Relationships within the aldehyde dehydrogenase extended family. Protein Sci. 8:1999;137-146
11. Boxma B., Voncken F., Jannink S., Van Alen T., Akhmanova A., Van Weelden S.W., et al. The anaerobic chytridiomycete fungus Piromyces sp. E2 produces ethanol via pyruvate:formate lyase and an alcohol dehydrogenase E. Mol. Microbiol. 51:2004;1389-1399
12. Rose J.P., Hempel J., Kuo I., Lindahl R., Wang B.C. Preliminary crystallographic analysis of class 3 rat liver aldehyde dehydrogenase. Proteins. 8:1990;305-308
13. Sanchez L.B. Aldehyde dehydrogenase (CoA-acetylating) and the mechanism of ethanol formation in the amitochondriate protist, Giardia lamblia. Arch. Biochem. Biophys. 354:1998;57-64
14. Atteia A., van Lis R., Mendoza-Hernandez G., Henze K., Martin W., Riveros-Rosas H., et al. Bifunctional aldehyde/alcohol dehydrogenase (ADHE) in chlorophyte algal mitochondria. Plant Mol. Biol. 53:2003;175-188