Field application of autometallography and X-ray microanalysis using the digestive gland of the common mussel

Ecotoxicology and Environmental Safety

Volumn 59, Issue 1, 2004, Pages 31-37

  Dimitriadis, V K ^a   Papadaki, M ^a  

^a ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

Author keywords

Autometallography; Digestive gland; Heavy metals; Mussel; Mytilus galloprovincialis; X ray microanalysis

Indexed keywords

EPOXY RESIN; HEAVY METAL;

DIGESTION; ECOTOXICOLOGY; FIELD METHOD; HEAVY METAL; X-RAY;

ANIMAL TISSUE; ARTICLE; AUTOMETALLOGRAPHY; BIOLOGICAL MONITORING; CONTROLLED STUDY; ELECTRON MICROSCOPY; EXOCRINE GLAND; HISTOLOGY; LYSOSOME; MUSSEL; NONHUMAN; POPULATION DISTRIBUTION; ULTRASTRUCTURE; X RAY MICROANALYSIS;

ANIMALS; BASOPHILS; BIVALVIA; DIGESTIVE SYSTEM; ENVIRONMENTAL MONITORING; EPOXY RESINS; FROZEN SECTIONS; GREECE; LYSOSOMES; METALS, HEAVY; MICROSCOPY, ELECTRON; PARAFFIN EMBEDDING; SEAWATER; SPECTROMETRY, X-RAY EMISSION; WATER POLLUTANTS, CHEMICAL;

EURASIA; EUROPE; GREECE; SOUTHERN EUROPE;

MYTILUS; MYTILUS GALLOPROVINCIALIS;

EID: 4644229904     PISSN: 01476513     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ecoenv.2003.11.005     Document Type: Article

References (36)

1. Anagnostou C., Georgakopoulou-Gregoriadou E., Zenetos A., Kamberi E., Karageorgis A., Kontogiannis C., Pagou K., Panagiotidis P., Pancucci A., Siokou I., Symbura N., Chathianestis I., Psyllidou-Giuranovits R. The state of the marine ecosystem of the bay and the Gulf of Thessaloniki. the reaction capacity of the ecosystem Proc. 5th Panellenic Symp. Oceanogr. Fish. 1:1997;83-86
2. Anagnostou C., Kamberi H., Karageorgis A. Horizontal and vertical distribution in heavy metals in sediments from Thermaikos Gulf. Rapp. Comm. Int. Mer. Medit. 35:1998;222
3. Andersen J.T., Baatrup E. Ultrastructural localization of mercury accumulations in the gills, hepatopancreas, midgut and antennal glands of the brown shrimp, Crangon crangon. Aquat. Toxicol. 13:1988;309-324
4. Cajaraville M.P., Robledo Y., Etxeberria M., Marigomez I. Cellular biomarkers as useful tools in the biological monitoring of environmental pollution. molluscan digestive lysosomes Cajaraville M.P. Cell Biology in Environmental Toxicology. 1995;29-55 Univ. of the Basque Country Press Service, Bilbo
5. Chandler J.A. X-Ray microanalysis in the electron microscope. Glauert A.M. Practical Methods in Electron Microscopy. 1977;320-541 North-Holland, Amsterdam
6. Danscher G. Autometallography. A new technique for light and electron microscopic visualisation of metals in biological tissue (gold, silver, metal sulphides and metal selenides). Histochemistry. 81:1984;331-335
7. Danscher G., Möller-Madsen B. Silver amplification of mercury sulfide and selenide. a histochemical method for light and electron localization of mercury in tissue J. Histochem. Cytochem. 33:1985;219-228
8. Danscher G., Montagnese C. Autometallographic localization of synaptic vesicular zinc and lysosomal gold, silver and mercury. J. Histotechnol. 17:1994;15-22
9. Danscher G., Norgaard J.O.R. Ultrastructural autometallography. a method for silver amplification of catalytic metals J. Histochem. Cytochem. 33:1985;706-710
10. Domouhtsidou G., Dimitriadis V.K. Ultrastructural localization of heavy metals (Hg, Ag, Pb and Cu) in gills and digestive gland of mussels, Mytilus galloprovincialis (L.). Arch. Environ. Contam. Toxicol. 38:2000;472-478
11. Domouhtsidou G., Dimitriadis V.K. Lysosomal and lipid responses in the digestive gland of the common mussel Mytilus galloprovincialis as biomarkers of environmental stress. Environ. Pollut. 115:2001;123-137
12. George S.G., Viarengo A. A model for heavy metal homeostasis and detoxication in mussels. Vernberg F.J., Thurberg F.P., Calabrese A., Vernberg W.B. Marine Pollution and Physiology: Recent Advances. 1985;125-143 Univ. of South Carolina Press, Columbia
13. Glauert, A.M. (Ed.), 1977. Practical Methods in Electron microscopy, Vol. 6. North-Holland, Amsterdam, pp. 110-117.
14. Hemelraad J., Herwig H.J. Cadmium kinetics in freshwater clams. IV. Histochemical localization of cadmium in Anodonta cygnea and Anodonta anatina exposed to cadmium chloride. Arch. Environ. Contam. Toxicol. 17:1988;337-343
15. Herwig H.J., Brands F., Kruitwagen E., Zandee D.I. Bioaccumulation and histochemical localization of cadmium in Dreissena polymorpha exposed to cadmium chloride. Aquat. Toxicol. 15:1989;269-286
16. Kamarianos, A., Karamanlis, X., Galoupi, E., 2002. Pollution of coastal areas of N. Greece by organochlorine pesticides and Polychlorinated Biphenyls (PCBs), (1991-1996). In: Proceedings, 1st Conference of Macedonia, 1-4 March 2002, Thessaloniki, p. 116.
17. Marigomez I.A., Angulo E., Moya J. Copper treatment of the digestive gland of the slug Arion ater 1. Bioassay conduction and histochemical analysis. Bull. Environ. Contam. Toxicol. 36:1986;600-607
18. Marigomez I., Soto M., Cajaraville M.P. Morphofunctional patterns of cell and tissue systems involved in metal handling and metabolism. Cajaraville M.P. Cell Biology in Environmental Toxicology. 1995;89-134 Univ. of the Basque Country Press Service, Bilbo
19. Marigomez I., Soto M., Kortabitarte M. Tissue-level biomarkers and biological effect of mercury on sentinel slugs, Arion ater. Arch. Environ. Contam. Toxicol. 31:1996;54-62
20. Mason A.Z., Simkiss K., Ryan K.P. The ultrastructural localization of metals in speciments of Littorina littorea collected from clean and polluted sites. J. Mar. Biol. Assoc. UK. 64:1984;699-720
21. Morgan A.Z. X-ray Microanalysis in the Electron Microscopy for Biologists. 1985;Oxford Univ. Press, New York
22. Morton B.S. Feeding and digestion in Bivalves. Saleuddin A.S.M., Wilburg M. The Mollusca, Physiology. Vol. 5:1983;563-586 Academic Press, New York
23. Norgaard J.O.R., Möller-Madsen B., Danscher G. Autometallographical localization of mercury in rat kidney. interaction between mercury and selenium Grawmann M., Drukker J. Histo- and Cytochemistry as a Tool in Environmental Toxicology. Vol. 23:1991;187-193 Fisher Verlag, Stuttgart, New York
24. Nott J.A., Langston W.J. Cadmium and the phosphate granules in Littorina littorea. J. Mar. Biol. Assoc. UK. 69:1989;219-227
25. Nott J.A, Bebianno M.J., Langston W.J., Ryan K.P. Cadmium in the gastropod Littorina littorea. J. Mar. Biol. Assoc. UK. 73:1993;655-665
26. Owen G. Lysosomes, peroxisomes and bivalves. Sci. Prog. Oxf. 60:1972;299-318
27. Pearse, A.G., 1980. Histochemistry: Theoretical and Applied, Vol. 1. Preparative and Optical Technology, 4th Edition. Churchill Livingstone, New York, Edinburgh, London.
28. Reico A., Marigomez J.A., Angulo E., Moya J. Zinc treatment of the digestive gland of the slug Arion ater (L.). 1. Cellular distribution of Zinc and Calcium Bull. Environ. Contam. Toxicol. 41:1988;858-864
29. Soto M., Marigomez I. Metal bioavailability assessment in "mussel-watch" programmes by automated image analysis of autometallographical black silver deposits (BSD) in digestive cell lysosomes. Mar. Ecol. Prog. Ser. 56:1997;141-150
30. Soto M., Cajaraville M.P., Angulo E., Marigomez I. Autometallographic localization of protein-bound copper and zinc in the common winkle, Littorina littorea. a light microscopical study Histochem. J. 28:1996;689-701
31. Soto M., Cajaraville M.P., Marigomez I. Tissue and cell distribution of copper, zinc and cadmium in the mussel, Mytilus galloprovincialis, determined by autometallography. Tissue Cell. 28:1996;557-568
32. Viarengo A. Heavy metals in marine invertebrates. mechanisms of regulation and toxicity at the cellular level CRC Crit. Rev. Aquat. Sci. 1:1989;295-317
33. Viarengo A., Nott J.A. Mechanisms of heavy metal cation homeostasis in marine invertebrates. Comp. Biochem. Physiol. 104:1993;355-372
34. Voutsinou-Taliadouri, F., Hatzianestis, I., Georgakopoulou-Gregoriadou, E., 1998. Trace elements, pesticides and PCBs levels in sediments of a bay influenced by anthropogenic activities (Thermaikos Bay, N.W. Aegean Sea). In: International Symposium on Marine Pollution, Extended Synopses, Monaco, pp. 680-688.
35. Zahariadis, G., Stratis, J., 1995. Heavy metals and nutrients. In: MED-POL Program: Monitoring in Thermaikos Gulf, Annual Report. Aristotle University of Thessaloniki, Greek Ministry of the Environment.
36. Zar Z.H. Biostatistical Analysis. 1984;Prentice-Hall International, Engelwood Cliffs, NJ