The importance of and a method for including transfection efficiency into real-time PCR data analyses

Biotechnology and Bioengineering

Volumn 100, Issue 4, 2008, Pages 765-772

  Godbey, W T ^a   Zhang, Xiujuan ^a   Chang, Fengqi ^a  

^a TULANE UNIVERSITY   (United States)

Author keywords

Delta Delta; Gene delivery; Polymerase chain reaction; Real time PCR; Transfection efficiency

Indexed keywords

ANALYSIS METHODS; APPLIED (CO); CELLULAR RESPONSES; ENVIRONMENTAL STIMULUS; EXPRESSION LEVELS; GENE DELIVERY; POLYMERASE CHAIN REACTION (RT-PCR); REAL-TIME PCR; RELATIVE CHANGES; REVERSE TRANSCRIPTASE (RT); TRANSFECTION EFFICIENCY; WIDE-RANGE;

ADDITION REACTIONS; DATA PROCESSING; FUNCTION EVALUATION; GENE EXPRESSION; GENE TRANSFER; MATHEMATICAL MODELS; NUCLEIC ACIDS; RNA;

COSMIC RAYS;

COMPLEMENTARY DNA; PRIMER DNA; RNA; RNA DIRECTED DNA POLYMERASE;

ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; ENVIRONMENTAL STRESS; ENZYME ACTIVITY; GENE EXPRESSION; GENE TARGETING; GENETIC TRANSDUCTION; GENETIC TRANSFECTION; MATHEMATICAL MODEL; MOUSE; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION;

3T3 CELLS; ANIMALS; CALIBRATION; DATA INTERPRETATION, STATISTICAL; FLUORESCENT DYES; GENE DOSAGE; GENE EXPRESSION; GENE EXPRESSION PROFILING; MICE; MODELS, THEORETICAL; POLYMERASE CHAIN REACTION; REFERENCE VALUES; RESEARCH DESIGN; RNA; TAQ POLYMERASE; TRANSFECTION; VALIDATION STUDIES AS TOPIC;

EID: 46349107250     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21811     Document Type: Article

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