Purification and properties of Rhizobial DehL expressed in Escherichia coli

African Journal of Biotechnology

Volumn 7, Issue 12, 2008, Pages 1944-1949

  Huyop, Fahrul ^a   Rashid, Nooraini Abdul ^a   Wahab, Roswanira A B ^a   Cooper, Ronald A ^b  

^a UNIVERSITI TEKNOLOGI MALAYSIA   (Malaysia)

^b UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

Dehalogenase; Dehl; Rhizobial

Indexed keywords

BACTERIAL ENZYME; DALAPON; DEHL ENZYME; DICHLOROACETIC ACID; DIMER; MONOCHLOROPROPIONATE; MONOCHLOROPROPIONATE DEHALOGENASE; PROPIONIC ACID DERIVATIVE; TRICHLOROACETIC ACID;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE; GEL FILTRATION; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; RHIZOBIUM; SEQUENCE HOMOLOGY;

ESCHERICHIA COLI; RHIZOBIUM SP.;

EID: 46349102896     PISSN: None     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Allison N (1981). Bacterial degradation of halogenated aliphatic acids. PhD Thesis, Trent Polytechnic, Nottingham, UK.
2. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990). Basic local alignment search tools. J. Mol. Biol. 215: 403-410.
3. Bergman JG, Sanik J (1957). Determination of trace amounts of chlorine in naphtha. Anal. Chem. 29: 241-243.
4. Cairns SS (1994). The cloning and analysis of Rhizobium dehalogenase genes. PhD Thesis, University of Leicester, UK.
5. Corpet F (1988). Multiple sequence alignment with hierarchical clustering. Nucl. Acids Res. 16 (22): 10881-10890.
6. Goldman P, Milne GWA, Keister DB (1968). Carbon halogen bond cleavage III. Studies on bacterial halidohydrolases. J. Biol. Chem. 243: 428-434.
7. Gough JA, Murray, NE (1983). Sequence diversity among related genes for recognition of specific targets in DNA molecules. J. Mol. Biol. 166: 1-19.
8. Hareland WA, Crawford RL, Chapman PJ, Dagley S (1975). Metabolic function and properties of a 4-hydroxyphenyl-acetic acid 1-hydroxylase from Pseudomonas acidovorans. J. Bacteriol. 121:272-285.
9. Higgins TP, Hope SJ, Effendi AJ, Dawson SS, Dancer BN (2002). Biochemical and molecular characterisation of the 2,3-dichloro-1-propanol dehalogenase and stereospecific haloalkanoic dehalogenases from a versatile Agrobacterium sp. Biodegradation, 16: 485-492.
10. Huyop FZ, Yusn TY, Ismail M, Wahab RA, Cooper RA (2004). Overexpression and characterisation of non-stereospecific haloacid Dehalogenase E (DehE) of Rhizobium sp. Asia Pac. J. Mol. Biol. Biotechnol. 12: 15-20.
11. Janssen DB, Oppentocht JE, Poelarends GJ (2001). Microbial dehalogenation. Curr. Opin. Biotechnol. 12(3): 254-258.
12. Janssen DB, Pries F, Van der Ploeg J, Kazemier B, Terpstra P, Witholt B (1989). Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene. J. Bacteriol. 171(12): 6791-6799.
13. Jones DHA, Barth PT, Byrom D, Thomas CM (1992). Nucleotide sequence of the structural gene encoding a 2-haloalkanoic acid dehalogenase of Pseudomonas putida strain AJ1 and purification of the encoded protein. J. Gen. Microbiol. 138: 675-683.
14. Kawasaki H, Toyama T, Maeda T, Hishino H, Tonumura K (1994). Cloning and sequence analysis of a plasmid-encoded 2-haloacid dehalogenase gene from Pseudomonas putida no. 109. Biosci. Biotech. Biochem. 58:160-163.
15. Kawasaki H, Tsuda K, Matsushita I, Tonomura K (1992). Lack of homology between two haloacetate dehalogenase genes encoded on a plasmid from Moraxella species strain B. J. Gen. Microbiol. 138:1317-1323.
16. Klages U, Krauss S, Lingens F (1983). 2-haloacid dehalogenase from 4-chlorobenzoate-degrading Pseudomonas sp. CBS3. Hoppe-Seyler's Z. Physiol. Chem. 364: 529-535.
17. Kwok SY, Siu AFM, Ngai AM, Che CM, Tsang JSH (2007). Proteomic analysis of Burkholderia cepacia MBA4 in the degradation of monochloroacetate. Proteomics. 7: 1107-1116.
18. Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 22: 680-685.
19. Leigh JA, Skinner AJ, Cooper RA (1988). Partial purification, stereospecificity and stoichiometry of three dehalogenases from a Rhizobium species. FEMS Microbiol. Lett. 49: 353-356.
20. Little M, Williams PA (1971). A bacterial halidohydrolase. Its purification, some properties and its modification by specific amino acid reagents. Eur. J. Biochem. 21: 99-109.
21. Morsberger FM, Muller R, Otto MK, Lingens S, Klube KD (1991). Purification and characterisation of 2-halocarboxylic acid dehalogenase II from Pseudomonas sp. CBS3. Biol. Chem. Hoppe Seyler. 372: 915-922.
22. Olaniran AO, Anthony I, Ajisebutu OS, Golyshin P, Babalola GO (2002). The aerobic dechlorination activities of two bacterial species isolated from a refuse dumpsite in Nigeria. Int. Microbiol. 5:21-24.
23. Olaniran AO, Pillay D, Pillay B (2004). Haloalkane and haloacid dehalogenases from aerobic bacterial isolates indigenous to contaminated sites in Africa demonstrate diverse substrate specificities. Chemosphere. 55: 27-33.
24. Park C, Kurihara T, Yoshimura T, Soda K, Esaki N (2003). A new D,L-2-haloacid dehalogenase acting on 2-haloacid amides: purification, characterization and mechanism. J. Mol. Catal. B. 23(1): 329-336.
25. Slater JH, Bull AT, Hardman DJ (1997). Microbial dehalogenation of halogenated alkanoic acids. Adv. Micro. Physiol. 38: 133-176.
26. Smith JM, Harrison K, Colby J (1990). Purification and characterization of D-2-haloacid dehalogenase from Pseudomonas putida strain AJ1/23 J. Gen. Microbiol. 136: 881-886.
27. Song JS, Lee DH, Lee K, Kim CK (2003). Characteristics of several bacterial isolates capable of degrading chloroaliphatic compounds via hydrolytic dechlorination . J. Microbiol., 41(4):277-283.
28. Tsang JSH, Sallis PJ, Bull AT, Hardman DJ (1988). A monobromoacetate dehalogenase from Pseudomonas cepacia MBA4. Arch. Microbiol. 150: 441-446.