Construction of cell surface-engineered yeasts displaying antigen to detect antibodies by immunofluorescence and yeast-ELISA

Applied Microbiology and Biotechnology

Volumn 79, Issue 6, 2008, Pages 1019-1026

  Tang, Yu Qian ^a   Han, Shuang Yan ^a   Zheng, Hong ^a   Wu, Lin ^b   Ueda, Mitsuyoshi ^c   Wang, Xiao Ning ^a   Lin, Ying ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^b Chinese Academy of Sciences   (China)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

Human profilin I; Human proteasome subunit alpha 6; Immunofluorescence; Yeast display; Yeast ELISA

Indexed keywords

ANTIGENS; CELL MEMBRANES; FLUORESCENCE; FOOD ADDITIVES; LABELING; MONOCLONAL ANTIBODIES; NUCLEIC ACIDS; ORGANIC ACIDS; SURFACE PROPERTIES; SURFACES; YEAST;

(1 1 0) SURFACE; APPLIED (CO); CELL SURFACES; ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) METHODS; FLUORESCEIN ISOTHIOCYANATE (FTIC); HUMAN PROTEINS; IN ORDER; MONOCLONAL ANTIBODIES (MOABS); PROFILIN; PROTEASOME; PROTEIN FREE; SPRINGER (CO); SURFACE DISPLAYS; WESTERN BLOTTING; YEAST CELLS;

ANTIBODIES;

IMMUNOGLOBULIN G ANTIBODY; PROFILIN;

ANTIBODY; ANTIGEN; DNA; ENZYME; IMMUNIZATION; PROTEIN; YEAST;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL SURFACE; DNA IMMUNIZATION; ENZYME LINKED IMMUNOSORBENT ASSAY; FUSION GENE; IMMUNOFLUORESCENCE TEST; NONHUMAN; WESTERN BLOTTING; YEAST;

ANIMALS; ANTIBODIES; ANTIGENS; BLOTTING, WESTERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GENE EXPRESSION; HUMANS; HYBRIDOMAS; MICE; PROFILINS; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE;

CAPRA HIRCUS;

EID: 46149105175     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1509-z     Document Type: Article

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