A simple, quick, and high-yield preparation of the human thromboxane A 2 receptor in full size for structural studies

Biochemistry

Volumn 47, Issue 26, 2008, Pages 6819-6826

  Ruan, Ke He ^a   Cervantes, Vanessa ^a   Wu, Jiaxin ^a  

^a UNIVERSITY OF HOUSTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; THROMBOXANE A2 RECEPTOR;

ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; BIOPHYSICS; CELL CULTURE; DRUG DESIGN; DRUG SYNTHESIS; EXTRACTION; LIGAND BINDING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTON NUCLEAR MAGNETIC RESONANCE; WESTERN BLOTTING;

ANIMALS; BACULOVIRIDAE; CELL LINE; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE EXPRESSION; GENETIC VECTORS; HUMANS; LIGANDS; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECEPTORS, THROMBOXANE A2, PROSTAGLANDIN H2; SPODOPTERA; TIME FACTORS;

EID: 46049092562     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi702501g     Document Type: Article

References (44)

1. Majerus, P. W. (1983) Arachdonate metabolism in vascular disorders. J. Clin. Invest. 72, 1521-1525.
2. Samuelson, B., Goldyne, M., Granstrom, E., Hamberg, M., Hammarstrom, S., and Malmsten, C. (1978) Prostaglandins and thromboxanes. Annu. Rev. Biochem. 47, 994-1030.
3. Francois, H., Athirakul, K., Mao, L., Rockman, H., and Coffman, T. M. (2004) Role for thromboxane receptors in angiotensin-II-induced hypertension. Hypertension 43, 364-369.
4. Onguru, O., Casey, M. B., Kajita, S., Nakamura, N., and Lloyd, R. V. (2005) Cyclooxygenase-2 and thromboxane synthase in non-endocrine and endocrine tumors: A review. Endocr. Pathol. 16, 253-277.
5. 2 receptor. Nature 349, 617-620.
6. Raychowdhury, M. K., Yukawa, M., Collins, L. J., McGrail, S. H., Kent, K. C., and Ware, J. A. (1994) Alternative splicing produces a divergent cytoplasmic tail in the human endothelial thromboxane A2 receptor. J. Biol. Chem. 269, 19256-19261.
7. Hirata, T., Ushikubi, F., Kakizuka, A., Okuma, M., and Narumiya, S. (1996) Two thromboxane A2 receptor isoforms in human platelets. Opposite coupling to adenylyl cyclase with different sensitivity to Arg60 to Leu mutation. J. Clin. Invest. 97, 949-956.
8. Boie, Y., Sawyer, N., Slipetz, D. M., Metters, K. M., and Abramovitz, M. (1995) Molecular cloning and characterization of the human prostanoid DP receptor. J. Biol. Chem. 270, 18910-18916.
9. Funk, C. D., Furci, L., FitzGerald, G. A., Grygorczyk, R., Rochette, C., Bayne, M. A., Abramovitz, M., Adam, M., and Metters, K. M. (1993) Cloning and expression of CDNA for the human prostaglandin E receptor EP1 subtype. J. Biol. Chem. 268, 26767-26772.
10. 2. Biochem. Biophys. Res. Commun. 197, 263-270.
11. 3 prostanoid receptor. FEBS Lett. 338, 170-174.
12. 3 receptors: Evidence of three variants with differing carboxyl termini. Br. J. Pharmacol. 112, 377-385.
13. 3 subtype from human erythroleukaemia cells. Biochem. J. 298, 263-267.
14. 2. Biochem. Biophys. Res. Commun. 198, 999-1006.
15. Abramovitz, M., Boie, Y., Nguyen, T., Rushmore, T. H., Bayne, M. A., Metters, K. M., Slipetz, D. M., and Grygorczyk, R. (1994) Cloning and expression of a cDNA for tile human prostanoid FP receptor. J. Biol. Chem. 269, 1356-1360.
16. Katsuyama, M., Sugimoto, Y., Namba, T., Irie, A., Negishi, M., Narumiya, S., and Ichikawa, A. (1994) Cloning and expression of a cDNA for the human prostacyclin receptor. FEBS Lett. 344, 74-88.
17. 2 Receptor in Ligand Binding. Arch. Biochem. Biophys. 352, 207-213.
18. Chiang, N., Kan, W. M., and Tai, H. H. (1996) Site-directed mutagenesis of cysteinyl and serine residues of human thromboxane A2 receptor in insect cells. Arch. Biochem. Biophys. 334, 9-17.
19. Zhou, H., Yan, F., and Tai, H. H. (2001) Phosphorylation and desensitization of the human thromboxane receptor-α by G protein-coupled receptor kinases. J. Pharmacol. Exp. Ther. 298, 1243-1251.
20. Pawate, S., Schey, K. L., Meier, G. P., Ullian, M. E., Mais, D. E., and Halushka, P. V. (1998) Expression, characterization, and purification of C-terminally hexahistidine-tagged thromboxane A2 receptors. J. Biol. Chem. 273, 22753-22760.
21. 2 receptor: Implication of the G protein-coupling pocket. Arch. Biochem. Biophys. 470, 73-82.
22. Ruan, K. H., Wu, J., So, S. P., Jenkins, L. A., and Ruan, C. H. (2004) NMR structure of the thromboxane A2 receptor ligand recognition pocket. Eur. J. Biochem. 271, 3006-3016.
23. Geng, L., Wu, J., So, S. P., Huang, G., and Ruan, K. H. (2004) Structural and functional characterization of the first intracellular loop of human thromboxane A2 receptor. Arch. Biochem. Biophys. 423, 253-265.
24. Wu, J., So, S. P., and Ruan, K. H. (2003) Solution structure of the third extracellular loop of human thromboxane A2 receptor. Arch. Biochem. Biophys. 414, 287-293.
25. So, S. P., Wu, J., Huang, G., Huang, A., Li, D., and Ruan, K. H. (2003) Identification of residues important for ligand binding of thromboxane A2 receptor in the second extracellular loop using the NMR experiment-guided mutagenesis approach. J. Biol. Chem. 278, 10922-10927.
26. Ruan, K. H., So, S. P., Wu, J., Li, D., Huang, A., and Kung, J. (2001) Solution structure of the second extracellular loop of human thromboxane A2 receptor. Biochemistry 40, 275-280.
27. Callihan, D., West, J., Kumar, S., Schweitzer, B. I., and Logan, T. M. (1996) Simple, distortion-free homonuclear spectra of peptides and nucleic acids in water using excitation sculpting. J. Magn. Reson., Ser. B 112, 82-85.
28. Urbani, A., and Warne, T. (2005) A colorimetric determination for glycosidic and bile salt-based detergents: Applications in membrane protein research. Anal. Biochem. 336, 117-124.
29. Ouellet, M., Falgueyret, J. P., Ear, P. H., Pen, A., Mancini, J. A., Riendeau, D., and Percival, M. D. (2002) Purification and characterization of recombinant microsomal prostaglandin E synthase-1. Protein Expression Purif. 26, 489-495.
30. Wallecha, A., and Mishra, S. (2003) Purification and characterization of two β-glucosidases from a thermo-tolerant yeast Pichia etchellsii. Biochim. Biophys. Acta 1649, 74-84.
31. Mais, D. E., True, T. A., and Martinelli, M. J. (1992) Characterization by photoaffinity labelling of the human platelet thromboxane A2/prostaglandin H2 receptor: Evidence for N-linked glycosylation. Eur. J. Pharmacol. 227, 267-274.
32. 2 receptor. Biochim. Biophys. Acta 1621, 192-203.
33. Zhang, L., DiLizio, C., Kim, D., Smyth, E. M., and Manning, D. R. (2006) The G12 family of G proteins as a reporter of thromboxane A2 receptor activity. Mol. Pharmacol. 69, 1433-1440.
34. Kühlbrandt, W. (1988) Three-dimensional crystallization of membrane proteins. Q. Rev. Biophys. 21, 429-477.
35. Cohen, L. S., Arshava, B., Estephan, R., Englander, J., Kim, H., Hauser, M., Zerbe, O., Ceruso, M., Becker, J. M., and Naider, F. (2008) Expression and biophysical analysis of two double-transmembrane domain-containing fragments from a yeast G protein-coupled receptor. Biopolymers 90, 117-130.
36. Werner, K., Richter, C., Klein-Seetharaman, J., and Schwalbe, H. (2007) Isotope labeling of mammalian GPCRs in HEK293 cells and characterization of the C-terminus of bovine rhodopsin by high resolution liquid NMR spectroscopy. J. Biomol. NMR 40, 49-53.
37. Zheng, H., Zhao, J., Sheng, W., and Xie, X. Q. (2006) A transmembrane helix-bundle from G-protein coupled receptor CB2: Biosynthesis, purification, and NMR characterization. Biopolymers 83, 46-61.
38. 19F nuclear Overhauser effects in structural studies of the cytoplasmic domain of mammalian rhodopsin. Proc. Natl. Acad. Sci. U.S.A. 98, 4888-4892.
39. Klein-Seetharaman, J., Yanamala, N. V., Javeed, F., Reeves, P. J., Getmanova, E. V., Loewen, M. C., Schwalbe, H., and Khorana, H. G. (2004) Differential dynamics in the G protein-coupled receptor rhodopsin revealed by solution NMR. Proc. Natl. Acad. Sci. U.S.A. 101, 3409-3413.
40. Ratnala, V. R., Kiihne, S. R., Buda, F., Leurs, R., de Groot, H. J., and DeGrip, W. J. (2007) Solid-state NMR evidence for a protonation switch in the binding pocket of the H1 receptor upon binding of the agonist histamine. J. Am. Chem. Soc. 129, 867-872.
41. Ratnala, V. R. (2006) New tools for G-protein coupled receptor (GPCR) drug discovery: Combination of baculoviral expression system and solid state NMR. Biotechnol. Lett. 28, 767-778.
42. Park, S. H., Prytulla, S., De Angelis, A. A., Brown, J. M., Kiefer, H., and Opella, S. J. (2006) High-resolution NMR spectroscopy of a GPCR in aligned bicelles. J. Am. Chem. Soc. 128, 7402-7403.
43. Kiihne, S. R., Creemers, A. F., de Grip, W. J., Bovee-Geurts, P. H., Lugtenburg, J., and de Groot, H. J. (2005) Selective interface detection: Mapping binding site contacts in membrane proteins by NMR spectroscopy. J. Am. Chem. Soc. 127, 5734-5735.
44. Luca, S., White, J. F., Sohal, A. K., Filippov, D. V., van Boom, J. H., Grisshammer, R., and Baldus, M. (2003) The conformation of neurotensin bound to its G protein-coupled receptor. Proc. Natl. Acad. Sci. U.S.A. 100, 10706-10711.