Overexpression, refolding, purification of Erbin PDZ domain from Escherichia coli and preparation of its polyclonal antibody

Preparative Biochemistry and Biotechnology

Volumn 38, Issue 3, 2008, Pages 282-293

  Zhang, H ^a   Yu, M ^a   Hu, M ^a   Qian, L ^a   Chen, L ^a   Shi, M ^a   Shen, B ^a   Guo, N ^a  

^a INSTITUTE OF BASIC MEDICAL SCIENCES   (China)

Author keywords

Antibody; Erbin; Expression; Purification; Refolding

Indexed keywords

ERBB2IP PROTEIN, HUMAN; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN; SIGNAL TRANSDUCING ADAPTOR PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PDZ DOMAIN; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; UPREGULATION;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ANTIBODIES, MONOCLONAL; ESCHERICHIA COLI; PDZ DOMAINS; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; UP-REGULATION;

ESCHERICHIA COLI; PROKARYOTA;

EID: 45849116901     PISSN: 10826068     EISSN: 15322297     Source Type: Journal    
DOI: 10.1080/10826060802165105     Document Type: Article

References (12)

1. Borg, J.P.; Marchetto, S.; Le Bivic, A.; Ollendorff, V.; Jaulin-Bastard, F.; Saito, H.; Fournier, E.; Adélaíde, J.; Margolis, Ben.; Birnbaum, D. ERBIN: a basolateral PDZ protein that interacts with the mammalian ERBB2/HER2 receptor. Nature Cell. Biol. 2000, 2002 (2), 407-414.
2. Favre, B.; Fontao, L.; Koster, J.; Shafaatian, R.; Jaunin, F.; Saurat, J. H.; Sonnenberg, A.; Borradori, L. The hemidesmosomal protein bullous pemphigoid antigen 1 and the integrin β4 subunit bind to ERBIN. Molecular cloning of multiple alternative splice variants of ERBIN and analysis of their tissue expression. J. Biol. Chem. 2001, 276 (35), 32427-32436.
3. Huang, Y.Z.; Wang, Q.; Xiong, W.C.; Mei, L. Erbin is a protein concentrated at postsynaptic membranes that interacts with PSD-95. J. Biol. Chem. 2001, 276 (22), 19318-19326.
4. Birrane, G.; Chung, J.; Ladias, J.A.A. Novel mode of ligand recognition by the Erbin PDZ domain. J. Biol. Chem. 2003, 278 (3), 1399-1402.
5. Warner, D.R.; Pisano, M.M.; Roberts, E.A.; Greene, R.M. Identification of three novel Smad binding proteins involved in cell polarity. FEBS Lett. 2003, 2003 (539), 167-173.
6. Huang, Y.Z.; Zang, M.; Xiong, W.C.; Luo, Z.; Mei, L. Erbin suppresses the MAP kinase pathway. J. Biol. Chem. 2003, 278 (2), 1108-1114.
7. Izawa, I.; Nishizawa, M.; Tomono, Y.; Ohtakara, K.; Takahashi, T.; Inagaki, M. ERBIN associates with p0071, an armadillo protein, at cell-cell junctions of epithelial cells. Genes Cells 2002, 2002 (7), 475-485.
8. Jaulin-Bastard, F.; Arsanto, J.P.; Le Bivic, A.; Navarro, C.; Vély, F.; Saito, H.; Marchetto, S.; Hatzfeld, M.; Santoni, M.J.; Birnbaum, D.; Borg, J.P. Interaction between Erbin and a catenin-related protein in epithelial cells. J. Biol. Chem. 2002, 277 (4), 2869-2875.
9. Laura, R.P.; Witt, A.S.; Held, H.A.; Gerstner, R.; Deshayes, K.; Koehler, M. F.T.; Kosik, K.S.; Sidhu, S.S.; Lasky, L.A. The erbin PDZ domain binds with high affinity and specificity to the carboxyl termini of δ-catenin and ARVCF. J. Biol. Chem. 2002, 277 (15), 12906-12914.
10. Rangwala, R.; Banine, F.; Borg, J.P.; Sherman, L.S. Erbin regulates mitogen-activated protein (MAP) kinase activation and MAP Kinase-dependent interactions between Merlin and adherens junction protein complexes in Schwann cells. J. Biol. Chem. 2005, 280 (12), 11790-11797.
11. Ohno, H.; Hirabayashi, S.; Iizuka, T.; Ohnishi, H.; Fujita, T.; Hata, Y. Localization of p0071-interacting proteins, plakophilin-related armadillorepeat protein-interacting protein (PAPIN) and ERBIN, in epithelial cells. Oncogene 2002, 2002 (21), 7042-7049.
12. Hynes, N.E.; Lane, H.A. ERBB receptors and cancer: the complexity of targeted inhibitors. Nat. Rev. Cancer 2005, 2005 (5), 341-354.