A nitrobenzoduran-conjugated phosphatidylcholine (C12-NBD-PC) as a stain for membrane lamellae for both microscopic imaging and spectroflourimetry

Biotechnic and Histochemistry

Volumn 83, Issue 1, 2008, Pages 25-28

  Hope Roberts, M ^a   Wainwright, M ^b   Horobin, R W ^c  

^a Arcana Scientific and Medical Translations   (United Kingdom)

^b LIVERPOOL JOHN MOORES UNIVERSITY   (United Kingdom)

^c UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Fluorescent label; Lipid; Log P; NBD stain; Partition coefficient; Water octanol

Indexed keywords

FLUORESCENT DYE; FLUOROCHROME; HALOPERIDOL; HOE 33342; IMIPRAMINE; NITROBENZOFURAN CONJUGATED PHOSPHATIDYLCHOLINE; CARBON; PHOSPHATIDYLCHOLINE;

ARTICLE; CELL CULTURE; CHEMICAL ANALYSIS; CHEMICAL STRUCTURE; CLINICAL LABORATORY; CONJUGATION; FLUORESCENCE ANALYSIS; FLUOROCHROME STAINING; HUMAN; LAMELLAR BODY; MICROSCOPY; NOMENCLATURE; NONHUMAN; PARTITION COEFFICIENT; PHYSICAL CHEMISTRY; SPECTROFLUOROMETRY; STAINING; STANDARDIZATION; CELL SURFACE; CHEMISTRY; FLUORESCENCE MICROSCOPY; METHODOLOGY; ULTRASTRUCTURE;

CARBON; CELL SURFACE EXTENSIONS; MICROSCOPY, FLUORESCENCE; PHOSPHATIDYLCHOLINES; SPECTROMETRY, FLUORESCENCE; STAINING AND LABELING;

EID: 45749143230     PISSN: 10520295     EISSN: 14737760     Source Type: Journal    
DOI: 10.1080/10520290801886224     Document Type: Article

References (13)

1. Alakoskela, J-MI and Kinnunen, PKJ (2001) Control of a redox reaction on lipid bilayer surfaces by membrane dipole potential. Biophys. J., 80, pp. 294-304.
2. Alder-Baerens, N., Lisman, Q., Luong, L., Pomorski, T. and Holthuis, JCM (2006) Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles. Mol. Biol. Cell, 17, pp. 1632-1642.
3. Avanti Polar Lipids, Inc
4. Chattopadhyay, A. (1990) Chemistry and biology of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled lipids: Fluorescent probes of biological and model membranes. Chem. Phys. Lipids, 53, pp. 1-15.
5. Hansch, C. and Leo, A. (1995) Exploring QSAR: Fundamentals and Applications in Chemistry and Biology, American Chemical Society, Washington, DC
6. Haugland, RP (1996) Handbook of Fluorescent Probes and Research Chemicals, pp. 291-293. Molecular Probes, Eugene, OR
7. (2002) Conn's Biological Stains: A Handbook of Dyes, Stains and Fluorochromes for Use in Biology and Medicine, p. 88. BIOS Scientific Publishers, Oxford, UK
8. Johnson, I. (1998) Fluorescent probes for living cells. Histochem. J., 30, pp. 123-140.
9. Kasahara, T., Tomita, K., Murano, H., Harada, T., Tsubakimoto, K., Ogihara, T., Ohnishi, S. and Kakinuma, C. (2006) Establishment of an in vitro high-throughput screening assay for detecting phospholipidosis-inducing potential. Tox. Sci., 90, pp. 133-141.
10. Lipsky, NG and Pagano, RE (1985) A vital stain for the Golgi apparatus. Science, 228, pp. 745-747.
11. Maier, O., Oberle, V. and Hoekstra, D. (2002) Fluorescent lipid probes: some properties and applications (a review). Chem. Phys. Lipids, 116, pp. 3-18.
12. Villa, AM, Caporizzo, E., Papagni, A., Miozzo, L., del Buttero, P, Grilli, MD, Amboldi, N., Fazio, F., Doglia, SM and Giglioni, B. (2005) Choline and phosphatidylcholine fluorescent derivatives localization in carcinoma cells studied by laser scanning confocal fluorescence microscopy. Eur. J. Cancer, 41, pp. 1453-1459.
13. Worthman, L-AD, Nag, K., Rich, N., Ruano, MLF, Casals, C., Perez-Gil, J. and Keough, KMW (2000) Pulmonary surfactant protein A interacts with gel-like regions in monolayers of pulmonary surfactant lipid extract. Biophys. J., 79, pp. 2657-2666.