Detection of PEGylated proteins in polyacrylamide gels by reverse staining with zinc and imidazole salts

Electrophoresis

Volumn 29, Issue 11, 2008, Pages 2363-2371

  Hardy, Eugenio ^a   Ramón, José ^b   Saez, Vivian ^a   Báez, Reynier ^a   Tejeda, Yosbel ^a   Ruiz, Amalia ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

^b UNIVERSITY OF HAVANA   (Cuba)

Author keywords

Gel electrophoresis; PEGylated interferon; Protein staining; Reverse staining; Zinc staining

Indexed keywords

ANTIBIOTICS; CHROMATOGRAPHIC ANALYSIS; ELECTROPHORESIS; ENZYMES; GELS; GLYCOPROTEINS; ION CHROMATOGRAPHY; ION EXCHANGE; ZINC;

GEL ELECTROPHORESIS; IMIDAZOL; PEGYLATED; PEGYLATED INTERFERON; PEGYLATED PROTEINS; POLYACRYLAMIDE GELS; PROTEIN STAINING; REVERSE STAINING; SDS-PAGE; ZINC STAINING;

INTERFERONS;

ANAZOLENE SODIUM; CHYMOTRYPSIN; IMIDAZOLE; IODINE; LYSOZYME; OVALBUMIN; PEGINTERFERON ALPHA2B; POLYACRYLAMIDE GEL; ZINC;

ARTICLE; CONCENTRATION PROCESS; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GEL ELECTROPHORESIS; INTERMETHOD COMPARISON; ION EXCHANGE CHROMATOGRAPHY; ISOMER; MOLECULAR WEIGHT; PROTEIN ANALYSIS; QUALITATIVE ANALYSIS; STAINING;

EID: 45749115746     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200700781     Document Type: Article

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