Fluorescent labeling of lignin in the wood pulp fiber wall

Journal of Wood Chemistry and Technology

Volumn 24, Issue 2, 2004, Pages 169-181

  Li, Kecheng ^a   Reeve, Douglas W ^b  

^a UNIVERSITY OF NEW BRUNSWICK   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

Acridine orange; CLSM; Fluorescent labeling; Lignin; Wood pulp fibers

Indexed keywords

COLOR; COMPOSITION; FLUORESCENCE; HARDWOODS; IMAGE ANALYSIS; LASER APPLICATIONS; LIGNIN; MICROSCOPIC EXAMINATION; QUANTUM EFFICIENCY; UNBLEACHED PULP;

ACRIDINE ORANGE; CONFOCAL LASER SCANNING MICROSCOPY; FLUORESCENT LABELING; FLUORESCENT STAINING; LIGNIN CONCENTRATION; METACHROMATIC LABELING; ORTHOCHROMATIC LABELING; WOOD PULP FIBER WALL;

KRAFT PULP;

COLOR DEPTH; FLUORESCENCE; IMAGE ANALYSIS; KRAFT PULPS; LASERS; LIGNIN CONTENT; MICROSCOPY; POPULUS; QUANTUM CHEMISTRY; UNBLEACHED PULPS; WOOD FIBERS;

EID: 4544370302     PISSN: 02773813     EISSN: None     Source Type: Journal    
DOI: 10.1081/WCT-200026572     Document Type: Article

References (18)

1. Lundquist, K.; Josefsson, B.; Nyquist, G. Analysis of lignin products by fluorescence spectroscopy. Holzforschung 1978, 32, 27-32.
2. Lundquist, K.; Egyed, I.; Josefsson, B.; Nyquist, G. Lignin products in pulping liquors and their fluorescence properties. Cell. Chem. Technol. 1981, 15, 669-679.
3. Liu, Y.; Gustafson, R.; Callis, J.; McKean, B. Fluorescence Microphotometry in Determining the Lignin Content of Single Pulp Fibres, Proceedings of 9th ISWPC, Montreal, Canada, June 9-12, 1997; T2-1-T2-5.
4. Stockert, J.C.; Canette, M.; Colman, O.D. Histochemical mechanism for the orthochromatic staining and fluorescence reaction of lignified tissues. Cell. Mol. Biol. 1984, 30 (6), 503-508.
5. Fergus, B.J.; Procter, A.R.; Scott, J.A.N.; Goring, D.A.I. The distribution of lignin in sprucewood as determined by ultraviolet microscopy. Wood Sci. Technol. 1969, 3 (2), 117-138.
6. Saka, S.; Thomas, R. Evaluation of quantitative assay of lignin distribution by SEM-EDXA technique. Wood Sci. Technol. 1982, 16 (1), 1-18.
7. Laine, J.; Stenius, P. Surface characterization of unbleached kraft pulps by means of ESCA. Cellulose 1994, 1 (2), 145-160.
8. Li, K.; Reeve, D.W. The origins of wood fibre surface lignin. J. Pulp Paper Sci. 2002, 28 (11), 1-9.
9. Li, K.; Reeve, D.W. Adsorption of Lignin on Wood Fibre Surfaces, Proceedings of TAPPI International Pulp Bleaching Conference (Oral presentation), Portland, Oregon, May 19-23, 2002; 45-52.
10. Conners, T.E.; Banerjee, S. Surface Analysis of Paper; CRC Press: Boca Raton, FL, 1995.
11. Sheppard, C.J.R.; Shotton, D.M. Confocal Laser Scanning Microscopy; BIOS Scientific Publisher: New York, 1997.
12. Mislankar, A.; Darabie, A.; Reeve, D.W. Detection of ortho-quinones in wood using confocal laser scanning microscopy. J. Pulp Paper Sci. 1997, 23 (2), 73-76.
13. Tasman, J.E.; Berzins, V. The permanganate consumption of pulp material, III. The relationship of kappa number to the lignin content of pulp materials. Tappi J. 1957, 40 (9), 699-704.
14. Kim, H.; Hill, M.K.; Fricke, A.L. Preparation of kraft lignin from black liquor. Tappi J. 1987, 60 (12), 112-116.
15. MSDS, Acridine Orange, Molecular Probes. (2000).
16. Rost, F.W.D. Fluorescence Microscopy; Cambridge University Press: Cambridge, 1992; Vol. I.
17. Rost, F.W.D. Quantitative Fluorescence Microscopy; Cambridge University Press: Cambridge, 1991.
18. Jääskeläinen, A.-S.; Nuopponen, M.; Axwlsson, P.; Tenhunen, M.; Löja, M.; Vuorinen, T. Determination of lignin distribution in pulps by FTIR ATR spectroscopy. J. Pulp Paper Sci. 2003,