Thr/Ser-rich domain of Aspergillus glucoamylase is essential for secretion

Bioscience, Biotechnology and Biochemistry

Volumn 68, Issue 4, 2004, Pages 961-963

  Goto, Masatoshi ^a   Shinoda, Noriko ^a   Oka, Takuji ^a   Sameshima, Yuka ^a   Ekino, Keisuke ^a   Furukawa, Kensuke ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Aspergillus; Bip; Folding; Glucoamylase; O glycosylation

Indexed keywords

BIPA PROTEIN, ASPERGILLUS; FUNGAL PROTEIN; GLUCAN 1,4 ALPHA GLUCOSIDASE; HEAT SHOCK PROTEIN 70; MALTOSE; MESSENGER RNA; SERINE; THREONINE;

ARTICLE; ASPERGILLUS; CHEMISTRY; DRUG EFFECT; ENZYMOLOGY; GENE DELETION; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PROTEIN TERTIARY STRUCTURE; SECRETION;

ASPERGILLUS; FUNGAL PROTEINS; GENE EXPRESSION REGULATION; GLUCAN 1,4-ALPHA-GLUCOSIDASE; HSP70 HEAT-SHOCK PROTEINS; MALTOSE; PROTEIN STRUCTURE, TERTIARY; RNA, MESSENGER; SEQUENCE DELETION; SERINE; THREONINE;

ASPERGILLUS; ASPERGILLUS AWAMORI;

EID: 4544319313     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.68.961     Document Type: Article

References (10)

1. Gunnarsson, A. B., Nilsson, B., and Svensson, S., Structural studies on the O-glycosidically linked carbohydrate chains of glucoamylase G1 from Aspergillus niger. Eur. J. Biochem., 145, 463-467 (1984).
2. Hayashida, S., Nakahara, K., Kuroda, K., Miyata, T., and Iwanaga, S., Structure of the raw-starch-affinity site on the Aspergillus awamori var. kawachi glucoamylase I molecule. Agric. Biol. Chem., 53, 135-141 (1989).
3. Goto, M., Kuwano, E., Kanlayakrit, W., and Hayashida, S., The role of the carbohydrate moiety of a glucoamylase from Aspergillus awamori var. kawachi in the digestion of raw starch. Biosci. Biotechnol. Biochem., 59, 16-20 (1995).
4. Goto, M., Tsukamoto, M., Kwon, I., Ekino, K., and Furukawa, K., Functional analysis of O-linked oligosaccharides in threonine/serine-rich region of Aspergillus glucoamylase by expression in mannosyltransferase-disruptants of yeast. Eur. J. Biochem., 260, 596-602 (1999).
5. Libby, C. B., Cornett, C. A., Reilly, P. J., and Ford, C., Effect of amino acid deletions in the O-glycosylated region of Aspergillus awamori glucoamylase. Protein Eng., 7, 1109-1114 (1994).
6. Semimaru, T., Goto, M., Furukawa, K., and Hayashida, S., Functional analysis of the threonine- and serine-rich Gp-I domain of glucoamylase I from Aspergillus awamori var. kawachi. Appl. Environ. Microbiol., 61, 2885-2890 (1995).
7. Sauer, J., Christensen, T., Frandsen, T. P., Mirgorodskaya, E., McGuire, K. A., Driguez, H., Roepstorff, P., Sigurskjold, B. W., and Svensson, B., Stability and function of interdomain linker variants of glucoamylase 1 from Aspergillus niger. Biochemistry, 40, 9336-9346 (2001).
8. Gething, M. J., and Sambrook, J., Protein folding in the cell. Nature, 355, 33-45 (1992).
9. Kaufman, R. J., Scheuner, D., Schroder, M., Shen, X., Lee, K., Liu, C. Y., and Arnold, S. M., The unfolded protein response in nutrient sensing and differentiation. Nat. Rev. Mol. Cell. Biol., 3, 411-421 (2002).
10. Goto, M., Ekino, K., and Furukawa, K., Expression and functional analysis of a hyperglycosylated glucoamylase in a parental host, Aspergillus awamori var. kawachi. Appl. Environ. Microbiol., 63, 2940-2943 (1997).