A novel hydroxylase from Catharanthus roseus participating in the hydroxylation of 2-hydroxybenzoic acid

Journal of Bioscience and Bioengineering

Volumn 98, Issue 2, 2004, Pages 67-70

  Shimoda, Kei ^a   Kubota, Naoji ^a   Sano, Takayuki ^a   Hirakawa, Hidetada ^b   Hirata, Toshifumi ^c  

^a OITA UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c HIROSHIMA UNIVERSITY   (Japan)

Author keywords

2,5 dihydroxybenzoic acid; 2 hydroxybenzoic acid; Catharanthus roseus; Hydroxylase

Indexed keywords

AFFINITY CHROMATOGRAPHY; CATALYSIS; CELL CULTURE; ENZYMES; HYDROXYLATION; ION EXCHANGE;

ANION EXCHANGE CHROMATOGRAPHY; ENZYME ACTIVITY;

BIOMEDICAL ENGINEERING;

2,5 DIHYDROBENZOIC ACID; BENZOIC ACID DERIVATIVE; COPPER ION; DIVALENT CATION; ENZYME INHIBITOR; FLAVONOID; GENTISIC ACID; HYDROXYAPATITE; HYDROXYBENZOIC ACID; MERCURY; OXYGENASE; UNCLASSIFIED DRUG;

ADSORPTION CHROMATOGRAPHY; AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CATALYSIS; CATHARANTHUS ROSEUS; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SPECIFICITY; HYDROXYLATION; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PH; PLANT CELL; PLANT TISSUE; SEQUENCE HOMOLOGY;

CATHARANTHUS ROSEUS;

EID: 4544296302     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.98.67     Document Type: Article

References (16)

1. Harpel, M. R. and Lipscomb, J. D.: Gentisate 1,2-dioxygenase from Pseudomonas. J. Biol. Chem., 265, 6301-6311 (1990).
2. Groseclose, E. E. and Ribbons, D. W.: 3-Hydroxylase from Pseudomonas aeruginosa. Biochem. Biophys. Res. Commun., 55, 897-903 (1973).
3. Wang, L. H., Hamazah, R. Y., Yu, Y., and Tu, S. C.: Pseudomonas cepacia 3-hydroxybenzoate 6-hydroxylase: induction, purification, and characterization. Biochemistry, 26, 1099-1104 (1987).
4. Yu, Y., Wang, L. H., and Tu, S. C.: Pseudomonas cepacia 3-hydroxybenzoate 6-hydroxylase: stereochemistry, isotope effects, and kinetic mechanism. Biochemistry, 26, 1105-1110 (1987).
5. Rajasekharan, S., Rajasekharan, R., and Vaidyanathan, C. S.: Substrate-mediated purification and characterization of a 3-hydroxybenzoic acid-6-hydroxylase from Micrococcus. Arch. Biochem. Biophys., 278, 21-25 (1990).
6. Suarez, M., Ferrer, E., Pertierra, A. G., and Martin, M.: Purification and characterization of the 3-hydroxybenzoate-6-hydroxylase from Klebsiella pneumoniae. FEMS Microbiol. Lett., 126, 283-290 (1995).
7. Shimoda, K., Yamane, S., Hirakawa, H., Ohta, S., and Hirata, T.: Biotransformation of phenolic compounds by the cultured cells of Catharanthus roseus. J. Mol. Catal. B: Enzym., 16, 275-281 (2002).
8. Hamada, H., Kondo, Y., Ishihara, K., Nakajima, N., Hamada, H., Kurihara, R., and Hirata, T.: Stereoselective biotransformation of limonene and limonene oxide by cyanobacterium, Synechococcus sp. PCC 7942. J. Biosci. Bioeng., 96, 581-584 (2003).
9. Hamada, H., Fuchikami, Y., Ikematsu, Y., Hirata, T., Williams, H., and Scott, A. I.: Hydroxylation of piperitone by cell suspension cultures of Catharanthus roseus. Phytochemistry, 37, 1037-1038 (1994).
10. Schenk, R. U. and Hildebrandt, A. C.: Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot., 50, 199-204 (1972).
11. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
12. Bradford, M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).
13. Miles, E. W.: Modification of histidyl residues in proteins by diethylpyrocarbonate. Methods Enzymol., 47, 431-442 (1977).
14. Schoenbohm, C., Martens, S., Eder, C., Forkmann, G., and Weisshaar, B.: Identification of the Arabidopsis thaliana flavonoid 3′-hydroxylase gene and functional expression of the encoded P450 enzyme. Biol. Chem., 381, 749-753 (2000).
15. Toda, K., Yang, D., Yamanaka, N., Watanabe, S., Harada, K., and Takahashi, R.: A single-base deletion in soybean flavonoid 3′-hydroxylase gene is associated with gray pubescence color. Plant Mol. Biol., 50, 187-196 (2002).
16. Hagmann, M. L., Heller, W., and Grisebach, H.: Induction and characterization of a microsomal flavonoid 3′-hydroxylase from Parsley cell cultures. Eur. J. Biochem., 134, 547-554 (1983).