Culture and expansion of the human embryonic stem cell line HS181, evaluated in a double-color system

Stem Cells and Development

Volumn 13, Issue 4, 2004, Pages 337-343

  Imreh, Marta P ^a   Wolbank, Susanne ^a   Unger, Christian ^a   Gertow, Karin ^a   Aints, Alar ^a   Szeles, Anna ^b   Imreh, Stefan ^b   Hovatta, Outi ^a   Fried, Gabriel ^a   Dilber, Sirac ^a   Ährlund Richter, Lars ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; RED FLUORESCENT PROTEIN;

ARTICLE; CELL CULTURE; CELL DENSITY; CELL DIFFERENTIATION; CELL FUSION; CELL GROWTH; CELL INTERACTION; CELL LINE; CELL LINE HS181; CELL SHAPE; CELL VIABILITY; COLONY FORMATION; COLOR; CONTROLLED STUDY; DOUBLE COLOR SYSTEM; EMBRYO; EMBRYO CELL; EMBRYONIC STEM CELL LINE; FIBROBLAST; FORESKIN FIBROBLAST; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN SITU HYBRIDIZATION; LIFESPAN; MITOSIS RATE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INTERACTION; STEM CELL;

BASE SEQUENCE; CELL COMMUNICATION; CELL CULTURE TECHNIQUES; CELL LINE; DNA PRIMERS; EMBRYO; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; INFANT, NEWBORN; MALE; STEM CELLS; TRANSFECTION;

EID: 4544223191     PISSN: 15473287     EISSN: None     Source Type: Journal    
DOI: 10.1089/scd.2004.13.337     Document Type: Article

References (17)

1. Thomson JA, J Itskovitz-Eldor, SS Shapiro, MA Waknitz, JJ Swiergiel, VS Marshall and JM Jones. (1998). Embryonic stem cell lines derived from human blastocysts. Science 282:1145-1147.
2. Rippon HJ and AE Bishop. (2004). Embryonic stem cells. Cell Prolif 37:23-34.
3. Hovatta O, M Mikkola, K Gertow, AM Stromberg, J Inzunza, J Hreinsson, B Rozell, E Blennow, M Andang and L Ahrlund-Richter. (2003). A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum Reprod 18:1404-1409.
4. Gertow K, S Wolbank, B Rozell, R Sugars, M Andang, CL Parish, M. Imreh, L. Wendel, and L Ahrlund-Richter. (2004). Organized development from human embryonic stem cells after injection into immunodeficient mice. Stem Cells and Development 13:421-435.
5. Szeles A, S Bajalica-Lagercrantz, A Lindblom, T Lushnikova, VI Kashuba, S Imreh, M Nordenskjold, G Klein and ER Zabarovsky. (1996). Mapping of a new MAP kinase activated protein kinase gene (3PK) to human chromosome band 3p21.2 and ordering of 3PK and two cosmid markers in the 3p22-p21 tumour-suppressor region by two-colour fluorescence in situ hybridization. Chromosome Res 4:310-313.
6. Lois C, EJ Hong, S Pease, EJ Brown and D Baltimore. (2002). Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 295:868-872.
7. Terada N, T Hamazaki, M Oka, M Hoki, DM Mastalerz, Y Nakano, EM Meyer, L Morel, BE Petersen and EW Scott. (2002). Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion. Nature 416:542-545.
8. Ringertz NE and RE Savage. (1976). Cell Hybrids. Elsevier Science SL Technology Books, Academic Press, New York.
9. Inzunza J, S Sahlen, K Holmberg, AM Stromberg, H Teerijoki, E Blennow, O Hovatta and H Malmgren. (2004). Comparative genomic hybridization and karyotyping of human embryonic stem cells reveals the occurrence of an isodicentric X chromosome after long-term cultivation. Mol Hum Reprod 10:461-466.
10. Chambers I, D Colby, M Robertson, J Nichols, S Lee, S Tweedie and A Smith. (2003). Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113:643-655.
11. Niwa H, J Miyazaki and AG Smith. (2000). Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genet 24:372-376.
12. Xu C, MS Inokuma, J Denham, K Golds, P Kundu, JD Gold and MK Carpenter. (2001). Feeder-free growth of undifferentiated human embryonic stem cells. Nature Biotechnol 19:971-974.
13. Amit M, C Shariki, V Margulets and J Itskovitz-Eldor. (2004). Feeder layer- and serum-free culture of human embryonic stem cells. Biol Reprod 70:837-845.
14. Rosier ES, GJ Fisk, X Ares, J Irving, T Miura, MS Rao and MK Carpenter. (2004). Long-term culture of human embryonic stem cells in feeder-free conditions. Dev Dynam 229:259-274.
15. Ma Y, A Ramezani, R Lewis, RG Hawley and JA Thomson. (2003). High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors. Stem Cells 21:111-117.
16. Gropp M, P Itsykson, O Singer, T Ben-Hur, E Reinhartz, E Galun and BE Reubinoff. (2003). Stable genetic modification of human embryonic stem cells by lentiviral vectors. Mol Ther 7:281-287.
17. Pfeifer A, M Ikawa, Y Dayn and IM Verma. (2002). Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc Natl Acad Sci USA 99:2140-2145.