Development of a novel chloramphenicol resistance expression plasmid used for genetic complementation of a fliG deletion mutant in Treponema denticola

Infection and Immunity

Volumn 72, Issue 9, 2004, Pages 5493-5497

  Slivienski Gebhardt, Linda L ^a   Izard, Jacques ^a,b   Samsonoff, William A ^a   Limberger, Ronald J ^a  

^a WADSWORTH CENTER   (United States)

^b FORSYTH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORAMPHENICOL;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL FLAGELLUM; BACTERIAL GENE; BACTERIAL GENETICS; CELL MOTILITY; DELETION MUTANT; FLA GENE; FLIG GENE; GENE DELETION; GENE EXPRESSION; GENETIC COMPLEMENTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAPHYLOCOCCAL CHLORAMPHENICOL RESISTANCE GENE; TREPONEMA DENTICOLA;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; CHLORAMPHENICOL; DRUG RESISTANCE, BACTERIAL; GENE DELETION; GENETIC COMPLEMENTATION TEST; HUMANS; MOLECULAR SEQUENCE DATA; PLASMIDS; SEQUENCE ANALYSIS, DNA; TREPONEMA;

EID: 4544219901     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.72.9.5493-5497.2004     Document Type: Article

References (32)

1. Charon, N. W., and S. F. Goldstein. 2002. Genetics of motility and chemotaxis of a fascinating group of bacteria: the spirochetes. Annu. Rev. Genet. 36:47-73.
2. Charon, N. W., S. F. Goldstein, S. M. Block, K. Curci, J. D. Ruby, J. A. Kreiling, and R. J. Limberger. 1992. Morphology and dynamics of protruding spirochete periplasmic flagella. J. Bacteriol. 174:832-840.
3. Chi, B., S. Chauhan, and H. Kuramitsu. 1999. Development of a system for expressing heterologous genes in the oral spirochete Treponema denticola and its use in expression of the Treponema pallidum flaA gene. Infect. Immun. 67:3653-3656.
4. Chi, B., R. J. Limberger, and H. K. Kuramitsu. 2002. Complementation of a Treponema denticola flgE mutant with a novel coumermycin A1-resistant T. denticola shuttle vector system. Infect. Immun. 70:2233-2237.
5. Heinzerling, H. F., M. Olivares, and R. A. Burne. 1997. Genetic and transcriptional analysis of flgB flagellar operon constituents in the oral spirochete Treponema denticola and their heterologous expression in enteric bacteria. Infect. Immun. 65:2041-2051.
6. Heinzerling, H. F., J. E. Penders, and R. A. Burne. 1995. Identification of a fiG homologue in Treponema denticola. Gene 161:69-73.
7. Holt, S. C. 1978. Anatomy and chemistry of spirochetes. Microbiol. Rev. 42:114-160.
8. Irikura, V. M., M. Kihara, S. Yamaguchi, H. Sockett, and R. M. Macnab. 1993. Salmonella typhimurium fliG and fliN mutations causing defects in assembly, rotation, and switching of the flagellar motor. J. Bacteriol. 175:802-810.
9. Izard, J., W. A. Samsonoff, M. B. Kinoshita, and R. J. Limberger. 1999. Genetic and structural analyses of the cytoplasmic filaments of wild-type Treponema phagedenis and a flagellar filament-deficient mutant. J. Bacteriol. 181:6739-6746.
10. Kihara, M., M. Homma, K. Kutsukake, and R. M. Macnab. 1989. Flagellar switch of Salmonella typhimurium: gene sequences and deduced protein sequences. J. Bacteriol. 171:3247-3257.
11. Kihara, M., G. U. Miller, and R. M. Macnab. 2000. Deletion analysis of the flagellar switch protein FliG of Salmonella. J. Bacteriol. 182:3022-3028.
12. Kimsey, R. B., and A. Spielman. 1990. Motility of Lyme disease spirochetes in fluids as viscous as the extracellular matrix. J. Infect. Dis. 162:1205-1208.
13. Kitorinos, A., P. Noble, R. Siboo, and E. C. Chan. 1993. Viscosity-dependent locomotion of oral spirochetes. Oral Microbiol. Immunol. 8:242-244.
14. Li, C., A. Motaleb, M. Sal, S. F. Goldstein, and N. W. Charon. 2000. Spirochete periplasmic flagella and motility. J. Mol. Microbiol. Biotechnol. 2:345-354.
15. Li, H., J. Ruby, N. Charon, and H. Kuramitsu. 1996. Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant. J. Bacteriol. 178:3664-3667.
16. Limberger, R. J. 2004. The periplasmic flagellum of spirochetes. J. Mol. Microbiol. Biotechnol. 7:30-40.
17. Limberger, R. J., and N. W. Charon. 1986. Antiserum to the 33,000-dalton periplasmic-flagellum protein of "Treponema phagedenis" reacts with other treponemes and Spirochaeta aurantia. J. Bacteriol. 168:1030-1032.
18. Limberger, R. J., L. L. Slivienski, J. Izard, and W. A. Samsonoff. 1999. Insertional inactivation of Treponema denticola tap1 results in a nonmotile mutant with elongated flagellar hooks. J. Bacteriol. 181:3743-3750.
19. Lloyd, S. A., H. Tang, X. Wang, S. Billings, and D. F. Blair. 1996. Torque generation in the flagellar motor of Escherichia coli: evidence of a direct role for FliG but not for FliM or FliN. J. Bacteriol. 178:223-231.
20. Lloyd, S. A., F. G. Whitby, D. F. Blair, and C. P. Hill. 1999. Structure of the C-terminal domain of FliG, a component of the rotor in the bacterial flagellar motor. Nature 400:472-475.
21. Loesche, W. J. 1988. The role of spirochetes in periodontal disease. Adv. Dent. Res. 2:275-283.
22. Macnab, R. M. 1992. Genetics and biogenesis of bacterial flagella. Annu. Rev. Genet. 26:131-158.
23. Macnab, R. M. 2003. How bacteria assemble flagella. Annu. Rev. Microbiol. 57:77-100.
24. Minamino, T., R. Chu, S. Yamaguchi, and R. M. Macnab. 2000. Role of FliJ in flagellar protein export in Salmonella. J. Bacteriol. 182:4207-4215.
25. Motaleb, M. A., M. Sal, and N. Charon. 2004. The decrease in FlaA observed in a flaB mutant of Borrelia burgdorferi occurs posttranscriptionally. J. Bacteriol. 186:3703-3711.
26. Ruby, J. D., H. Li, H. Kuramitsu, S. J. Norris, S. F. Goldstein, K. F. Buttle, and N. W. Charon. 1997. Relationship of Treponema denticola periplasmic flagella to irregular cell morphology. J. Bacteriol. 179:1628-1635.
27. Sadziene, A., D. D. Thomas, V. G. Bundoc, S. C. Holt, and A. G. Barbour. 1991. A flagella-less mutant of Borrelia burgdorferi: structural, molecular and in vitro functional characterization. J. Clin. Investig. 88:82-92.
28. Sartakova, M. L., E. Y. Dobrikova, M. A. Motaleb, H. P. Godfrey, N. W. Charon, and F. C. Cabello. 2001. Complementation of a nonmotile flaB mutant of Borrelia burgdorferi by chromosomal integration of a plasmid containing a wild-type flaB allele. J. Bacteriol. 183:6558-6564.
29. Simonson, L. G., C. H. Goodman, J. J. Bial, and H. E. Morton. 1988. Quantitative relationship of Treponema denticola to severity of periodontal disease. Infect. Immun. 56:726-728.
30. Thomas, D. D., M. Navab, D. A. Haake, A. M. Fogelman, J. N. Miller, and M. A. Lovett. 1988. Treponema pallidum invades intracellular junctions of endothelial cell monolayers. Proc. Natl. Acad. Sci. USA 8:3608-3612.
31. Wang, G., C. Barton, and F. G. Rodgers. 2002. Bacterial DNA decontamination for reverse transcription polymerase chain reaction (RT-PCR). J. Microbiol. Methods 51:119-121.
32. Yamaguchi, S., S.-I. Aizawa, M. Kihara, M. Isomura, C. J. Jones, and R. M. Macnab. 1986. Genetic evidence for a switching and energy-transducing complex in the flagellar motor of Salmonella typhimurium. J. Bacteriol. 168:1172-1179.