The origins of enhanced activity in factor VIIa analogs and the interplay between key allosteric sites revealed by hydrogen exchange mass spectrometry

Journal of Biological Chemistry

Volumn 283, Issue 19, 2008, Pages 13378-13387

  Rand, Kasper D ^a,b   Andersen, Mette D ^a   Olsen, Ole H ^a   Jørgensen, Thomas J D ^b   Østergaard, Henrik ^a   Jensen, Ole N ^b   Stennicke, Henning R ^a   Persson, Egon ^a  

^a NOVO NORDISK A S   (Denmark)

^b UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD; COAGULATION; HYDROGEN; MASS SPECTROMETERS; MASS SPECTROMETRY; SPECTROMETERS; SPECTROMETRY; SPECTRUM ANALYSIS;

ACTIVE SITES; ALLOSTERIC SITES; BLOOD COAGULATIONS; CATALYTIC EFFICIENCIES; CONFORMATIONAL EFFECTS; DIFFERENT MECHANISMS; HYDROGEN EXCHANGES; INTERMEDIATE CONFORMATIONS; INTRINSIC ACTIVITIES; SINGLE MUTATIONS; TISSUE FACTORS; VASCULAR INJURIES;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

BLOOD CLOTTING FACTOR 7A; ENZYME PRECURSOR; HYDROGEN; THROMBOPLASTIN;

ARTICLE; BLOOD CLOTTING; CATALYSIS; CRYSTAL STRUCTURE; DEGLYCOSYLATION; ION EXCHANGE; MASS SPECTROMETRY; PEPTIDE ANALYSIS; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; AMINO ACID SEQUENCE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; MUTATION; PROTEIN BINDING; PROTEIN QUATERNARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

ALLOSTERIC SITE; AMINO ACID SEQUENCE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; FACTOR VIIA; HUMANS; HYDROGEN; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN BINDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; THROMBOPLASTIN;

EID: 45149122554     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M709716200     Document Type: Article

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