The functional properties of a truncated form of endothelial cell protein C receptor generated by alternative splicing

Haematologica

Volumn 93, Issue 6, 2008, Pages 878-884

  Molina, Eva ^a   Hermida, José ^a   López Sagaseta, Jacinto ^a   Puy, Cristina ^a   Montes, Ramón ^a,b  

^a UNIVERSITY OF NAVARRA   (Spain)

^b UNIVERSITY OF NAVARRA   (Spain)

Author keywords

Alternative splicing; Endothelial protein C receptor; Endothelium; Factor VIIa; Protein C

Indexed keywords

BLOOD CLOTTING FACTOR 7A; COMPLEMENTARY DNA; ENDOTHELIAL CELL PROTEIN C RECEPTOR; PROTEIN C;

ALTERNATIVE RNA SPLICING; ARTICLE; CANCER CELL; ENDOTHELIUM CELL; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; IMMUNOASSAY; LUNG CANCER; PROTEIN BINDING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SURFACE PLASMON RESONANCE; UMBILICAL VEIN; YEAST;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOOD COAGULATION FACTORS; ENDOTHELIUM, VASCULAR; FACTOR VIIA; GENE EXPRESSION REGULATION; HUMANS; MICE; MICE, INBRED BALB C; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; PROTEIN C; RECEPTORS, CELL SURFACE; UMBILICAL VEINS;

EID: 44949236894     PISSN: 03906078     EISSN: None     Source Type: Journal    
DOI: 10.3324/haematol.12376     Document Type: Article

References (19)

1. Fukudome K, Esmon CT. Identification, cloning, and regulation of a novel endothelial cell protein C/activated protein C receptor. J Biol Chem 1994;269:26486-91.
2. Stearns-Kurosawa DJ, Kurosawa S, Mollica JS, Ferrell GL, Esmon CT. The endothelial cell protein C receptor augments protein C activation by the thrombin-thrombomodulin complex. Proc Natl Acad Sci USA 1996; 93:10212-6.
3. López-Sagaseta J, Montes R, Puy C, Díez N, Fukudome K, Hermida J. Binding of factor VIIa to the endothelial cell protein C receptor reduces its coagulant activity. J Thromb Haemost 2007;5:1817-24.
4. Kurosawa S, Stearns-Kurosawa DJ, Hidari N, Esmon CT. Identification of functional endothelial protein C receptor in human plasma. J Clin Invest 1997;100:411-8.
5. Stearns-Kurosawa DJ, Burgin C, Parker D, Comp P, Kurosawa S. Bimodal distribution of soluble endothelial protein C receptor levels in healthy populations. J Thromb Haemost 2003;1:855-6.
6. Saposnik B, Reny JL, Gaussem P, Emmerich J, Aiach M, Gandrille S. A haplotype of the EPCR gene is associated with increased plasma levels of sEPCR and is a candidate risk factor for thrombosis. Blood 2004;103:1311-8.
7. Qu D, Wang Y, Esmon NL, Esmon CT. Regulated endothelial protein C receptor shedding is mediated by tumor necrosis factor-a converting enzyme/ADAM17. J Thromb Haemost 2007;5:395-402.
8. Uitte de Willige S, Van Marion V, Rosendaal FR, Vos HL, de Visser MC, Bertina RM. Haplotypes of the EPCR gene, plasma sEPCR levels and the risk of deep venous thrombosis. J Thromb Haemost 2004;2:1305-10.
9. Medina P, Navarro S, Estelles A, Vaya A, Woodhams B, Mira Y, et al. Contribution of polymorphisms in the endothelial protein C receptor gene to soluble endothelial protein C receptor and circulating activated protein C levels, and thrombotic risk. Thromb Haemost 2004;91:905-11.
10. Simmonds RE, Lane DA. Structural and functional implications of the intron/exon organization of the human endothelial cell protein C/activated protein C receptor (EPCR) gene: comparison with the structure of CD1/major histocompatibility complex alpha1 and alpha2 domains. Blood 1999;94:632-41.
11. Saposnik B, Lesteven E, Lokajczyk A, Esmon CT, Aiach M, Gandrille S. Alternative mRNA splicing is favored by the A3 haplotype of the EPCR gene PROCR and generates a novel soluble form of EPCR in plasma. Blood 2008;111:3442-51.
12. Perez-Ruiz A, Montes R, Velasco F, Lopez-Pedrera C, Paramo JA, Orbe J, et al. Regulation by nitric oxide of endotoxin-induced tissue factor and plasminogen activator inhibitor-1 in endothelial cells. Thromb Haemost 2002;88:1060-5.
13. Hurtado V, Montes R, Gris JC, Bertolaccini ML, Alonso A, Martinez-Gonzalez MA, et al. Autoantibodies against EPCR are found in antiphospholipid syndrome and are a risk factor for fetal death. Blood 2004; 104:1369-74.
14. Regan LM, Stearns-Kurosawa DJ, Kurosawa S, Mollica J, Fukudome K, Esmon CT. The endothelial cell protein C receptor. Inhibition of activated protein C anticoagulant function without modulation of reaction with proteinase inhibitors. J Biol Chem 1996;271:17499-503.
15. Preston RJ, Ajzner E, Razzari C, Karageorgi S, Dua S, Dahlback B, et al. Multifunctional specificity of the protein C/activated protein C Gla domain. J Biol Chem 2006;281:28850-7.
16. Ghosh S, Pendurthi UR, Steinoe A, Esmon CT, Rao LV. Endothelial cell protein C receptor acts as a cellular receptor for factor VIIa on endothelium. J Biol Chem 2007;282:11849-57.
17. Kurosawa S, Stearns-Kurosawa DJ, Carson CW, D'Angelo A, Esmon CT. Plasma levels of endothelial cell protein C receptor are elevated in patients with sepsis and systemic lupus erythematosus: Lack of correlation with thrombomodulin suggests involvement of different pathological processes. Blood 1998;91:725-7.
18. Boomsma MM, Stearns-Kurosawa DJ, Stegeman CA, Raschi E, Meroni PL, Kurosawa S, et al. Plasma levels of soluble endothelial cell protein C receptor in patients with Wegener's granulomatosis. Clin Exp Immunol 2002;128:187-94.
19. Lavigne-Lissalde G, Cochery-Nouvellon E, Mercier E, Mares P, Gris JC. High plasma levels of endothelial protein C receptor are associated with the risk of unexplained fetal death. J Thromb Haemost 2005;3:393-5.