Purification of his-tagged proteins using fractogel-cobalt

Cold Spring Harbor Protocols

Volumn 3, Issue 3, 2008, Pages

  Tom, Roseanne ^a   Bisson, Louis ^a   Durocher, Yves ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

COBALT; FRACTOGEL; HISTIDINE; RECOMBINANT PROTEIN; RESIN;

ARTICLE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; METHODOLOGY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; STORAGE;

EID: 44949208808     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot4980     Document Type: Article

References (5)

1. Arnau, J., Lauritzen, C., Petersen, G.E., and Pedersen, J. 2006. Current strategies for the use of affinity tag and tag removal for the purification of recombinant proteins. Protein Expr. Purif. 48: 1-13.
2. Baldi, L., Muller, N., Picasso, S., Jacquet, R., Girard, P., Thanh, H.P., Derow, E., and Wurm, F.M. 2005. Transient gene expression in suspension HEK-293 cells: Application to large-scale protein production. Biotechnol. Prog. 21: 148-153.
3. Durocher, Y., Perret, S., and Kamen, A. 2002. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Res. 30: E9. doi: 10.1093/nar/30.2.e9.
4. Jordan, M., Köhne, C., and Wurm, F.M. 1998. Calcium-phosphate mediated DNA transfer into HEK-293 cells in suspension: Control of physicochemical parameters allows transfection in stirred media. Transfection and protein expression in mammalian cells. Cytotechnology 26: 39-47.
5. Schlaeger, E.-J. and Christensen, K. 1999. Transient gene expression in mammalian cells grown in serumfree suspension culture. Cytotechnology 30: 71-83.