Correlation between cell morphology and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes

Biotechnology Letters

Volumn 30, Issue 7, 2008, Pages 1189-1195

  Takagi, Mutsumi ^a   Kitabayashi, Takayuki ^a   Koizumi, Satoru ^a   Hirose, Haruka ^a   Kondo, Shin Ichi ^a   Fujiwara, Masashi ^a   Ueno, Kosei ^a   Hiroaki, Misawa ^a   Hosokawa, Yoichiroh ^b   Masuhara, Hiroshi ^b   Wakitani, Shigeyuki ^c  

^a HOKKAIDO UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c OSAKA CITY UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

Aggrecan; Differentiation; Laser; Mesenchymal stem cell; Morphology; Noninvasive

Indexed keywords

CELL MORPHOLOGY; CHONDROCYTES; MESENCHYMAL STEM CELL; POLYGONAL INDEX;

CELL ADHESION; CELL CULTURE; GENE EXPRESSION; MORPHOLOGY; RNA;

BIOTECHNOLOGY;

AGGRECAN; ALCAM PROTEIN, HUMAN; FETOPROTEIN; LEUKOCYTE ANTIGEN; MESSENGER RNA; NERVE CELL ADHESION MOLECULE; THY 1 ANTIGEN;

ARTICLE; BIOSYNTHESIS; CARTILAGE CELL; CELL DIFFERENTIATION; CELL SHAPE; CYTOLOGY; GENE EXPRESSION REGULATION; HUMAN; MESENCHYMAL STEM CELL; METABOLISM; PHYSIOLOGY;

AGGRECANS; ANTIGENS, CD; ANTIGENS, THY-1; CELL ADHESION MOLECULES, NEURONAL; CELL DIFFERENTIATION; CELL SHAPE; CHONDROCYTES; FETAL PROTEINS; GENE EXPRESSION REGULATION; HUMANS; MESENCHYMAL STEM CELLS; RNA, MESSENGER;

EID: 44949192911     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9683-8     Document Type: Article

References (12)

1. Arai F, Ohneda O, Miyamoto T, Zhang XQ, Suda T (2002) Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation. J Exp Med 195(12):1549-1563
2. Bruder SP, Kurth AA, Shea M, Hayes WC, Jaiswal N, Kadiyala S (1998) Bone regeneration by implantation of purified culture-expanded human mesenchymal stem cells. J Orthop Res 16:155-162
3. Diaz-Romero J, Gaillard JP, Grogan SP, Nesic D, Trub T, Mainil-Varlet P (2005) Immunophenotypic analysis of human articular chondrocytes: changes in surface markers associated with cell expansion in monolayer culture. J Cell Physiol 202:731-742
4. Hosokawa Y, Takabayashi J, Miura S, Shukunami C, Hiraki Y, Masuhara H (2004) Nondestructive isolation of single cultured animal cells by femtosecond laser-induced shockwave. Appl Phys A79:795-798
5. Lee CR, Grodzinsky AJ, Spector M (2003) Modulation of the contractile and biosynthetic activity of chondrocytes seeded in collagen-glycosaminoglycan matrices. Tissue Eng 9(1):27-36
6. Matsuda C, Takagi M, Hattori T, Wakitani S, Yoshida T (2005) Differentiation of human bone marrow mesenchymal stem cells to chondrocytes for construction of three-dimensional cartilage tissue. Cytotechnology 47:11-17
7. Oda R, Suardita K, Fujimoto K, Pan H, Yan W, Shimazu A, Shintani H, Kato Y (2003) Anti-membrane-bound transferrin-like protein antibodies induce cell-shape change and chondrocyte differentiation in the presence or absence of concanavalin A. J Cell Sci 116:2029-2038
8. Stute N, Holtz K, Bubenheim M, Lange C, Blake F, Zander AR (2004) Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use. Exp Hematol 32:1212-1225
9. Takagi M, Nakamura T, Matsuda C, Hattori T, Wakitani S, Yoshida T (2003) In vitro proliferation of human bone marrow mesenchymal stem cells employing donor serum and basic fibroblast growth factor. Cytotechnology 43(1-3):89-96
10. Vogel A, Noack J, Huttman G, Paltauf G (2005) Mechanism of femtosecond laser nanosurgery of cell and tissues. Appl Phys B Laser Opt 81:1015-1047
11. Wagatsuma A, Sadamoto H, Kitahashi T, Lukowiak K, Urano A, Ito E (2005) Determination of the exact copy numbers of particular mRNAs in a single cell by quantitative real-time RT-PCR. J Exp Biol 208:2389-2398
12. Wexler SA, Donaldson C, Denning-Kendall P, Rice C, Bradley B, Hows JM (2003) Adult bone marrow is a rich source of human mesenchymal 'stem' cells but umbilical cordand mobilizedadult blood are not. Br J Haematol 121(2):368-374