Transfection of HEK293-EBNA1 cells in suspension with linear PEI for production of recombinant proteins

Cold Spring Harbor Protocols

Volumn 3, Issue 3, 2008, Pages

  Tom, Roseanne ^a   Bisson, Louis ^a   Durocher, Yves ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; PLASMID DNA; POLYETHYLENEIMINE; RECOMBINANT PROTEIN;

ARTICLE; CELL CULTURE; CELL GROWTH; CELL STRAIN HEK293; CELL STRAIN HEK293 EBNA1; CULTURE TECHNIQUE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; METHODOLOGY; MOLECULAR CLONING; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

EID: 44949163900     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot4977     Document Type: Article

References (4)

1. Baldi, L., Muller, N., Picasso, S., Jacquet, R., Girard, P., Thanh, H.P., Derow, E., and Wurm, F.M. 2005. Transient gene expression in suspension HEK-293 cells: Application to large-scale protein production. Biotechnol. Prog. 21: 148-153.
2. Durocher, Y., Perret, S., and Kamen, A. 2002. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Res. 30: E9. doi: 10.1093/nar/30.2.e9.
3. Jordan, M., Köhne, C., and Wurm, F.M. 1998. Calcium-phosphate mediated DNA transfer into HEK-293 cells in suspension: Control of physicochemical parameters allows transfection in stirred media. Transfection and protein expression in mammalian cells. Cytotechnology 26: 39-47.
4. Schlaeger, E.-J. and Christensen, K. 1999. Transient gene expression in mammalian cells grown in serumfree suspension culture. Cytotechnology 30: 71-83.