Streamlined gene assembly PCR

Cold Spring Harbor Protocols

Volumn 3, Issue 3, 2008, Pages

  Barnes, Wayne M ^a   Frawley, Elaine R ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

DNA; OLIGONUCLEOTIDE;

ARTICLE; CODON; COMPUTER PROGRAM; DNA PURIFICATION; DNA SYNTHESIS; GENE SEQUENCE; GENETIC ENGINEERING; METHODOLOGY; POLYMERASE CHAIN REACTION;

EID: 44949152388     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot4862     Document Type: Article

References (3)

1. Barnes, W.M. and Rowlyk, K.R. 2002. Magnesium precipitate hot start method for PCR. Mol. Cell. Probes 16: 167-471.
2. Baskaran, N., Kandpal, R.P., Bhargava, A.K., Glynn, M.W., Bale, A., and Weissman, S.M. 1996. Uniform amplification of a mix of deoxyribonucleic acids with varying GC content. Genome Res. 6: 633-638.
3. Stemmer, W.P.C., Crameri, A., Ha, K.D., Brennan, T.M., and Heyneker, H.L. 1995. Single-step assembly of a gene and entire place from large numbers of oligodeoxyribonucleotides. Gene 164: 49-53.